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Human TIMP-1 Antibody

Catalog #: AF970
23 Citations 2 Reviews Datasheet / COA / SDS
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AF970
AF970-SP
Detection of Human TIMP‑1 by Western Blot.
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Product Details
Citations (23)
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Reviews (2)
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Human TIMP-1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human TIMP-1 in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant mouse TIMP‑1 and recombinant rat TIMP-1 is observed and less than 1% cross-reactivity with recombinant human (rh) TIMP-2, rhTIMP-3, and rhTIMP‑4 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human TIMP-1
Cys24-Ala207
Accession # Q6FGX5
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1-5 µg/mL
See below
Simple Western
50 µg/mL
See below
Immunohistochemistry
1-15 µg/mL
See below
Knockout Validated
TIMP‑1 is specifically detected in SK‑OV‑3 human ovarian adenocarcinoma parental cell line but is not detectable in TIMP‑1 knockout SK‑OV‑3 cell line.
 
Neutralization
Measured by its ability to neutralize Recombinant Human TIMP-1 (0.1 µg/mL, Catalog # 970-TM) inhibition of Recombinant Human MMP‑2 (0.2 µg/mL, Catalog # 902-MP) cleavage of the fluorogenic peptide substrate Mca‑PLGL-Dpa-AR-NH2 (5 µM, Catalog # ES001). The Neutralization Dose (ND50) is typically 1 µg/mL.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human TIMP-1 antibody by Western Blot. View Larger

Detection of Human TIMP‑1 by Western Blot. Western blot shows lysates of human lung tissue and human prostate tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human TIMP-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF970) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for TIMP-1 at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Western Blot Detection of Human TIMP-1 antibody by Western Blot. View Larger

Detection of Human TIMP‑1 by Western Blot. Western blot shows lysates of PC-3 human prostate cancer cell line, HT-29 human colon adenocarcinoma cell line, and SK-OV-3 human ovarian adenocarcinoma cell line. PVDF membrane was probed with 5 µg/mL of Goat Anti-Human TIMP-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF970) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for TIMP-1 at approximately 26 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry TIMP-1 antibody in Human Colon Cancer Tissue by Immunohistochemistry (IHC-P). View Larger

TIMP‑1 in Human Colon Cancer Tissue. TIMP-1 was detected in immersion fixed paraffin-embedded sections of human colon cancer tissue using Goat Anti-Human TIMP-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF970) at 1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and extracellular space. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Simple Western Detection of Human TIMP-1 antibody by Simple Western<sup>TM</sup>. View Larger

Detection of Human TIMP‑1 by Simple WesternTM. Simple Western lane view shows lysates of PC-3 human prostate cancer cell line, HT-29 human colon adenocarcinoma cell line, and human lung tissue, loaded at 0.2 mg/mL. A specific band was detected for TIMP-1 at approximately 42-45 kDa (as indicated) using 50 µg/mL of Goat Anti-Human TIMP-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF970) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Knockout Validated Western Blot Shows Human TIMP-1 Antibody Specificity by Using Knockout Cell Line. View Larger

Western Blot Shows Human TIMP‑1 Specificity by Using Knockout Cell Line. Western blot shows lysates of SK-OV-3 human ovarian adenocarcinoma cell line and TIMP-1 knockout SK-OV-3 cell line (KO). PVDF membrane was probed with 2 µg/mL of Goat Anti-Human TIMP-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF970) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for TIMP-1 at approximately 25 kDa (as indicated) in the parental SK-OV-3 cell line, but is not detectable in knockout SK-OV-3 cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Neutralization Neutralization of TIMP‑1 Activity by Human TIMP‑1 Antibody. View Larger

Neutralization of TIMP‑1 Activity by Human TIMP‑1 Antibody. Recombinant Human MMP-2 (0.2 µg/mL, Catalog # 902-MP) activity is measured in the presence of Recombinant Human TIMP-1 (0.1 µg/mL, Catalog # 970-TM) that has been preincubated with increasing concentrations of Goat Anti-Human TIMP-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF970). The ND50 is typically 1 µg/mL.

