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Human Serpin E1/PAI-1 Antibody

Catalog #: MAB1786
9 Citations 1 Review Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB1786-SP
MAB1786-500
MAB1786-100
Detection of Human Serpin E1/PAI‑1 by Western Blot.
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Citations (9)
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Reviews (1)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Serpin E1/PAI-1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Serpin E1 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human Serpin A1, A3, A4, A8, C1, F1, F2, I1, I2, recombinant mouse Serpin D1 or E2 is observed.
Source
Monoclonal Mouse IgG1 Clone # 242816
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Serpin E1/PAI-1
Met1-Pro402
Accession # P05121
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Simple Western
2.5 µg/mL
See below

Human Serpin E1/PAI-1 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: Human Serpin E1/PAI‑1 Biotinylated Antibody (Catalog # BAF1786)

Standard: Recombinant Human Serpin E1/PAI-1 Protein, CF (Catalog # 1786-PI)

Neutralization
Measured by its ability to neutralize Recombinant Human Serpin E1/PAI-1 (0.25 µg/mL, Catalog # 1786-PI) inhibition of Recombinant Human u‑Plasminogen Activator (uPA)/Urokinase (0.1 µg/mL, Catalog # 1310-SE) cleavage of the fluorogenic peptide substrate Z-GGR-AMC (100 µM). The Neutralization Dose (ND50) is typically 0.3 µg/mL.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human Serpin E1/PAI-1 antibody by Western Blot. View Larger

Detection of Human Serpin E1/PAI‑1 by Western Blot. Western blot shows lysates of HUVEC human umbilical vein endothelial cells. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Serpin E1/PAI-1 Monoclonal Antibody (Catalog # MAB1786) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Serpin E1/PAI-1 at approximately 48 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western Detection of Human Serpin E1/PAI-1 antibody by Simple Western<sup>TM</sup>. View Larger

Detection of Human Serpin E1/PAI‑1 by Simple WesternTM. Simple Western lane view shows lysates of HUVEC human umbilical vein endothelial cells, loaded at 0.2 mg/mL. A specific band was detected for Serpin E1/PAI‑1 at approximately 54 kDa (as indicated) using 2.5 µg/mL of Mouse Anti-Human Serpin E1/PAI‑1 Monoclonal Antibody (Catalog # MAB1786). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Neutralization Neutralization of Serpin E1/ PAI‑1 Activity by Human Serpin E1/PAI‑1 Antibody. View Larger

Neutralization of Serpin E1/ PAI‑1 Activity by Human Serpin E1/PAI‑1 Antibody. Recombinant Human u-Plasminogen Activator (uPA)/Urokinase (0.1 µg/mL, Catalog # 1310-SE) activity is measured in the presence of Recombinant Human Serpin E1/PAI-1 (0.25 µg/mL, Catalog # 1786-PI) that has been preincubated with increasing concentrations of Mouse Anti-Human Serpin E1/PAI-1 Monoclonal Antibody (Catalog # MAB1786). The ND50 is typically 0.3 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Serpin E1/PAI-1

As a member of the Serpin superfamily of serine protease inhibitors, Serpin E1/PAI‑1 is the principal inhibitor of urokinase‑type plasminogen activator (uPA) and tissue‑type PA (1, 2). As important regulators of extracellular matrix remodeling, uPA and PAI‑1 play a major role in many processes such as angiogenesis, tumor invasion and obesity (2‑4). For example, uPA and PAI-1 are the only tumor prognostic factors validated at the highest level of evidence with regard to their clinical utility in breast cancer (5). The human PAI-1 is initially synthesized as 402 amino acid precursor with a N‑terminal signal peptide (6, 7). PAI‑1 may exist in one of two possible conformations, designated as active or latent (8). The purified rhPAI‑1 is active against rhuPA. The heterogeneity at the N‑terminus of the purified recombinant human PAI‑1 has been observed before for both the recombinant and native proteins (9).

