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Human TIMP-1 Quantikine ELISA Kit

Catalog #: DTM100
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Human TIMP-1 ELISA Standard Curve
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Summary Sample Values Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Human TIMP-1 Quantikine ELISA Kit Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
3.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (10 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL), Saliva (10 uL)
Sensitivity
0.08 ng/mL
Assay Range
0.2 - 10 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva)
Specificity
Natural and recombinant human TIMP-1
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.Cross-species reactivity not tested.
Interference
Interference observed with 1 or more available related molecules.

Sample Values

Serum/Plasma/Saliva - Samples from apparently healthy volunteers were evaluated for the presence of human TIMP-1 in this assay. No medical histories were available for the donors used in this study.

Sample TypeMean (ng/mL)Range (ng/mL)Standard Deviation (ng/mL)
Serum (n=60)19087-52472.1
Heparin plasma (n=60)8439-27933.1
EDTA plasma (n=60)9844-30435.4
Saliva (n=4)12146-20882.3

Cell Culture Supernates - Human peripheral blood mononuclear cells (5 x 106 cells/mL) were cultured in RPMI supplemented with 5% fetal bovine serum, 50 μM beta -mercaptoethanol, 2 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. The cells were cultured unstimulated or stimulated with 10 μg/mL PHA. Aliquots of the culture supernate were removed on days 1 and 5 and assayed for levels of natural human TIMP-1.

Condition Day 1 (ng/mL)Day 5 (ng/mL)
Unstimulated76163
Stimulated201274




Product Summary

The Quantikine Human TIMP-1 Immunoassay is a 3.5 hour solid phase ELISA designed to measure TIMP-1 concentrations in cell culture supernates, serum, plasma, and saliva. It contains NS0-expressed recombinant human TIMP-1 and antibodies raised against the recombinant factor. It has been shown to quantitate recombinant human TIMP-1 accurately. Results obtained using natural TIMP-1 showed linear curves that were parallel to the standard curve obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural TIMP-1.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean (ng/mL) 0.48 1.27 6.95 0.51 1.28 6.9
Standard Deviation 0.02 0.05 0.35 0.02 0.05 0.34
CV% 4.2 3.9 5 3.9 3.9 4.9

Recovery

The recovery of TIMP-1 spiked to three different levels in samples throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=5) 102 94-113
EDTA Plasma (n=5) 99 93-108
Heparin Plasma (n=5) 99 89-108
Saliva (n=4) 105 89-121
Serum (n=5) 98 87-102

Linearity

To assess the linearity of the assay, samples containing or spiked with high concentrations of TIMP-1 were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human TIMP-1 ELISA Linearity

Scientific Data

N/A Human TIMP-1 ELISA Standard Curve View Larger

Human TIMP-1 ELISA Standard Curve

Product Datasheets

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Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: TIMP-1

TIMPs-1 through -4 regulate the activity of zinc metalloproteases known as MMPs, ADAMs and ADAMTSs. Structurally, TIMPs contain two domains. The N-terminal domain binds to the active site of mature metalloproteases via a 1:1 non-covalent interaction, blocking access of substrates to the catalytic site. In addition, The C-terminal domain of TIMP-1 and TIMP-2 binds to the hemopexin- like domain of pro-MMP-9 and pro-MMP-2, respectively. The latter binding is essential for the cell surface activation of MMP-2 by MMP-14.

Long Name:
Tissue Inhibitors of Metalloproteinases 1
Entrez Gene IDs:
7076 (Human); 21857 (Mouse); 116510 (Rat)
Alternate Names:
CLGI; Collagenase inhibitor; collagenase inhibitor); EPATIMP-1; EPO; erythroid potentiating activity; Erythroid-potentiating activity; Fibroblast collagenase inhibitor; FLJ90373; HCI; metalloproteinase inhibitor 1; TIMP metallopeptidase inhibitor 1; TIMP1; TIMP-1; TIMPtissue inhibitor of metalloproteinase 1 (erythroid potentiating activity; Tissue inhibitor of metalloproteinases 1
&#9888; WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Assay Procedure

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
    3. 100 µL Assay Diluent
  3.   Add 100 µL of Assay Diluent to each well.
    4. 50 µL Standard, Control, or Sample
  4.   Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours on a horizontal orbital microplate shaker.
  5.   Aspirate each well and wash, repeating the process twice for a total of 3 washes.
    6. 200 µL Conjugate
  6.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 1 hour on the shaker.
  7.   Aspirate and wash 3 times.
    8. 200 µL Substrate Solution
  8.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.
    9. 50 µL Stop Solution
  9.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Citations for Human TIMP-1 Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