Immunohistochemistry-Paraffin Detection of Human TIMP-1 by Immunohistochemistry-Paraffin View Larger

Detection of Human TIMP-1 by Immunohistochemistry-Paraffin IHC staining result investigating TR4 level and macrophage infiltration in tumor tissues of PCa patients, as well as TIMP-1/MMP2/MMP9 signaling. IHC staining was performed using TR4 antibody (1:300),CD68 antibody (1:200),TIMP-1 antibody (1:200),MMP2 antibody (1:200),MMP9 antibody (1:200). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/25623427), licensed under a CC-BY license. Not internally tested by R&D Systems.

Proximity Ligation Assay Detection of Human TIMP-1 by Proximity Ligation Assay View Larger

Detection of Human TIMP-1 by Proximity Ligation Assay Univariate survival analysis of disease free survival (DFS) in plasma for all 465 patients. A) MMP-9:TIMP-1 measured by ELISA. B) MMP-9:TIMP-1 measured by PLA. Patients are divided into four groups of equal size (Q1-Q4) according to increasing plasma MMP-9:TIMP-1 levels; Q1 being the group with the lowest level. Image collected and cropped by CiteAb from the following open publication (https://bmccancer.biomedcentral.com/articles/10.1186/1471-2407-13-598), licensed under a CC-BY license. Not internally tested by R&D Systems.

Proximity Ligation Assay Detection of Human TIMP-1 by Proximity Ligation Assay View Larger

Detection of Human TIMP-1 by Proximity Ligation Assay Univariate survival analysis of disease free survival (DFS) in plasma for all 465 patients. A) MMP-9:TIMP-1 measured by ELISA. B) MMP-9:TIMP-1 measured by PLA. Patients are divided into four groups of equal size (Q1-Q4) according to increasing plasma MMP-9:TIMP-1 levels; Q1 being the group with the lowest level. Image collected and cropped by CiteAb from the following open publication (https://bmccancer.biomedcentral.com/articles/10.1186/1471-2407-13-598), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TIMP-1

Tissue inhibitors of metalloproteinases or TIMPs are a family of proteins that regulate the activation and proteolytic activity of the zinc enzymes known as matrix metalloproteinases (MMPs). There are four members of the family, TIMP-1, TIMP-2, TIMP-3 and TIMP-4. TIMP-1 is a glycoprotein with a molecular mass of 28 kDa produced by a wide range of cell types. TIMP-1 inhibits active MMP-mediated proteolysis by forming an N-terminal, non-covalent binary complex with the MMP active site. TIMP-1 also associates C-terminally with Pro-MMP-9 in a complex which may play a role in regulating activation. Independent of MMPs, TIMP-1 has been shown to have a role in tissue homeostasis.

Long Name
Tissue Inhibitors of Metalloproteinases 1
Entrez Gene IDs
7076 (Human); 21857 (Mouse); 116510 (Rat)
Alternate Names
CLGI; Collagenase inhibitor; collagenase inhibitor); EPATIMP-1; EPO; erythroid potentiating activity; Erythroid-potentiating activity; Fibroblast collagenase inhibitor; FLJ90373; HCI; metalloproteinase inhibitor 1; TIMP metallopeptidase inhibitor 1; TIMP1; TIMP-1; TIMPtissue inhibitor of metalloproteinase 1 (erythroid potentiating activity; Tissue inhibitor of metalloproteinases 1

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Citations for Human TIMP-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

