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Human TGF-beta  RI/ALK-5 Biotinylated Antibody

Catalog #: BAF3025
1 Citation 1 Review Datasheet / COA / SDS
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BAF3025
Product Details
Citations (1)
FAQs
Supplemental Products
Reviews (1)
Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human TGF-beta  RI/ALK-5 Biotinylated Antibody Summary

Species Reactivity
Human
Specificity
Detects human TGF-beta RI/ALK-5 in Western blots.  In Western blots, approximately 50% cross-reactivity with recombinant mouse TGF-beta RI and less than 5% cross-reactivity with recombinant human (rh) TGF-beta RII and rhTGF-beta RIII is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human TGF-beta RI/ALK-5
Leu34-Glu125
Accession # P36897
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human TGF-beta RI/ALK-5

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TGF-beta RI/ALK-5

Human TGF-beta RI (also ALK5) is a 55 kDa member of the Ser/Thr kinase family of transmembrane proteins. It is a type I membrane glycoprotein that is 479 amino acids (aa) in length. The short 101 aa extracellular domain (ECD) contains multiple cysteines and one potential N-linked glycosylation site. It is ubiquitously expressed, and forms a functional heterotetrameric TGF-beta receptor with TGF-beta RII. TGF-beta binding to TGF-beta RII phosphorylates TGF-beta RI, which then activates SMAD transcriptional proteins. The human TGF-beta RI ECD is 91%, 90%, 91%, and 93% aa identical to the TGF-beta RI ECD in pig, mouse, cow and rat, respectively.

Long Name
Transforming Growth Factor beta Receptor I
Entrez Gene IDs
7046 (Human); 21812 (Mouse); 29591 (Rat)
Alternate Names
AAT5; activin A receptor type II-like kinase, 53kD; activin A receptor type II-like kinase, 53kDa; Activin receptor-like kinase 5; ACVRLK4; ALK-5; ALK-5ALK5; EC 2.7.11; EC 2.7.11.30; LDS1A; LDS2A; Serine/threonine-protein kinase receptor R4; SKR4; tbetaR-I; TGFB1R1; TGF-beta receptor type I; TGF-beta receptor type-1; TGF-beta RI; TGF-beta type I receptor; TGFbetaRI; TGFBR1; TGF-bRI; TGFR-1; transforming growth factor beta receptor I; transforming growth factor, beta receptor 1; transforming growth factor, beta receptor I (activin A receptor type II-likekinase, 53kD); Transforming growth factor-beta receptor type I

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Citation for Human TGF-beta  RI/ALK-5 Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. BMP-7 induces apoptosis in human germinal center B cells and is influenced by TGF-? receptor type I ALK5
    Authors: LK Bollum, K Huse, MP Oksvold, B Bai, VI Hilden, L Forfang, SO Yoon, S Wälchli, EB Smeland, JH Myklebust
    PLoS ONE, 2017-05-10;12(5):e0177188.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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Human TGF-beta  RI/ALK-5 Biotinylated Antibody
By Anonymous on 09/17/2021
Application: ELISA Sample Tested: Recombinant protein Species: Human

Immobilized TBRII onto ELISA plate. Then incubated plate with TGFb1 + TBRI solution. Detect binding of TBRI to TGFb1+TBRII complex by using Anti-TBRI-biotin. No binding of TBRI only to plate.

ELISA Human TGF-beta  RI/ALK-5 Biotinylated Antibody BAF3025