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Human Prolactin Quantikine ELISA Kit

Catalog #: DPRL00
5 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
DPRL00
Control Products Available
QC149: Quantikine Immunoassay Control Set 766 for Human Prolactin
Human Prolactin ELISA Standard Curve
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Product Details
Procedure
Citations (5)
FAQs
Supplemental Products
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Summary Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Human Prolactin Quantikine ELISA Kit Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL)
Sensitivity
0.264 ng/mL
Assay Range
1.6 - 100 ng/mL (Serum, EDTA Plasma, Heparin Plasma)
Specificity
Natural and recombinant human Prolactin and natural human macroprolactin
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
Interference observed with 1 or more available related molecules.

Product Summary

The Quantikine Human Prolactin Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human Prolactin in serum and plasma. It contains E. coli-expressed recombinant human Prolactin and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human Prolactin showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring Prolactin.

Recovery

The recovery of Prolactin spiked to levels throughout the range of the assay was evaluated.

Sample Type Average % Recovery Range %
EDTA Plasma (n=4) 94 84-105
Heparin Plasma (n=4) 97 90-104
Serum (n=4) 94 84-108

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Prolactin were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human Prolactin ELISA Linearity

Scientific Data

N/A Human Prolactin ELISA Standard Curve View Larger

Human Prolactin ELISA Standard Curve

Product Datasheets

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Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Prolactin

Prolactin (PRL) is a neuroendocrine pituitary hormone that is synthesized by anterior pituitary, placenta, brain, uterus, dermal fibroblasts, decidua, B cells, T cells, natural killer cells, and breast cancer cells. The prolactin receptor is a transmembrane type I glycoprotein that belongs to the hematopoietic cytokine receptor family.

Prolactin receptor (PRL R) contains an extracellular, transmembrane, and intracellular domain. Transcriptional regulation of the PRL R gene results in several different species-dependent isoforms of PRL R being produced.

Entrez Gene IDs:
5617 (Human); 19109 (Mouse)
Alternate Names:
PRL; Prolactin
&#9888; WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Assay Procedure

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
    3. 100 µL Assay Diluent
  3.   Add 100 µL of Assay Diluent to each well.
    4. 50 µL Standard, Control, or Sample
  4.   Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
  5.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.
    6. 200 µL Conjugate
  6.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
  7.   Aspirate and wash 4 times.
    8. 200 µL Substrate Solution
  8.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.
    9. 50 µL Stop Solution
  9.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Citations for Human Prolactin Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Development of cell-laden multimodular Lego-like customizable endometrial tissue assembly for successful tissue regeneration
    Authors: SR Park, MG Kook, SR Kim, JW Lee, CH Park, BC Oh, Y Jung, IS Hong
    Biomaterials research, 2023-04-21;27(1):33.
    Species: Human
    Sample Types: Cell Culture Supernates
  2. Subtle changes in perivascular endometrial mesenchymal stem cells after local endometrial injury in recurrent implantation failure
    Authors: Y Fan, RWK Lee, XW Ng, CE Gargett, JKY Chan
    Scientific Reports, 2023-01-05;13(1):225.
    Species: Human
    Sample Types: Cell Culture Supernates
  3. Bromocriptine-QR Therapy Reduces Sympathetic Tone and Ameliorates a Pro-Oxidative/Pro-Inflammatory Phenotype in Peripheral Blood Mononuclear Cells and Plasma of Type 2 Diabetes Subjects
    Authors: AH Cincotta, E Cersosimo, M Alatrach, M Ezrokhi, C Agyin, J Adams, R Chilton, C Triplitt, B Chamarthi, N Cominos, RA DeFronzo
    International Journal of Molecular Sciences, 2022-08-09;23(16):.
    Species: Human
    Sample Types: Plasma
  4. Hormonal determinants of mammographic density and density change
    Authors: M Gabrielson, S Azam, E Hardell, M Holm, KA Ubhayaseke, M Eriksson, M Bäcklund, J Bergquist, K Czene, P Hall
    Breast Cancer Res., 2020-08-26;22(1):95.
    Species: Human
    Sample Types: Plasma
  5. First-in-human Clinical Trial of Oral ONC201 in Patients with Refractory Solid Tumors
    Authors: MN Stein, JR Bertino, HL Kaufman, T Mayer, R Moss, A Silk, N Chan, J Malhotra, L Rodriguez-, J Aisner, RD Aiken, BG Haffty, RS DiPaola, T Saunders, A Zloza, S Damare, Y Beckett, B Yu, S Najmi, C Gabel, S Dickerson, L Zheng, WS El-Deiry, JE Allen, M Stogniew, W Oster, JM Mehnert
    Clin. Cancer Res, 2017-03-22;0(0):.
    Species: Human
    Sample Types: Serum

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