Human SARM1 Antibody

Catalog #: AF7037 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF7037
AF7037-SP

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Human SARM1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human SARM1 in direct ELISAs and Western blots.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human SARM1
Lys556-Thr724
Accession # Q6SZW1

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

SH‑SY5Y human neuroblastoma cell line

Immunoprecipitation

4 µg/mL

See below

Immunocytochemistry

5-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunoprecipitation

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Immunoprecipitation of Human SARM1. SARM1 was immunoprecipitated from 400 µg of SH‑SY5Y human neuroblastoma cell line lysates using 4 µg of Sheep Anti-Human SARM1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7037) coated on 4 wells of a 96 well plate (Corning Costar EIA/RIA). SH‑SY5Y lysates or control buffer were added to the wells and incubated for 2 hours at room temperature. Immunoprecipitated SARM1 was detected by Western blot under reducing conditions using 1.0 µg/mL Sheep Anti-Human SARM1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7037) and Immunoblot Buffer Group 1. View our recommended buffer recipes for Immunoprecipitation.

Immunocytochemistry

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SARM1 in HEK293 Human Cell Line. SARM1 was detected in immersion fixed HEK293 human embryonic kidney cell line using Sheep Anti-Human SARM1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7037) at 1.7 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SARM1

SARM1 (Sterile alpha and TIR motif-containing protein 1; also SAM domain-containing protein 2 and TIR-1 homolog) is a 73-75 kDa member of the TLR adaptor family of molecules. It is both nuclear and cytoplasmic, and expressed in monocytes, neurons, and retinal photoreceptor cells, where it demonstrates disparate activities. In monocytes, SARM1 is a specific inhibitor of TRIF-dependent TLR-3 and -4 signaling, and appears to block MAPK phosphorylation. In neurons, SARM1 regulates microtubule stability, and thus axon and dendrite elongation. And in retinal photoreceptor cells, SARM1 complexes with Na/K ATPase to create a cell surface receptor for retinoschisin. Human SARM1 is 724 amino acids (aa) in length. It contains an N-terminal polybasic motif (aa 1-20) and a Gly-rich region (aa 22-90) that may anchor the molecule to intracellular membranes. It also possesses two SAM domains (aa 412-548) plus a TIR region (aa 559-657) that appears to interact with TIRF. Proteolytic cleavage products of 35 kDa and 30 kDa have been described. There is one splice variant that shows a 72 aa substitution for aa 1-106. Over aa 556-724, human SARM1 shares 94% aa identity with mouse SARM1.

Long Name

Sterile alpha and TIR Motif Containing 1

Entrez Gene IDs

23098 (Human); 237868 (Mouse); 287545 (Rat)

Alternate Names

KIAA0524SAM domain-containing protein 2; SAMD2; SAMD2sterile alpha and HEAT/Armadillo motif protein, ortholog of Drosophila; SARM1; SARMFLJ36296; Sterile alpha and Armadillo repeat protein; sterile alpha and TIR motif containing 1; sterile alpha and TIR motif-containing protein 1; Sterile alpha motif domain-containing protein 2; Tir-1 homolog