Human Ras Antibody Summary
Met1-Met188 (Gln61His)
Accession # P01116-2
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Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human Ras by Western Blot. Western blot shows lysates of K562 human chronic myelo-genous leukemia cell line and Ramos human Burkitt's lymphoma cell line. PVDF membrane was probed with 5 µg/mL Rat Anti-Human Ras Monoclonal Antibody (Catalog # MAB3429) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). For additional refer-ence, recombinant human H-Ras, N Ras, K-Ras2A and 2B, R-Ras2 and 3, Rap2A and 2B, RalA and B, and Rheb (5 ng/lane) were included. A specific band for Ras was detected at approximately 21 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Ras
The Ras proteins (H-Ras, N-Ras, and two splice variants of K-Ras) define a closely related family of 21 kDa GTPases that cycle between GTP-bound active and GDP-bound inactive states at the cytoplasmic face of the plasma membrane. Receptor tyrosine kinases and G-protein coupled receptors activate Ras, and effectors include Raf and PI 3-kinase. Activating mutations of Ras, which impair GTPase activity and stabilize the active state, are found in nearly one-third of all human cancers.
Product Datasheets
Citation for Human Ras Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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TRPC3 shapes the ER-mitochondria Ca2+ transfer characterizing tumour-promoting senescence
Authors: V Farfariell, DV Gordienko, L Mesilmany, Y Touil, E Germain, I Fliniaux, E Desruelles, D Gkika, M Roudbaraki, G Shapovalov, L Noyer, M Lebas, L Allart, N Zienthal-G, O Iamshanova, F Bonardi, M Figeac, W Laine, J Kluza, P Marchetti, B Quesnel, D Metzger, D Bernard, JB Parys, L Lemonnier, N Prevarskay
Nature Communications, 2022-02-17;13(1):956.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
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