Human SPINK1 Antibody

Catalog #: AF7496 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF7496
AF7496-SP

Detection of Human SPINK1 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human SPINK1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human SPINK1 in direct ELISAs and Western blots.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human SPINK1
Asp24-Cys79
Accession # P00995

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.5 µg/mL

See below

Simple Western

5 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human SPINK1 by Western Blot. Western blot shows lysates of human pancreas tissue. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human SPINK1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7496) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for SPINK1 at approximately 6 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western

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Detection of Human SPINK1 by Simple Western^TM. Simple Western lane view shows lysates of human pancreas tissue, loaded at 0.2 mg/mL. A specific band was detected for SPINK1 at approximately 5 kDa (as indicated) using 5 µg/mL of Sheep Anti-Human SPINK1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7496) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Simple Western

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Detection of Human SPINK1 by Simple Western^TM. Simple Western lane view shows lysates of undifferentiated Ileum stem cells and day 12 differentiation Ileum stem cells, loaded at 0.2 mg/mL. A specific band was detected for SPINK1 at approximately 6 kDa (as indicated) using 5 µg/mL of Sheep Anti-Human SPINK1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7496) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 2-40 kDa separation system.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SPINK1

SPINK1 (Serine Protease Inhibitor Kazal-type 1; also TATI and PST1) is a 6-7 kDa secreted polypeptide initially identified as a tumor-derived trypsin inhibitor. It is widely expressed, and found in cells diverse as pancreatic acinar cells, columnar cells of the stomach, renal collecting duct epithelium, and ureteric transitional plus breast epithelium. SPINK1 is known to be secreted with pancreatic zymogens, and apparently inactivates prematurely-activated trypsin, thus protecting the pancreas from trypsin-mediated enzyme activation. It also is reported to regulate cell migration and proliferation, the latter effect attributed to its structural resemblance to EGF, and its ability to bind to activate the EGFR. Mature human SPINK1 is 56 amino acids (aa) in length (aa 24-79). It contains one Kazal-like domain (aa 26-79) that possesses a potential proteolytic cleavage site between Lys41-Ile42. An 11-12 kDa form in SDS-PAGE has been reported for SPINK1, possibly reflecting dimerization. Mature human SPINK1 shares 66% aa sequence identity with mouse Spink3, the mouse equivalent to human SPINK1.

Long Name

Serine Peptidase Inhibitor, Kazal Type 1

Entrez Gene IDs

6690 (Human); 20730 (Mouse); 266602 (Rat)

Alternate Names

pancreatic secretory trypsin inhibitor; PCTT; PCTTSpink3; PSTI; PSTISerine protease inhibitor Kazal-type 1; serine peptidase inhibitor, Kazal type 1; serine protease inhibitor, Kazal type 1; SPINK1; Spink3; TATI; TATITumor-associated trypsin inhibitor