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Human Phospho-VEGFR2/KDR/Flk-1 (Y1214) Antibody

Catalog #: AF1766
13 Citations Datasheet / COA / SDS
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AF1766
AF1766-SP
Detection of Human Phospho-VEGFR2/KDR/Flk‑1 (Y1214) by Western Blot.
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Citations (13)
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Human Phospho-VEGFR2/KDR/Flk-1 (Y1214) Antibody Summary

Species Reactivity
Human
Specificity
Detects human VEGFR2/KDR/Flk‑1 in Western blots when phosphorylated at Y1214.
Source
Polyclonal Rabbit IgG
Purification
Antigen Affinity-purified
Immunogen
Phosphopeptide containing human VEGFR2/KDR/Flk-1 Y1214 site
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
A431 human epithelial carcinoma cell line untreated (-) or treated (+) with 100 μM pervanadate (PV) for 10 minutes
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human Phospho-VEGFR2/KDR/Flk-1 (Y1214) antibody by Western Blot. View Larger

Detection of Human Phospho-VEGFR2/KDR/Flk‑1 (Y1214) by Western Blot. Western Blot shows lysates of A431 human epithelial carcinoma cell line untreated (-) or treated (+) with 100 μM pervanadate (PV) for 10 minutes. PVDF membrane was probed with 1 µg/ml of Rabbit Anti-Human Phospho-VEGFR2/KDR/Flk-1 (Y1214) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1766) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for VEGFR2/KDR/Flk-1 at approximately 230 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Immunocytochemistry Phospho-VEGFR2/KDR/Flk-1 (Y1214) antibody in A431 Human Cell Line by Immunocytochemistry (ICC). View Larger

Phospho-VEGFR2/KDR/Flk-1 (Y1214) in A431 Human Cell Line. VEGFR2/KDR/Flk-1 phosphorylated at Y1214 was detected in immersion fixed A431 human epithelial carcinoma cell line untreated (lower panel) or treated (upper panel) with pervanadate using Rabbit Anti-Human Phospho-VEGFR2/KDR/Flk-1 (Y1214) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1766) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; NL004) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Western Blot Detection of Human VEGFR2/KDR/Flk-1 by Western Blot View Larger

Detection of Human VEGFR2/KDR/Flk-1 by Western Blot FoxM1 and RASSF1A expression is inversely co-related in colon cancer tissues. (A) Tissue extranction from both normal and colon cancer patient for monitoring the translational level of FoxM1 and RASSF1A by immunoblot. Quantification of RASSF1A (B) and FoxM1 (C) by scanning densitometry. GAPDH was used as a loading control. (D) T84 and Colo 205 cells were treated with or without AGP IC50 (45 µM) for 48 h. Cell lysates were analyzed by Western blot for FoxM1 and GAPDH expression. (E) Quantitative estimations of FoxM1 levels determined by densitometry measurements of western blots from three independent experiments after normalization with GAPDH (p < 0.001). T84 and Colo 205 cells were treated with or without AGP as indicated before and the transcriptional level were determined by qRT-PCR for (F) FoxM1 and (G) PTTG1. Bar graphs show quantitative results normalized to GAPDH mRNA levels. Results are from three independent experiments and statistical significance was determined using one way-ANOVA followed Bonferroni test. (* p < 0.05, ** p < 0.01, *** p < 0.001). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30744076), licensed under a CC-BY license. Not internally tested by R&D Systems.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: VEGFR2/KDR/Flk-1

VEGFR2 (KDR/Flk-1), VEGFR1 (Flt-1) and VEGFR3 (Flt-4) belong to the class III subfamily of receptor tyrosine kinases (RTKs). All three receptors contain seven immunoglobulin-like repeats in their extracellular domains and kinase insert domains in their intracellular regions. The expression of VEGFR1, 2, and 3 is almost exclusively restricted to the endothelial cells. These receptors are likely to play essential roles in vasculogenesis and angiogenesis.

VEGFR2 cDNA encodes a 1356 amino acid (aa) residue precursor protein with a 19 aa residue signal peptide. Mature VEGFR2 is composed of a 745 aa residue extracellular domain, a 25 aa residue transmembrane domain and a 567 aa residue cytoplasmic domain. In contrast to VEGFR1 which binds both PlGF and VEGF with high affinity, VEGFR2 binds VEGF but not PlGF with high affinity. The recombinant soluble VEGFR2/Fc chimera binds VEGF with high affinity and is a potent VEGF antagonist.

