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Human Resistin DuoSet ELISA

Catalog #: DY1359
22 Citations 2 Reviews Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
DY1359
Ancillary Products Available
DY008B: DuoSet ELISA Ancillary Reagent Kit 2
DY999B: Substrate Reagent Pack
DY999: Substrate Reagent Pack
Human Resistin ELISA Standard Curve
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Product Details
Procedure
Citations (22)
FAQs
Supplemental Products
Reviews (2)
Summary Product Features Kit Content Other Reagents Required Data Examples Product Datasheets Preparation and Storage Background

Human Resistin DuoSet ELISA Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Volume Required
100 µL
Assay Range
31.2 - 2,000 pg/mL
Sufficient Materials
For fifteen 96-well plates*
Specificity
Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human Resistin. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet.

 

Product Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Economical alternative to complete kits

Kit Content

  • Capture Antibody
  • Detection Antibody
  • Recombinant Standard
  • Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

DuoSet Ancillary Reagent Kit 2 (5 plates): (Catalog # DY008) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 2.

The components listed above may be purchased separately:

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or 0.05% Tween® 20 in PBS, pH 7.2-7.4

Reagent Diluent: (Catalog # DY995), or 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990)

Plate Sealers: ELISA Plate Sealers (Catalog # DY992)

Scientific Data

N/A Human Resistin ELISA Standard Curve View Larger

Human Resistin ELISA Standard Curve

Product Datasheets

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Product Datasheet
COA
SDS

Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Resistin

Resistin, also known ADSF and FIZZ3, is a secreted peptide hormone that circulates as either a noncovalent trimer, or a disulfide-linked homohexamer. Resistin is expressed by white adipocytes, splenocytes, astrocytes, and anterior pituitary epithelium. It blocks Insulin-stimulated uptake of glucose by adipocytes and promotes glucose release by hepatocytes. High circulating Insulin levels induce Resistin secretion which then antagonize Insulin action.

Entrez Gene IDs:
56729 (Human); 57264 (Mouse)
Alternate Names:
Adipose tissue-specific secretory factor; ADSF; ADSFMGC126609; C/EBP-epsilon regulated myeloid-specific secreted cysteine-rich proteinprecursor 1; C/EBP-epsilon-regulated myeloid-specific secreted cysteine-rich protein; Cysteine-rich secreted protein A12-alpha-like 2; Cysteine-rich secreted protein FIZZ3; FIZZ3; FIZZ3MGC126603; found in inflammatory zone 3; HXCP1; Resistin; RETN; RETN1; RSTNXCP1

Assay Procedure

GENERAL ELISA PROTOCOL

Plate Preparation

  1. Dilute the Capture Antibody to the working concentration in PBS without carrier protein. Immediately coat a 96-well microplate with 100 μL per well of the diluted Capture Antibody. Seal the plate and incubate overnight at room temperature.
  2. Aspirate each well and wash with Wash Buffer, repeating the process two times for a total of three washes. Wash by filling each well with Wash Buffer (400 μL) using a squirt bottle, manifold dispenser, or autowasher. Complete removal of liquid at each step is essential for good performance. After the last wash, remove any remaining Wash Buffer by aspirating or by inverting the plate and blotting it against clean paper towels.
  3. Block plates by adding 300 μL Reagent Diluent to each well. Incubate at room temperature for a minimum of 1 hour.
  4. Repeat the aspiration/wash as in step 2. The plates are now ready for sample addition.

Assay Procedure

  1. Add 100 μL of sample or standards in Reagent Diluent, or an appropriate diluent, per well. Cover with an adhesive strip and incubate 2 hours at room temperature.
  2. Repeat the aspiration/wash as in step 2 of Plate Preparation.
  3. Add 100 μL of the Detection Antibody, diluted in Reagent Diluent, to each well. Cover with a new adhesive strip and incubate 2 hours at room temperature.
  4. Repeat the aspiration/wash as in step 2 of Plate Preparation.
  5. Add 100 μL of the working dilution of Streptavidin-HRP to each well. Cover the plate and incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
  6. Repeat the aspiration/wash as in step 2.
  7. Add 100 μL of Substrate Solution to each well. Incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
  8. Add 50 μL of Stop Solution to each well. Gently tap the plate to ensure thorough mixing.
  9. Determine the optical density of each well immediately, using a microplate reader set to 450 nm. If wavelength correction is available, set to 540 nm or 570 nm. If wavelength correction is not available, subtract readings at 540 nm or 570 nm from the readings at 450 nm. This subtraction will correct for optical imperfections in the plate. Readings made directly at 450 nm without correction may be higher and less accurate.

