Human Phospho-Progesterone R/NR3C3 (S294) Antibody

Catalog #: AF5955 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF5955
AF5955-SP

Detection of Human Phospho-Progesterone RB (S294) and Phospho-Progesterone RA (S130) by Western Blot.

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Human Phospho-Progesterone R/NR3C3 (S294) Antibody Summary

Species Reactivity

Human

Specificity

Detects human Phospho-Progesterone R/NR3C3 (S294) in Western blots.

Source

Polyclonal Rabbit IgG

Purification

Antigen Affinity-purified

Immunogen

Phosphopeptide containing human Progesterone RB S294 site
Accession # P06401

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.5 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human Phospho-Progesterone RB (S294) and Phospho-Progesterone RA (S130) by Western Blot. Western blot shows lysates of T47D human breast cancer cell line untreated (-) or treated (+) with 100 nM synthetic progestin promegestone (R5020) for 60 minutes. PVDF Membrane was probed with 0.5 µg/mL of Rabbit Anti-Human Phospho-Progesterone R/NR3C3 (S294) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5955) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Specific bands were detected for Phospho-Progesterone RB (S294) at approximately 118 kDa and Phospho-Progesterone RA (S130) at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Progesterone R/NR3C3

Progesterone Receptor B (PR-B) is a 118 kDa member of the NR3 subfamily within the nuclear hormone receptor family of proteins. It is expressed in female reproductive tissues as well as neurons throughout the CNS. PR-B is particularly important in the mammary gland where it mediates proliferative responses to progesterone. Human PR‑B is 933 amino acids (aa) in length. It contains an N-terminal regulatory region (aa 1‑566), a DNA binding domain (aa 567‑639), and a steroid‑binding region (aa 681‑933). Ligand binding induces a key phosphorylation event at Ser294 catalyzed by ERK1/2, regulating cell growth, cell adhesion, and gene transcription. An alternate start site at Met165 generates 90 kDa PR-A, an isoform particularly important in the ovary and uterus that insures fertility.

Long Name

Progesterone Receptor

Entrez Gene IDs

5241 (Human)

Alternate Names

NR3C3; NR3C3PRNuclear receptor subfamily 3 group C member 3; PGR; Progesterone R; progesterone receptor; ProgesteroneR