References
  1. Silverman, G.A. et al. (2001) J. Biol. Chem. 276:33293.
  2. Stefansson, S. et al. (2003) Curr. Pharm. Des. 9:1545.
  3. Duffy, M.J. (2002) Clin. Chem. 48:1194.
  4. Juhan-Vague, I. et al. (2003) J. Thromb. Haemost. 1:1575.
  5. Harbeck, N. et al. (2002) Clin. Breast Cancer 3:196.
  6. Pannekoek, H. et al. (1986) EMBO J. 5:2539.
  7. Ginsburg, D. et al. (1986) J. Clin. Invest. 78:1673.
  8. Wang, Z. et al. (1996) Biochemistry 35:16443.
  9. Stromqvist, M. et al. (1994) Protein Expr. Purif. 5:309.
Long Name
Plasminogen Activator Inhibitor
Entrez Gene IDs
5054 (Human); 18787 (Mouse)
Alternate Names
Endothelial plasminogen activator inhibitor; Nexin; PAI1; PAI-1; PAI1PAI-1; PAISerpin E1; PLANH1; PLANH1plasminogen activator inhibitor 1; serine (or cysteine) proteinase inhibitor, clade E (nexin, plasminogenactivator inhibitor type 1), member 1; Serpin E1; serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitortype 1), member 1

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Citations for Human Serpin E1/PAI-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
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  1. Senolytics reduce coronavirus-related mortality in old mice
    Authors: Christina D. Camell, Matthew J. Yousefzadeh, Yi Zhu, Larissa G. P. Langhi Prata, Matthew A. Huggins, Mark Pierson et al.
    Science
  2. Angiogenic Potential of Tissue Engineered Cartilage From Human Mesenchymal Stem Cells Is Modulated by Indian Hedgehog and Serpin E1
    Authors: Yannick Nossin, Eric Farrell, Wendy J. L. M. Koevoet, Rodrigo A. Somoza, Arnold I. Caplan, Bent Brachvogel et al.
    Frontiers in Bioengineering and Biotechnology
  3. Orally-active, clinically-translatable senolytics restore alpha-Klotho in mice and humans
    Authors: Y Zhu, LGPL Prata, EOW Gerdes, JME Netto, T Pirtskhala, N Giorgadze, U Tripathi, CL Inman, KO Johnson, A Xue, AK Palmer, T Chen, K Schaefer, JN Justice, AM Nambiar, N Musi, SB Kritchevsk, J Chen, S Khosla, D Jurk, MJ Schafer, T Tchkonia, JL Kirkland
    EBioMedicine, 2022-03-13;0(0):103912.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  4. Senolytics reduce coronavirus-related mortality in old mice
    Authors: Christina D. Camell, Matthew J. Yousefzadeh, Yi Zhu, Larissa G. P. Langhi Prata, Matthew A. Huggins, Mark Pierson et al.
    Science
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  5. Single-cell transcriptional changes associated with drug tolerance and response to combination therapies in cancer
    Authors: AF Aissa, ABMMK Islam, MM Ariss, CC Go, AE Rader, RD Conrardy, AM Gajda, C Rubio-Pere, K Valyi-Nagy, M Pasquinell, LE Feldman, SJ Green, N Lopez-Biga, MV Frolov, EV Benevolens
    Nature Communications, 2021-03-12;12(1):1628.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Cell Lysates
  6. SILAC Analysis Reveals Increased Secretion of Hemostasis-Related Factors by Senescent Cells
    Authors: CD Wiley, S Liu, C Limbad, AM Zawadzka, J Beck, M Demaria, R Artwood, F Alimirah, JA Lopez-Domi, C Kuehnemann, SR Danielson, N Basisty, HG Kasler, TR Oron, PY Desprez, SD Mooney, BW Gibson, B Schilling, J Campisi, P Kapahi
    Cell Rep, 2019-09-24;28(13):3329-3337.e5.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  7. A multiplex immunoassay for human adipokine profiling.
    Authors: Schipper HS, De Jager W, van Dijk ME, Meerding J, Zelissen PM, Adan RA, Prakken BJ, Kalkhoven E
    Clin. Chem., 2010-06-08;56(0):1320.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Luminex Development
  8. Human CD4- 8- T cells are a distinctive immunoregulatory subset.
    Authors: Huang MC, Patel K, Taub DD, Longo DL, Goetzl EJ
    FASEB J, 2010-02-12;24(7):2558-66.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  9. Role of Corneal Stromal Cells on Epithelial Cell Function during Wound Healing
    Authors: Bhavani S. Kowtharapu, Radovan Murín, Anselm G. M. Jünemann, Oliver Stachs
    International Journal of Molecular Sciences

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Human Serpin E1/PAI-1 Antibody
By Prasanna Ekambaram on 05/14/2018
Application: B/N Sample Tested: Breast cancer cells Species: Human

We used this Serpin E1 neutralizing antibody (400 ng/mL) as part of an antibody cocktail containing several other antibodies to neutralize/block Serpin E1 present in the Conditioned Medium (CM) from BT549 Cells treated with Angiotensin II.

CM (+/- Serpin E1 Neutralizing ab Cocktail) was used to further look at Endothelial (HUVEC) Cell migration using Incucyte Chemotaxis Assay