Human SUSD2 Antibody

Catalog #: MAB90561 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB90561
MAB90561-SP

Detection of SUSD2 in HEK293 Human Cell Line Transfected with Human SUSD2 and eGFP by Flow Cytometry.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human SUSD2 Antibody Summary

Species Reactivity

Human

Specificity

Detects human SUSD2 in direct ELISAs. Stains human SUSD2 transfectants but not irrelevant transfectants in flow cytometry.

Source

Monoclonal Mouse IgG_2A Clone # 944827

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Human embryonic kidney cell line HEK293-derived human SUSD2
Met1-Ala785
Accession # Q9UGT4

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/mL

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of SUSD2 in HEK293 Human Cell Line Transfected with Human SUSD2 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with either (A) human SUSD2 or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human SUSD2 Monoclonal Antibody (Catalog # MAB90561) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB003). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SUSD2

Sushi domain containing 2, or SUSD2, is a type I transmembrane protein of 822 amino acids containing functional domains inherent to adhesion molecules. SUSD2 has been described as a novel marker of human endometrial mesenchymal stem-like cells and it has been used for their prospective isolation. As a transmembrane receptor, SUSD2 has been proposed to interact with Galectin-1 and to be the receptor for C10ORF99, a novel potential cytokine suggested to inhibit colon cancer cell growth through inducing G1 arrest. There is evidence that SUSD2 may play a role in breast tumorigenesis.

Long Name

Sushi Domain Containing 2

Entrez Gene IDs

56241 (Human); 71733 (Mouse); 294335 (Rat)

Alternate Names

BK65A6.2; FLJ22778; SUSD2; Sushi domain (SCR repeat) containing; sushi domain containing 2; sushi domain-containing protein 2