Human STEAP1 Antibody

Name: Human STEAP1 Antibody
Brand: R&D Systems
SKU: AF5587
Rating: 4 (1 reviews)

Catalog #: AF5587 1 Review Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF5587
AF5587-SP

Detection of Human STEAP1 by Western Blot.

2 Images

All STEAP1 Antibodies

Product Details

FAQs

Supplemental Products

Reviews (1)

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human STEAP1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human STEAP1 in direct ELISAs and Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human STEAP1
Met1-His72
Accession # Q9UHE8

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Simple Western

10 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

View Larger

Detection of Human STEAP1 by Western Blot. Western blot shows lysates of LNCaP human prostate cancer cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human STEAP1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5587) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for STEAP1 at approximately 37 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Simple Western

View Larger

Detection of Human STEAP1 by Simple Western^TM. Simple Western lane view shows lysates of LNCaP human prostate cancer cell line, loaded at 0.2 mg/mL. A specific band was detected for STEAP1 at approximately 40 kDa (as indicated) using 10 µg/mL of Goat Anti-Human STEAP1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5587) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: STEAP1

STEAP1 (six-transmembrane epithelial antigen of the prostate-1) is a 40 kDa protein (predicted) of the STEAP family of metalloreductases. It is expressed mainly at cell‑cell junctions between prostate secretory epithelium, and up‑regulated in prostate and some bladder, colon and ovarian cancers and Ewing’s sarcomas. Human STEAP1 is 339 amino acids (aa) in length. It contains a ferric oxidoreductase domain (aa 119‑265) that includes transmembrane sequences (# 2, 3, 4, and part of 5, out of 6 sequences). Two splice forms diverge at aa 255, terminating after aa 58 or 259. Over aa 1‑71, human and mouse STEAP1 share 68% aa identity.

Long Name

Six Transmembrane Epithelial Antigen of the Prostate 1

Entrez Gene IDs

26872 (Human); 70358 (Mouse); 297738 (Rat)

Alternate Names

EC 1.16.1.-; PRSS24; PRSS24MGC19484; six transmembrane epithelial antigen of the prostate 1; six transmembrane epithelial antigen of the prostate; Six-transmembrane epithelial antigen of prostate 1; STEAP1; STEAPmetalloreductase STEAP1