23 Citations: Showing 1 - 10
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  1. CD49f Is a Novel Marker of Functional and Reactive Human iPSC-Derived Astrocytes
    Authors: Barbar L, Jain T, Zimmer M et al.
    Neuron
  2. The periosteum provides a stromal defence against cancer invasion into the bone
    Authors: Nakamura, K;Tsukasaki, M;Tsunematsu, T;Yan, M;Ando, Y;Huynh, NC;Hashimoto, K;Gou, Q;Muro, R;Itabashi, A;Iguchi, T;Okamoto, K;Nakamura, T;Nakano, K;Okamura, T;Ueno, T;Ito, K;Ishimaru, N;Hoshi, K;Takayanagi, H;
    Nature
    Species: Mouse, Transgenic Mouse
    Sample Types: Whole Tissue
    Applications: Immunohistochemistry
  3. Myeloma Microenvironmental TIMP1 Induces the Invasive Phenotype in Fibroblasts to Modulate Disease Progression
    Authors: R Ishihara, T Oda, Y Murakami, I Matsumura, S Watanabe, Y Asao, Y Masuda, N Gotoh, T Kasamatsu, H Takei, N Kobayashi, N Sasaki, T Saitoh, H Murakami, H Handa
    International Journal of Molecular Sciences, 2023-01-22;24(3):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  4. Inhibition of Monoacylglycerol Lipase Decreases Angiogenic Features of Endothelial Cells via Release of Tissue Inhibitor of Metalloproteinase-1 from Lung Cancer Cells
    Authors: Wittig, F;Henkel, L;Prüser, J;Merkord, J;Ramer, R;Hinz, B;
    Cells
    Species: Human
    Sample Types: Whole Tissue
    Applications: Immunohistochemistry
  5. Improved contractile potential in detrusor microtissues from pediatric patients with end stage lower urinary tract dysfunction
    Authors: Gerwinn T, Salemi S, Schori LJ et al.
    Frontiers in Cell and Developmental Biology
  6. Single cell transcriptomic landscape of diabetic foot ulcers
    Authors: G Theocharid, BE Thomas, D Sarkar, HL Mumme, WJR Pilcher, B Dwivedi, T Sandoval-S, RF Sîrbulescu, A Kafanas, I Mezghani, P Wang, A Lobao, IS Vlachos, B Dash, HC Hsia, V Horsley, SS Bhasin, A Veves, M Bhasin
    Nature Communications, 2022-01-10;13(1):181.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  7. Inhibition of epithelial-mesenchymal transition in retinal pigment epithelial cells by a retinoic acid receptor-&alpha agonist
    Authors: Y Kobayashi, K Tokuda, C Yamashiro, F Higashijim, T Yoshimoto, M Ota, T Ogata, A Ashimori, M Hatano, M Kobayashi, SH Uchi, M Wakuta, K Kimura
    Scientific Reports, 2021-06-04;11(1):11842.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  8. Heroin Seeking and Extinction From Seeking Activate Matrix Metalloproteinases at Synapses on Distinct Subpopulations of Accumbens Cells
    Authors: Vivian C. Chioma, Anna Kruyer, Ana-Clara Bobadilla, Ariana Angelis, Zachary Ellison, Ritchy Hodebourg et al.
    Biological Psychiatry
  9. IL35 predicts prognosis in gastric cancer and is associated with angiogenesis by altering TIMP1, PAI1, and IGFBP1
    Authors: X Li, N Niu, J Sun, Y Mou, X He, L Mei
    FEBS Open Bio, 2020-11-09;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  10. The Metalloproteinase ADAMTS5 Is Expressed by Interstitial Inflammatory Cells in IgA Nephropathy and Is Proteolytically Active on the Kidney Matrix
    Authors: S Taylor, M Whitfield, J Barratt, A Didangelos
    J. Immunol., 2020-09-11;0(0):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  11. Circulating Tumor Cells Characterization Revealed TIMP1 as a Potential Therapeutic Target in Ovarian Cancer
    Authors: M Abreu, P Cabezas-Sa, L Alonso-Alc, A Ferreirós, P Mondelo-Ma, RM Lago-Lestó, A Abalo, E Díaz, S Palacios-Z, A Rojo-Sebas, R López-Lópe, L Sánchez, G Moreno-Bue, L Muinelo-Ro
    Cells, 2020-05-14;9(5):.
    Species: Human
    Sample Types: Whole Cell
    Applications: Western Blot
  12. Looking for a Better Characterization of Triple-Negative Breast Cancer by Means of Circulating Tumor Cells
    Authors: M Abreu, P Cabezas-Sa, T Pereira-Ve, C Falo, A Abalo, I Morilla, T Curiel, J Cueva, C Rodríguez, V Varela-Pos, R Lago-Lestó, P Mondelo, P Palacios, G Moreno-Bue, A Cano, T García-Cab, MÁ Pujana, L Sánchez-Pi, C Costa, R López, L Muinelo-Ro