References
  1. Ferra, N. and R. Davis-Smyth (1997) Endocrine Reviews 18:4.
Long Name
Vascular Endothelial Growth Factor Receptor 2
Entrez Gene IDs
3791 (Human); 16542 (Mouse)
Alternate Names
CD309 antigen; CD309; EC 2.7.10; EC 2.7.10.1; Fetal liver kinase 1; fetal liver kinase-1; Flk1; Flk-1; FLK1tyrosine kinase growth factor receptor; KDR; kinase insert domain receptor (a type III receptor tyrosine kinase); Kinase insert domain receptor; KRD1; Ly73; Protein-tyrosine kinase receptor flk-1; soluble VEGFR2; vascular endothelial growth factor receptor 2; VEGF R2; VEGFR; VEGFR2; VEGFR-2

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Citations for Human Phospho-VEGFR2/KDR/Flk-1 (Y1214) Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

13 Citations: Showing 1 - 10
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  1. SALM4 regulates angiogenic functions in endothelial cells through VEGFR2 phosphorylation at Tyr1175
    Authors: Dong Young Kim, Jeong Ae Park, Yeomyung Kim, Minyoung Noh, Songyi Park, Eunkyung Lie et al.
    The FASEB Journal
  2. VEGF-A isoforms program differential VEGFR2 signal transduction, trafficking and proteolysis
    Authors: Gareth W. Fearnley, Gina A. Smith, Izma Abdul-Zani, Nadira Yuldasheva, Nadeem A. Mughal, Shervanthi Homer-Vanniasinkam et al.
    Biology Open
  3. Critical role of mitogen-inducible gene 6 in restraining endothelial cell permeability to maintain vascular homeostasis
    Authors: Liying Xing, Guanqun Huang, Rongyuan Chen, Lijuan Huang, Juanxi Liu, Xiangrong Ren et al.
    Journal of Cell Communication and Signaling
  4. Endocan Knockdown Down-Regulates the Expression of Angiogenesis-Associated Genes in Il-1� Activated Chondrocytes
    Authors: Scuruchi, M;Aliqu�, F;Avenoso, A;Mandraffino, G;Vermiglio, G;Minuti, A;Campo, S;Campo, GM;D'Ascola, A;
    Biomolecules
    Species: Human
    Sample Types: Transfected Whole Cells
    Applications: Immunocytochemistry
  5. Upregulation of RASSF1A in Colon Cancer by Suppression of Angiogenesis Signaling and Akt Activation
    Authors: TG Blanchard, R Lapidus, V Banerjee, AC Bafford, SJ Czinn, H Ahmed, A Banerjee
    Cell. Physiol. Biochem., 2018-07-25;48(3):1259-1273.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  6. Endothelial progenitor cells transplantation attenuated blood-brain barrier damage after ischemia in diabetic mice via HIF-1?
    Authors: J Geng, L Wang, M Qu, Y Song, X Lin, Y Chen, M Mamtilahun, S Chen, Z Zhang, Y Wang, GY Yang
    Stem Cell Res Ther, 2017-07-11;8(1):163.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  7. DSGOST inhibits tumor growth by blocking VEGF/VEGFR2-activated angiogenesis
    Oncotarget, 2016-04-19;7(16):21775-85.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  8. Fluorescent resonance energy transfer imaging of VEGFR dimerization.
    Authors: Ahmadova Z, Yagublu V, Forg T, Hajiyeva Y, Jesenofsky R, Hafner M, Keese M
    Anticancer Res, 2014-05-01;34(5):2123-33.
    Species: Hamster
    Sample Types: Whole Cells
    Applications: ICC
  9. Endothelial cell adhesion to soluble vascular endothelial growth factor receptor-1 triggers a cell dynamic and angiogenic phenotype.
    Authors: Orecchia A, Mettouchi A, Uva P, Simon G, Arcelli D, Avitabile S, Ragone G, Meneguzzi G, Pfenninger K, Zambruno G, Failla C
    FASEB J, 2013-10-30;28(2):692-704.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  10. Vascular endothelial growth factor signals through platelet-derived growth factor receptor beta in meningiomas in vitro.
    Br. J. Cancer, 2012-10-09;107(10):1702-13.
    Species: Human
    Sample Types: Cell Lysates
    Applications: TaqMan Protein Assay
  11. Identification of Cross Talk between FoxM1 and RASSF1A as a Therapeutic Target of Colon Cancer
    Authors: Thomas G. Blanchard, Steven J. Czinn, Vivekjyoti Banerjee, Neha Sharda, Andrea C. Bafford, Fahad Mubariz et al.
    Cancers (Basel)
  12. Secretome of endothelial progenitor cells from stroke patients promotes endothelial barrier tightness and protects against hypoxia-induced vascular leakage
    Authors: Rodrigo Azevedo Loiola, Miguel García-Gabilondo, Alba Grayston, Paulina Bugno, Agnieszka Kowalska, Sophie Duban-Deweer et al.
    Stem Cell Research & Therapy
  13. Ionizing radiation increases the endothelial permeability and the transendothelial migration of tumor cells through ADAM10-activation and subsequent degradation of VE-cadherin
    Authors: PN Kouam, GA Rezniczek, IA Adamietz, H Bühler
    BMC Cancer, 2019-10-16;19(1):958.

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