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Human SOX17 Antibody

Catalog #: MAB19241
8 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB19241
MAB19241-SP
SOX17 in BG01V Human Stem Cells.
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Product Details
Citations (8)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human SOX17 Antibody Summary

Species Reactivity
Human
Specificity
Detects human SOX17 in direct ELISAs.
Source
Monoclonal Mouse IgG1 Clone # 614013
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human SOX17
Asp177-Val414
Accession # Q9H6I2
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Immunocytochemistry
8-25 µg/mL
See below
Intracellular Staining by Flow Cytometry
0.25 µg/106 cells
Endoderm-differentiated BG01V human embryonic stem cells; iPSCs

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry SOX17 antibody in BG01V Human Stem Cells by Immunocytochemistry (ICC). View Larger

SOX17 in BG01V Human Stem Cells. SOX17 was detected in immersion fixed endoderm-differentiated BG01V human embryonic stem cells using Mouse Anti-Human SOX17 Monoclonal Antibody (Catalog # MAB19241) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow, upper panel; NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Intracellular Staining by Flow Cytometry View Larger

Detection of SOX17 in Endoderm Differentiated BG01V hESCs by Flow Cytometry Endoderm differentiated BG01V hESCs were stained with Mouse Anti-Human SOX17 Monoclonal Antibody (Catalog # MAB19241, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules. Endoderm differentiation was performed using SC019B following protocol on the datasheet.

Flow (Intracellular) View Larger

Detection of SOX17 in iPSCs by Flow Cytometry iPSCs were stained with Mouse Anti-Human SOX17 Monoclonal Antibody (Catalog # MAB19241, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SOX17

SOX17 is a member of the SOX family of transcription factors that bind DNA via a high mobility group (HMG) domain. SOX17 is suggested to play an important role in endoderm development (1, 2).

References
  1. Kanai-Azuma, M. et al. (2002) Development 129:2367.
  2. Katoh, M. et al. (2002) Int. J. Mol. Med. 9:153.
Long Name
Transcription Factor SOX17
Entrez Gene IDs
64321 (Human)
Alternate Names
FLJ22252; SOX17; SRY (sex determining region Y)-box 17; SRY-related HMG-box transcription factor SOX17; transcription factor SOX-17; VUR3

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Citations for Human SOX17 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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  1. Differentiation of induced pluripotent stem cells into definitive endoderm on Activin A-functionalized gradient surfaces
    Authors: L Andreasson, H Evenbratt, R Mobini, S Simonsson
    J Biotechnol, 2020-11-02;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  2. Direct On-Chip Differentiation of Intestinal Tubules from Induced Pluripotent Stem Cells
    Authors: E Naumovska, G Aalderink, C Wong Valen, K Kosim, A Nicolas, S Brown, P Vulto, KS Erdmann, D Kurek
    Int J Mol Sci, 2020-07-14;21(14):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  3. Establishment of a human induced pluripotent stem cell (iPSC) line (HIHDNEi002-A) from a patient with developmental and epileptic encephalopathy carrying a KCNA2 (p.Arg297Gln) mutation
    Authors: N Schwarz, B Uysal, F Rosa, H Löffler, UA Mau-Holzma, S Liebau, H Lerche
    Stem Cell Res, 2019-04-19;37(0):101445.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  4. Generation of an induced pluripotent stem cell (iPSC) line from a patient with developmental and epileptic encephalopathy carrying a KCNA2 (p.Leu328Val) mutation
    Authors: N Schwarz, B Uysal, F Rosa, H Löffler, UA Mau-Holzma, S Liebau, H Lerche
    Stem Cell Res, 2018-08-31;33(0):6-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  5. Human-Induced Pluripotent Stem Cells Manufactured Using a Current Good Manufacturing Practice-Compliant Process Differentiate Into Clinically Relevant Cells From Three Germ Layers
    Authors: M Shafa, F Yang, T Fellner, MS Rao, BA Baghbadera
    Front Med (Lausanne), 2018-03-15;5(0):69.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  6. Bulk cell density and Wnt/TGFbeta signalling regulate mesendodermal patterning of human pluripotent stem cells
    Nat Commun, 2016-12-09;7(0):13602.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  7. A programmable synthetic lineage-control network that differentiates human IPSCs into glucose-sensitive insulin-secreting beta-like cells
    Authors: P Saxena, BC Heng, P Bai, M Folcher, H Zulewski, M Fussenegge
    Nat Commun, 2016-04-11;7(0):11247.
    Species: Human
    Sample Types: Whole Cells
    Applications: IHC
  8. Long-Term Stability and Differentiation Potential of Cryopreserved cGMP-Compliant Human Induced Pluripotent Stem Cells
    Authors: Mehdi Shafa, Tylor Walsh, Krishna Morgan Panchalingam, Thomas Richardson, Laura Menendez, Xinghui Tian et al.
    International Journal of Molecular Sciences

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