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Human TBX18 Antibody

Catalog #: MAB63371
4 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB63371
MAB63371-SP
TBX18 in Saos‑2 Human Cell Line.
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Product Details
Citations (4)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human TBX18 Antibody Summary

Species Reactivity
Human
Specificity
Detects human TBX18 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) TBX2, 3, 5, 6, 20, or rhT-bet is observed.
Source
Monoclonal Mouse IgG2B Clone # 635305
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human TBX18
Ala384-Ser504
Accession # O95935
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry TBX18 antibody in Saos-2 Human Cell Line by Immunocytochemistry (ICC). View Larger

TBX18 in Saos‑2 Human Cell Line. TBX18 was detected in immersion fixed Saos-2 human osteosarcoma cell line using Mouse Anti-Human TBX18 Monoclonal Antibody (Catalog # MAB63371) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunocytochemistry View Larger

TBX18 in A549 Human Cell Line. TBX18 was detected in immersion fixed A549 human lung carcinoma cell line (positive staining) and MCF‑7 human breast cancer cell line using Mouse Anti-Human TBX18 Monoclonal Antibody (Catalog # MAB63371) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to cell nuclei. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TBX18

TBX18 is a 607 amino acid, 65 kDa (predicted) protein that is a member of the T-box family of developmentally expressed transcription factors. It is developmentally expressed in the anterior portion of the head, somites and limb buds. It is expressed on pluripotent progenitors in the developing epicardium where it is required for formation of the cardiac AV node, and can also contribute to fibroblast and smooth muscle formation. Within the region used as an immunogen, human TBX18 shares 93% amino acid identity with mouse and rat TBX18.

Long Name
T-box Transcription Factor 18
Entrez Gene IDs
9096 (Human); 76365 (Mouse); 315870 (Rat)
Alternate Names
T-box 18; T-box protein 18; T-box transcription factor TBX18; TBX18

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Citations for Human TBX18 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Human Pluripotent Stem Cell-Derived Multipotent Vascular Progenitors of the Mesothelium Lineage Have Utility in Tissue Engineering and Repair
    Authors: T Colunga, M Hayworth, S Kre beta, DM Reynolds, L Chen, KL Nazor, J Baur, AM Singh, JF Loring, M Metzger, S Dalton
    Cell Rep, 2019-03-05;26(10):2566-2579.e10.
  2. Co-emergence of cardiac and gut tissues promotes cardiomyocyte maturation within human iPSC-derived organoids
    Authors: AC Silva, OB Matthys, DA Joy, MA Kauss, V Natarajan, MH Lai, D Turaga, AP Blair, M Alexanian, BG Bruneau, TC McDevitt
    Cell Stem Cell, 2021-12-02;28(12):2137-2152.e6.
    Species: Human
    Sample Types: Organoid
    Applications: IHC
  3. Multipotent Vascular Progenitor Cells of the Mesothelium Lineage Generated from Human Pluripotent Stem Cells
    Authors: Luoman Chen, Stephen Dalton
    STAR Protocols
  4. Differential expression of embryonic epicardial progenitor markers and localization of cardiac fibrosis in adult ischemic injury and hypertensive heart disease
    Authors: Caitlin M. Braitsch, Onur Kanisicak, Jop H. van Berlo, Jeffery D. Molkentin, Katherine E. Yutzey
    Journal of Molecular and Cellular Cardiology

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