Human Phospho-ZAP70 (Y493) Antibody

Catalog #: MAB7694
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB7694
MAB7694-SP

Detection of Human Phospho-ZAP70 (Y493) by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Phospho-ZAP70 (Y493) Antibody Summary

Species Reactivity

Human

Specificity

Detects human ZAP70 when phosphorylated at Y493.

Source

Monoclonal Mouse IgG_2A Clone # 797917

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Phosphopeptide containing the human ZAP70 Y493 site

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human Phospho-ZAP70 (Y493) by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line untreated (-) or treated (+) with 1 mM Pervanadate (PV) for 10 minutes or 2 mM H₂O₂for 2 minutes. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Phospho-ZAP70 (Y493) Monoclonal Antibody (Catalog # MAB7694) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Phospho-ZAP70 (Y493) at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ZAP70

ZAP70 (zeta-chain (TCR) associated protein kinase 70 kDa), expressed primarily in T and NK cells, is a Syk family cytosolic protein tyrosine kinase that consists of two N-terminal SH2 domains and a C-terminal tyrosine kinase domain. Upon T cell receptor activation and phosphorylation of TCR ITAMs by Src family kinases, ZAP70 is recruited to phosphorylated ITAM sequences and subsequently phosphorylated on several tyrosine residues. ZAP70 has been implicated in several immune disorders. An autosomal recessive form of SCID in humans has been attributed to a homozygous mutation in the kinase domain of ZAP70. ZAP70 expression also defines an aggressive subset of CLL.

Long Name

Zeta-chain [TCR] Associated Protein Kinase 70 kDa

Entrez Gene IDs

7535 (Human); 22637 (Mouse); 301348 (Rat)

Alternate Names

70 kDa zeta-associated protein; EC 2.7.10; EC 2.7.10.2; mrtle; mur; SRK; SRKFLJ17679; STD; STDFLJ17670; Syk-related tyrosine kinase; tyrosine-protein kinase ZAP-70; TZK; ZAP70; ZAP-70; zeta-chain (TCR) associated protein kinase (70 kD); zeta-chain (TCR) associated protein kinase 70kDa; zeta-chain associated protein kinase, 70kD

Product Datasheets

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Related Research Areas

Citation for Human Phospho-ZAP70 (Y493) Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

The Ciliary Machinery Is Repurposed for T Cell Immune Synapse Trafficking of LCK
Authors: Louise A. Stephen, Yasmin ElMaghloob, Michael J. McIlwraith, Tamas Yelland, Patricia Castro Sanchez, Pedro Roda-Navarro et al.
Developmental Cell