Human Survivin PE-conjugated Antibody

Catalog #: IC6472P
4 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
IC6472P

Detection of Survivin in Human Blood Lymphocytes by Flow Cytometry.

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All Survivin Antibodies

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Citations (4)

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Human Survivin PE-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human Survivin in direct ELISAs.

Source

Monoclonal Mouse IgG₁ Clone # 91630

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human Survivin
Met1-Asp142
Accession # O15392

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration

Sample

Intracellular Staining by Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry

Detection of Survivin antibody in Human Blood Lymphocytes antibody by Flow Cytometry.

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Detection of Survivin in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Mouse Anti-Human Survivin PE-conjugated Monoclonal Antibody (Catalog # IC6472P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: Survivin

Survivin is a member of the Inhibitor of Apoptosis (IAP) family and can inhibit apoptosis induced by a variety of factors. It is expressed in most human cancers but not in normal adult tissues. Survivin is expressed in a cell cycle-dependent manner and associates with the mitotic apparatus.

Entrez Gene IDs

332 (Human)

Alternate Names

API4; apoptosis inhibitor 4; Apoptosis inhibitor survivin; baculoviral IAP repeat-containing 5; Baculoviral IAP Repeat-Containing Protein 5; BIRC5; EPR-1; IAP4; survivin variant 3 alpha; Survivin

Product Datasheets

Product Datasheet

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Citations for Human Survivin PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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Quantitative Analysis of Survivin Protein Expression and Its Therapeutic Depletion by an Antisense Oligonucleotide in Human Lung Tumors
Authors: Anna L Olsen, Joanne M Davies, Louise Medley, David Breen, Denis C Talbot, Peter J McHugh
Molecular Therapy - Nucleic Acids
Survivin-specific T cell receptor targets tumor but not T cells.
Authors: Arber C, Feng X, Abhyankar H, Romero E, Wu M, Heslop H, Barth P, Dotti G, Savoldo B
J Clin Invest, 2014-11-21;125(1):157-68.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
Activation of Fms-like tyrosine kinase 3 signaling enhances survivin expression in a mouse model of rheumatoid arthritis.
Authors: Andersson S, Svensson M, Erlandsson M, Dehlin M, Andersson K, Bokarewa M
PLoS ONE, 2012-10-17;7(10):e47668.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry
Extracellular survivin up-regulates adhesion molecules on the surface of leukocytes changing their reactivity pattern.
Authors: Mera S, Magnusson M, Tarkowski A, Bokarewa M
J. Leukoc. Biol., 2007-10-15;83(1):149-55.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry