Human RARRES3 Antibody

Catalog #: MAB6139 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB6139
MAB6139-SP

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human RARRES3 Antibody Summary

Species Reactivity

Human

Specificity

Recognizes human RARRES3 in Western blots and direct ELISAs. In Western blots and direct ELISAs, no cross-reactivity with recombinant human (rh) RARRES1 or rhLatexin is observed.

Source

Monoclonal Mouse IgG_2B Clone # 659209

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human RARRES3
Asp12-Lys134
Accession # Q9UL19

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Immunocytochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry

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RARRES3 in Raji Human Cell Line. RARRES3 was detected in immersion fixed Raji human Burkitt's lymphoma cell line using Mouse Anti-Human RARRES3 Monoclonal Antibody (Catalog # MAB6139) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: RARRES3

RARRES3 (retinoic acid receptor responder 3), also called Tig3 (tazarotene-induced gene 3) or RIG1 (retinoid-inducible gene 1), is an 18 kDa, 164 amino acid (aa) cytoplasmic protein within the LRAT (lecithin retinol acyltransferase) family of growth inhibitory proteins, which are class II tumor-suppressors. RARRES3 is expressed in a variety of cells including epidermal keratinocytes, and may be downregulated in tumor cells and cell lines. It shows phospholipase activity and interaction with type I transglutaminase. A C-terminal hydrophobic region allows perinuclear localization. RARRES3 is not structurally related to other RARRES genes. A canine ortholog shares 72% aa identity within the region used as an immunogen; orthologs are unlikely in rodents.

Long Name

Retinoic Acid Receptor Responder [Tazarotene-induced] 3

Entrez Gene IDs

5920 (Human)

Alternate Names

HRASLS4; PLA1/2-3; RARRES3; RAR-responsive protein TIG3; retinoic acid receptor responder (tazarotene induced) 3; retinoic acid receptor responder protein 3; retinoic acid-inducible gene 1; Retinoid-inducible gene 1 protein; RIG1; Tazarotene-induced gene 3 protein; TIG3; TIG3MGC8906