Human Soggy-1/DkkL1 Biotinylated Antibody

Catalog #: BAF1549 Datasheet / COA / SDS

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BAF1549

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Human Soggy-1/DkkL1 Biotinylated Antibody Summary

Species Reactivity

Human

Specificity

Detects human Soggy-1/DkkL1 in Western blots. In Western blots, approximately 5% cross‑reactivity with recombinant mouse Soggy-1 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Soggy-1/DkkL1
Ala31-Leu242
Accession # Q9UK85

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human Soggy‑1 (Catalog # 1549-S1)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Soggy-1/DkkL1

Soggy-1, also known as DkkL1 (Dickkopf-like 1), is a secreted protein that is related to the Dickkopf family of Wnt antagonists. Dkk-1, -2, -3, and -4 each have two cysteine-rich domains separated by a linker. Soggy-1 does not contain cysteine-rich domains but bears some sequence homology with the N-terminal regions of Dkk proteins (1, 2). The human Soggy-1 cDNA encodes a 242 amino acid (aa) precursor that includes a 30 aa signal sequence (2). Human Soggy-1 shares approximately 65% aa sequence identity with mouse and rat Soggy-1 and 15 - 20% aa sequence identity with human Dkk-1, -2, -3, and -4. Mouse Soggy-1 is expressed at various sites in the embryo but in the adult is primarily found in the testes (3 - 5). Soggy-1 transcription is regulated by the spermatocyte specific factor, RFX2 (6). The regulatory elements for Soggy-1 lie very close to those of TEAD-2, a transcription factor that is expressed very early in development (4). Soggy-1 and TEAD-2 are co-expressed in preimplantation embryos and embryonic stem cells, but differentiated cells express only one or the other (4, 5). During development, Soggy-1 is first detectable at the onset of sexual differentiation (4). Soggy-1 is localized to the acrosome in developing spermatocytes and mature spermatozoa (3, 4). Soggy-1, as expressed in developing mouse spermatocytes, is a 34 kDa N-glycosylated protein. This glycosylation is not present on Soggy-1 in mature spermatozoa, although the apparent molecular weight suggests the presence of some post-translational modification (3). Two shorter forms of Soggy-1 have been described that result from the use of internal methionine residues for initiation (4).

References

Kawano, Y. and R. Kypta (2003) J. Cell Sci. 116:2627.
Krupnik, V.E. et al. (1999) Gene 238:301.
Kohn, M.J. et al. (2005) Mol. Reprod. Dev. 71:516.
Kaneko, K.J. and M.L. DePamphilis (2000) Nucleic Acids Res. 28:3982.
Kaneko, K.J. et al. (2004) Mol. Cell. Biol. 24:1968.
Horvath, G.C. et al. (2004) Biol. Reprod. 71:1551.

Long Name

Dickkopf-like Protein 1

Entrez Gene IDs

27120 (Human); 50722 (Mouse)

Alternate Names

Cancer/testis antigen 34; CT34dickkopf-like 1 (soggy); dickkopf-like 1; DkkL1; Protein soggy-1; SGY; SGY1; SGY-1dickkopf-like protein 1; SGY1soggy; Soggy1; Soggy-1