    J Clin Med, 2020-01-27;9(2):.
    Species: Human
    Sample Types: Cell Culture Lysates
    Applications: Western Blot
  13. SILAC Analysis Reveals Increased Secretion of Hemostasis-Related Factors by Senescent Cells
    Authors: CD Wiley, S Liu, C Limbad, AM Zawadzka, J Beck, M Demaria, R Artwood, F Alimirah, JA Lopez-Domi, C Kuehnemann, SR Danielson, N Basisty, HG Kasler, TR Oron, PY Desprez, SD Mooney, BW Gibson, B Schilling, J Campisi, P Kapahi
    Cell Rep, 2019-09-24;28(13):3329-3337.e5.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  14. Higher levels of TIMP-1 expression are associated with a poor prognosis in triple-negative breast cancer
    Authors: Guangcun Cheng, Xuemei Fan, Mingang Hao, Jinglong Wang, Xiaoming Zhou, Xueqing Sun
    Molecular Cancer
  15. Targeting TR4 nuclear receptor suppresses prostate cancer invasion via reduction of infiltrating macrophages with alteration of the TIMP-1/MMP2/MMP9 signals.
    Authors: Ding X, Yang D, Xia L, Chen B, Yu S, Niu Y, Wang M, Li G, Chang C
    Mol Cancer, 2015-01-27;14(0):16.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  16. Plasma levels of the MMP-9:TIMP-1 complex as prognostic biomarker in breast cancer: a retrospective study.
    Authors: Thorsen S, Christensen S, Wurtz S, Lundberg M, Nielsen B, Vinther L, Knowles M, Gee N, Fredriksson S, Moller S, Brunner N, Schrohl A, Stenvang J
    BMC Cancer, 2013-12-13;13(0):598.
    Species: Human
    Sample Types: Plasma
    Applications: Proximity Ligation Assay (PLA)
  17. Adipocytes promote ovarian cancer metastasis and provide energy for rapid tumor growth.
    Authors: Nieman KM, Kenny HA, Penicka CV, Ladanyi A, Buell-Gutbrod R, Zillhardt MR, Romero IL, Carey MS, Mills GB, Hotamisligil GS, Yamada SD, Peter ME, Gwin K, Lengyel E
    Nat. Med., 2011-10-30;17(11):1498-503.
    Species: Human
    Sample Types: In Vivo
    Applications: In Vivo
  18. Breast cancer cells induce cancer-associated fibroblasts to secrete hepatocyte growth factor to enhance breast tumorigenesis.
    Authors: Tyan SW, Kuo WH, Huang CK
    PLoS ONE, 2011-01-13;6(1):e15313.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  19. Primary human acute myelogenous leukemia cells release matrix metalloproteases and their inhibitors: release profile and pharmacological modulation.
    Authors: Reikvam H, Hatfield KJ, Oyan AM, Kalland KH, Kittang AO, Bruserud O
    Eur. J. Haematol., 2009-11-17;84(3):239-51.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  20. Fibroblast-conditioned media promote human sarcoma cell invasion.
    Authors: Bittner JG, Wilson M, Shah MB, Albo D, Feig BW, Wang TN
    Surgery, 2008-09-14;145(1):42-7.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  21. Coregulation of vascular tube stabilization by endothelial cell TIMP-2 and pericyte TIMP-3.
    Authors: Saunders WB, Bohnsack BL, Faske JB, Anthis NJ, Bayless KJ, Hirschi KK, Davis GE
    J. Cell Biol., 2006-10-09;175(1):179-91.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  22. Median raphe serotonergic neurons projecting to the interpeduncular nucleus control preference and aversion
    Authors: H Kawai, Y Bouchekiou, N Nishitani, K Niitani, S Izumi, H Morishita, C Andoh, Y Nagai, M Koda, M Hagiwara, K Toda, H Shirakawa, K Nagayasu, Y Ohmura, M Kondo, K Kaneda, M Yoshioka, S Kaneko
    Nature Communications, 2022-12-22;13(1):7708.
  23. Involvement of TIMP-1 in PECAM-1-mediated tumor dissemination
    Authors: Abraham V, Cao G, Parambath A et al.
    Int J Onc

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Human TIMP-1 Antibody
By Anonymous on 03/21/2022
Application: WB Sample Tested: mesenchymal stem cells derived extracellular vesicles Species: Human

Negative control: murine macrophages.
MSC-EV: mesenchymal stem cells derived extracellular vesicles

Western Blot Human TIMP-1 Antibody AF970
Human TIMP-1 Antibody
By Balaji Mahender on 12/06/2017
Application: ELISA Sample Tested: EDTA Plasma Species: Human