Human Siglec-5/Siglec-14 PE-conjugated Antibody

Name: Human Siglec-5/Siglec-14 PE-conjugated Antibody
Brand: R&D Systems
SKU: FAB10721P

Catalog #: FAB10721P Datasheet / COA / SDS

Discontinued Product

FAB10721P has been discontinued.

View all Siglec-5/Siglec-14 products.

Detection of Siglec‑5/Siglec‑14 in Human Blood Monocytes by Flow Cytometry.

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All Siglec-5/Siglec-14 Antibodies

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Human Siglec-5/Siglec-14 PE-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human Siglec‑5/Siglec‑14 in ELISAs. In sandwich immunoassays, 100% cross-reactivity with recombinant human (rh) Siglec-14 is observed and no cross-reactivity with rhSiglec-3, rhSiglec-7, or rhSiglec-9 is observed.

Source

Monoclonal Mouse IgG₁ Clone # 194128

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Siglec‑5/Siglec‑14
Lys18-Thr434
Accession # O15389

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of Siglec‑5/Siglec‑14 in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes were stained with Mouse Anti-Human Siglec-5/Siglec-14 PE-conjugated Monoclonal Antibody (Catalog # FAB10721P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: Siglec-5/Siglec-14

Siglecs (sialic acid binding Ig-like lectins) are a subgroup of the immunoglobulin superfamily that interact with sialic acids in glycoproteins and glycolipids. Siglec-5 binds to alpha-2, 3- and alpha-2, 6-linked sialic acid equally. It occurs as a disulfide-linked dimer of approximately 140 kDa with the highest expression levels in hematopoietic tissues. Siglec-5 has an inhibitory motif within its cytoplasmic domain. Siglec-14 is an activating receptor that shares 99.5% aa sequence identity with Siglec-5 through the first two extracellular Ig domains and displays a similar glycan binding preference.

Long Name

Sialic Acid Binding Ig-like Lectin 5

Entrez Gene IDs

8778 (Human)

Alternate Names

CD170;CD33L2;OBBP2;OB-BP2;Sialic acid-binding Ig-like lectin 5;SIGLEC5;SIGLEC-5; Siglec-5/Siglec-14