Human SOX11 Antibody

Catalog #: AF3976 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF3976
AF3976-SP

Detection of Human SOX11 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human SOX11 Antibody Summary

Species Reactivity

Human

Specificity

Detects human SOX11 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) SOX1, rhSOX2, rhSOX3, rhSOX5, rhSOX6, rhSOX7, rhSOX9, rhSOX10, rhSOX12, rhSOX14, rhSOX15, rhSOX17, and rhSOX21 is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human SOX11
Val2-Pro173
Accession # P35716

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Immunohistochemistry

3-15 µg/mL

See below

Immunocytochemistry

5-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human SOX11 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line. Gels were loaded with 25 µg of cytoplasmic (Cyto) and 25 µg of nuclear (Nuc) extracts. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human SOX11 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3976) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for SOX11 at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry

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SOX11 in HeLa Human Cell Line. SOX11 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Sheep Anti-Human SOX11 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3976) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red, upper panel; Catalog # NL010) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunohistochemistry

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SOX11 in Human Brain. SOX11 was detected in immersion fixed paraffin-embedded sections of human brain (hippocampus) using Sheep Anti-Human SOX11 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3976) at 3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS020) and counterstained with hematoxylin (blue). Specific staining was localized to neurons. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SOX11

Human SOX11 (SRY box-related gene 11) is a 60-72 kDa (46.6 kDa predicted), group C member of the SOX family of transcription factors. It is 441 amino acids (aa) in length, and contains one HMG box (aa 49-117), two poly-Gly regions (aa 144-214), one poly-Asp domain (aa 223-233) and one poly-Ser region. The HMG box determines DNA and transcriptional coactivator binding, the poly-Ser region lies in a potent transactivating region, and the poly-Asp domain autoinhibits/regulates activities of the other two regions. SOX11 appears early in development in multiple tissues, and impacts neuron, oligodendrocyte and cartilage formation. Human SOX11 is 94% aa identical to mouse SOX11 over aa 1-173. Mouse SOX11, however, is absent a 12 aa poly-Gly sequence that is present in human SOX11 between aa 145-156. Additional band correspondent to the lower molecular weight may represent SOX11 without posttranslational modifications or human SOX4, over aa 38-123 human SOX11 shares 100% aa identity with human SOX4. (1)

References