Citations for Human Resistin DuoSet ELISA

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

22 Citations: Showing 1 - 10
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  1. Cognitive function is associated with performance in time up and go test and with leptin blood levels in community-dwelling older women
    Authors: da Costa Teixeira, LA;Soares, LA;Lima, LP;Avelar, NCP;de Moura, JA;Leopoldino, AAO;Figueiredo, PHS;Parentoni, AN;Mendonça, VA;Lacerda, ACR;
    Scientific reports
    Species: Human
    Sample Types: Plasma
  2. Using the Traditional Ex Vivo Whole Blood Model to Discriminate Bacteria by Their Inducible Host Responses
    Authors: Chick, HM;Rees, ME;Lewis, ML;Williams, LK;Bodger, O;Harris, LG;Rushton, S;Wilkinson, TS;
    Biomedicines
    Species: Human
    Sample Types: Whole Blood
  3. The strong inverse association between plasma concentrations of soluble tumor necrosis factor receptors type 1 with adiponectin/leptin ratio in older women
    Authors: Augusto da Costa Teixeira, L;Rocha-Vieira, E;Aparecida Soares, L;Mota de Oliveira, F;Aparecida Oliveira Leopoldino, A;Netto Parentoni, A;Amaral Mendonça, V;Cristina Rodrigues Lacerda, A;
    Cytokine
    Species: Human
    Sample Types: Plasma
  4. Inflammatory biomarkers at different stages of Sarcopenia in older women
    Authors: da Costa Teixeira, LA;Avelar, NCP;Peixoto, MFD;Parentoni, AN;Santos, JMD;Pereira, FSM;Danielewicz, AL;Leopoldino, AAO;Costa, SP;Arrieiro, AN;Soares, LA;da Silva Lage, VK;Prates, ACN;Taiar, R;de Carvalho Bastone, A;Oliveira, VC;Oliveira, MX;Costa, HS;Nobre, JNP;Brant, FP;Duarte, TC;Figueiredo, PHS;Mendonça, VA;Lacerda, ACR;
    Scientific reports
    Species: Human
    Sample Types: Plasma
  5. Adiponectin Is a Contributing Factor of Low Appendicular Lean Mass in Older Community-Dwelling Women: A Cross-Sectional Study
    Authors: LAC Teixeira, JM Dos Santos, AN Parentoni, LP Lima, TC Duarte, FP Brant, CDC Neves, FSM Pereira, NCP Avelar, AL Danielewic, AAO Leopoldino, SP Costa, AN Arrieiro, LA Soares, ACN Prates, JNP Nobre, A de Carvalh, VC de Oliveir, MX Oliveira, PH Scheidt Fi, HS Costa, V Amaral Men, R Taiar, AC Rodrigues
    Journal of Clinical Medicine, 2022-12-02;11(23):.
    Species: Human
    Sample Types: Plasma
  6. No association between intravenous fluid volume and endothelial glycocalyx shedding in patients undergoing resuscitation for sepsis in the emergency department
    Authors: S Macdonald, E Bosio, NI Shapiro, L Balmer, S Burrows, M Hibbs, T Jowitt, L Smart, G Arendts, D Fatovich
    Scientific Reports, 2022-05-24;12(1):8733.
    Species: Human
    Sample Types: Serum
  7. Prediction of emergency cerclage outcomes in women with cervical insufficiency: The role of inflammatory, angiogenic, and extracellular matrix-related proteins in amniotic fluid
    Authors: KN Lee, KH Park, YM Kim, I Cho, TE Kim
    PLoS ONE, 2022-05-10;17(5):e0268291.
    Species: Human
    Sample Types: Amniotic Fluid
  8. Gestational Weight Gain Influences the Adipokine-Oxidative Stress Association during Pregnancy
    Authors: JM Solis Pare, O Perichart, A Montoya Es, E Reyes Muño, S Espino Y S, V Ortega Cas, D Medina Bas, M Tolentino, M Sanchez Ma, S Nava Salaz, G Estrada Gu
    Obesity facts, 2021-09-14;0(0):1-9.
    Species: Human
    Sample Types: Serum
  9. The dynamics of human bone marrow adipose tissue in response to feeding and fasting
    Authors: PK Fazeli, MA Bredella, OG Pachon-Peñ, W Zhao, X Zhang, AT Faje, M Resulaj, SP Polineni, TM Holmes, H Lee, EK O'Donnell, OA MacDougald, MC Horowitz, CJ Rosen, A Klibanski
    JCI Insight, 2021-06-22;0(0):.
    Species: Human
    Sample Types: Serum
  10. Impact of the Uncoupling Protein 1 on Cardiovascular Risk in Patients with Rheumatoid Arthritis
    Authors: LI Lyngfelt, MC Erlandsson, M Nadali, S Hedjazifar, R Pullerits, KM Andersson, P Brembeck, ST Silfverswä, U Smith, MI Bokarewa
    Cells, 2021-05-07;10(5):.
    Species: Human
    Sample Types: Serum
  11. The effect of Gestational Diabetes Mellitus on the fetal compartment
