Human Siglec-10 Antibody

Name: Human Siglec-10 Antibody
Brand: R&D Systems
SKU: MAB21301

Catalog #: MAB21301 Datasheet / COA / SDS

Discontinued Product

MAB21301 has been discontinued.

View all Siglec-10 products.

Detection of Siglec‑10 in Human CD19+B Cells by Flow Cytometry.

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All Siglec-10 Antibodies

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Human Siglec-10 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Siglec-10 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human Siglec-2, -3, -5, -6, -7, -9, -11 or recombinant mouse Siglec-F is observed.

Source

Monoclonal Mouse IgG₁ Clone # 265821

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Siglec-10
Met17-Thr546
Accession # Q96LC7

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Applications

Recommended Concentration

Sample

Flow Cytometry

2.5 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of Siglec‑10 in Human CD19⁺B Cells by Flow Cytometry. Human blood-derived lymphocytes were stained with Mouse Anti-Human Siglec-10 Monoclonal Antibody (Catalog # MAB21301) followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')₂Secondary Antibody (Catalog # F0101B) and Human CD19 Phycoerythrin-conjugated Monoclonal Antibody (Catalog # FAB4867P).Quadrant markers were set based on control antibody staining (Catalog # MAB002).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Siglec-10

Siglecs (sialic acid binding Ig-like lectins) are I-type lectins that belong to the immunoglobulin superfamily. They are characterized by an N‑terminal Ig-like V-type domain which mediates sialic acid binding, followed by a varying number of Ig-like C2-type domains. Siglecs 5‑11 constitute the CD33/Siglec-3 related group, and are differentially expressed in the hematopoietic system (1‑3). Siglec-G is the apparent ortholog of human Siglec-10 (4). The human Siglec-10 cDNA encodes a 697 amino acid (aa) precursor that includes a 16 aa signal sequence, a 534 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 126 aa cytoplasmic domain. The ECD contains one Ig-like V-type domain and four Ig-like C2-type domains, while the cytoplasmic domain contains two immunoreceptor tyrosine-based inhibitory motifs (ITIM) (5‑8). Five splice variants of human Siglec-10 differ in their deletions within the ECD. A potentially secreted sixth variant contains the Ig-like V-type domain followed by a 45 aa substitution (5‑7, 9). Within the ECD, human Siglec-10 is most closely related to Siglec-5 (42% aa sequence identity). It shares 63% aa sequence identity with mouse Siglec-G. Siglec-10 is expressed on eosinophils, neutrophils, monocytes, and B cells (5, 8) with some splice variants predominating in particular cell types and tissue locations (6, 7, 9). It is up‑regulated on eosinophils in mouse models of allergic respiratory inflammation (10). Siglec‑10 binds sialated proteins and lipids in alpha 2,3 or alpha 2,6 linkage and shows a preference for GT1b gangliosides (7, 11). This binding can be modulated by cis interactions of Siglec-10 with sialated molecules expressed on the same cell (7). When tyrosine phosphorylated, the cytoplasmic ITIMs interact with phosphatases SHP-1 and SHP‑2 to propagate inhibitory signals (5, 9).

References

Crocker, P.R. (2005) Curr. Opin. Pharmacol. 5:431.
Crocker, P.R. (2002) Curr. Opin. Struct. Biol. 12:609.
Crocker, P.R. and J. Zhang (2002) Biochem. Soc. Symp. 69:83.
Angata, T. et al. (2001) J. Biol. Chem. 276:45128.
Whitney, G. et al. (2001) Eur. J. Biochem. 268:6083.
Yousef, G.M. et al. (2001) Biochem. Biophys. Res. Commun. 284:900.
Li, N. et al. (2001) J. Biol. Chem. 276:28106.
Munday, J. et al. (2001) Biochem. J. 355:489.
Kitzig, F. et al. (2002) Biochem. Biophys. Res. Commun. 296:355.
Aizawa, H. et al. (2003) Genomics 82:521.
Rapoport, E. et al. (2003) Bioorg. Med. Chem. Lett. 13:675.

Long Name

Sialic Acid Binding Ig-like Lectin 10

Entrez Gene IDs

89790 (Human)

Alternate Names

MGC126774; PRO940; sialic acid binding Ig-like lectin 10; sialic acid-binding Ig-like lectin 10; Siglec10; Siglec-10; siglec-like gene 2; Siglec-like protein 2; SLG2; SLG2sialic acid binding Ig-like lectin 10 Ig-like lectin 7