    Authors: TPB De Luccia, E Ono, R Menon, AU Borbely, R Mattar, L Richardson, ALM da Silva, RM Botelho, MLTLF da Rocha, S Daher
    Journal of reproductive immunology, 2021-03-24;145(0):103314.
    Species: Human
    Sample Types: Serum
  12. Reduced concentrations of the B cell cytokine Interleukin 38 are associated with cardiovascular disease risk in overweight subjects
    Authors: DM de Graaf, M Jaeger, ICL van den Mu, RT Horst, K Schraa, J Zwaag, M Kox, M Fujita, T Yamauchi, L Mercurio, S Madonna, JHW Rutten, J de Graaf, NP Riksen, FL van de Vee, MG Netea, LAB Joosten, CA Dinarello
    Eur J Immunol, 2020-11-19;0(0):.
    Species: Human
    Sample Types: Plasma
  13. BCG vaccination in humans inhibits systemic inflammation in a sex-dependent manner
    Authors: VACM Koeken, LCJ de Bree, VP Mourits, SJ Moorlag, J Walk, B Cirovic, RJ Arts, M Jaeger, H Dijkstra, H Lemmers, LA Joosten, CS Benn, R van Crevel, M Netea
    J. Clin. Invest., 2020-10-01;0(0):.
    Species: Human
    Sample Types: Plasma
  14. Elevation of C-reactive protein, P-selectin and Resistin as potential inflammatory biomarkers of urogenital Schistosomiasis exposure in preschool children
    Authors: TN Chimponda, C Mushayi, DNM Osakunor, A Vengesai, E Enwono, S Amanfo, J Murray, C Tshuma, F Mutapi, T Mduluza
    BMC Infect. Dis., 2019-12-19;19(1):1071.
    Species: Human
    Sample Types: Serum
  15. Effects of the inspiratory muscle training and aerobic training on respiratory and functional parameters, inflammatory biomarkers, redox status and quality of life in hemodialysis patients: A randomized clinical trial
    Authors: PHS Figueiredo, MMO Lima, HS Costa, JB Martins, OD Flecha, PF Gonçalves, FL Alves, VGB Rodrigues, EHB Maciel, VA Mendonça, ACR Lacerda, ÉLM Vieira, AL Teixeira, F de Paula, CH Balthazar
    PLoS ONE, 2018-07-26;13(7):e0200727.
    Species: Human
    Sample Types: Plasma
  16. Association between Resistin Gene Polymorphisms and Atopic Dermatitis
    Authors: SA Banihani, KF Abu-Alia, OF Khabour, KH Alzoubi
    Biomolecules, 2018-03-27;8(2):.
    Species: Human
    Sample Types: Serum
  17. Using Resistin, glucose, age and BMI to predict the presence of breast cancer
    Authors: M Patrício, J Pereira, J Crisóstomo, P Matafome, M Gomes, R Seiça, F Caramelo
    BMC Cancer, 2018-01-04;18(1):29.
    Species: Human
    Sample Types: Serum
  18. High Expression of STAT3 in Subcutaneous Adipose Tissue Associates with Cardiovascular Risk in Women with Rheumatoid Arthritis
    Authors: M Nadali, R Pullerits, KME Andersson, ST Silfverswä, MC Erlandsson, MI Bokarewa
    Int J Mol Sci, 2017-11-13;18(11):.
    Species: Human
    Sample Types: Serum
  19. Presence of IL-17 in synovial fluid identifies a potential inflammatory osteoarthritic phenotype
    Authors: SJ Snelling, S Bas, GJ Puskas, SG Dakin, D Suva, A Finckh, C Gabay, P Hoffmeyer, AJ Carr, A Lübbeke
    PLoS ONE, 2017-04-11;12(4):e0175109.
    Species: Human
    Sample Types: Serum
  20. Association study of arcuate nucleus neuropeptide Y neuron receptor gene variation and serum NPY levels in clozapine treated patients with schizophrenia
    Authors: E Leinonen
    Eur. Psychiatry, 2016-11-10;40(0):13-19.
    Species: Human
    Sample Types: Serum
  21. Sirt1 inhibits resistin expression in aortic stenosis.
    Authors: Carter S, Miard S, Roy-Bellavance C, Boivin L, Li Z, Pibarot P, Mathieu P, Picard F
    PLoS ONE, 2012-04-06;7(4):e35110.
    Species: Human
    Sample Types: Cell Culture Supernates
  22. Adipokine resistin predicts anti-inflammatory effect of glucocorticoids in asthma.
    Authors: Leivo-Korpela S, Lehtimaki L, Vuolteenaho K, Nieminen R, Kankaanranta H, Saarelainen S, Moilanen E
    J Inflamm (Lond), 2011-05-26;8(0):12.
    Species: Human
    Sample Types: Plasma

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Reviews for Human Resistin DuoSet ELISA

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Human Resistin DuoSet ELISA
By Balaji Mahender on 12/20/2017
Sample Tested: EDTA Plasma
Human Resistin DuoSet ELISA
By Anonymous on 08/03/2017
Sample Tested: Serum

Sample dilution used - 1:5

Human Resistin DuoSet ELISA DY1359