97 Citations: Showing 1 - 10
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  1. A metabolic-dysfunction associated steatotic liver acinus biomimetic induces pancreatic islet dysfunction in a coupled microphysiology system
    Authors: Aleman, J;Ravikumar, K;Wiegand, C;Schurdak, ME;Vernetti, L;Gavlock, D;Reese, C;DeBiasio, R;LaRocca, G;Angarita, YD;Gough, A;Soto-Gutierrez, A;Behari, J;Yechoor, V;Miedel, MT;Stern, AM;Banerjee, I;Taylor, DL;
    bioRxiv : the preprint server for biology
    Species: Human
    Sample Types: Tissue Homogenates
  2. Integrated Analysis of Transcriptome and Proteome of the Human Cornea and Aqueous Humor Reveal Novel Biomarkers for Corneal Endothelial Cell Dysfunction
    Authors: Moon, CE;Kim, CH;Jung, JH;Cho, YJ;Choi, KY;Han, K;Seo, KY;Lee, HK;Ji, YW;
    International journal of molecular sciences
    Species: Human
    Sample Types: Aqueous Humor
  3. Myeloma Microenvironmental TIMP1 Induces the Invasive Phenotype in Fibroblasts to Modulate Disease Progression
    Authors: R Ishihara, T Oda, Y Murakami, I Matsumura, S Watanabe, Y Asao, Y Masuda, N Gotoh, T Kasamatsu, H Takei, N Kobayashi, N Sasaki, T Saitoh, H Murakami, H Handa
    International Journal of Molecular Sciences, 2023-01-22;24(3):.
    Species: Human
    Sample Types: Plasma
  4. Enzymes of Fibrosis in Chronic Liver Disease
    Authors: I Tsomidis, G Notas, C Xidakis, A Voumvourak, DN Samonakis, M Koulentaki, E Kouroumali
    Biomedicines, 2022-12-08;10(12):.
    Species: Human
    Sample Types: Serum
  5. Estrogen Receptor Subtypes Elicit a Distinct Gene Expression Profile of Endothelial-Derived Factors Implicated in Atherosclerotic Plaque Vulnerability
    Authors: N Nasiri-Ans, E Spilioti, I Kyrou, V Kalotychou, A Chatzigeor, D Sanoudou, K Dahlman-Wr, HS Randeva, AG Papavassil, P Moutsatsou, E Kassi
    International Journal of Molecular Sciences, 2022-09-19;23(18):.
    Species: Human
    Sample Types: Cell Culture Supernates
  6. Changes in Microbiome Dominance Are Associated With Declining Lung Function and Fluctuating Inflammation in People With Cystic Fibrosis
    Authors: DL Frey, C Bridson, S Dittrich, SY Graeber, M Stahl, S Wege, F Herth, O Sommerburg, C Schultz, A Dalpke, MA Mall, S Boutin
    Frontiers in Microbiology, 2022-05-13;13(0):885822.
    Species: Human
    Sample Types: Sputum
  7. Molecular Biological Comparison of Dental Pulp- and Apical Papilla-Derived Stem Cells
    Authors: M Smeda, KM Galler, M Woelflick, A Rosendahl, C Moehle, B Lenhardt, W Buchalla, M Widbiller
    International Journal of Molecular Sciences, 2022-02-27;23(5):.
    Species: Human
    Sample Types: Cell Culture Supernates
  8. Derivation and validation of urinary TIMP-1 for the prediction of acute kidney injury and mortality in critically ill children
    Authors: H Huang, Q Lin, X Dai, J Chen, Z Bai, X Li, F Fang, Y Li
    Journal of Translational Medicine, 2022-02-23;20(1):102.
    Species: Human
    Sample Types: Urine
  9. Modulation by 17,20S(OH)2pD of Fibrosis-Related Mediators in Dermal Fibroblast Lines from Healthy Donors and from Patients with Systemic Sclerosis
    Authors: ML Brown Lobb, AT Slominski, KA Hasty, S Zhang, DD Miller, W Li, TK Kim, Z Janjetovic, RC Tuckey, IO Scott, LK Myers, AE Postlethwa
    International Journal of Molecular Sciences, 2021-12-29;23(1):.
    Species: Human
    Sample Types: Cell Culture Supernates
  10. Assessment and Prognosis in CSA-AKI Using Novel Kidney Injury Biomarkers: A Prospective Observational Study
    Authors: J Udzik, A Waszczyk, K Safranow, A Biskupski, K Majer, S Kwiatkowsk, E Kwiatkowsk
    Biology, 2021-08-24;10(9):.
    Species: Human
    Sample Types: Urine
  11. Zinc Administration and Improved Serum Markers of Hepatic Fibrosis in Patients with Autoimmune Hepatitis
    Authors: K Moriya, N Nishimura, T Namisaki, H Takaya, Y Sawada, H Kawaratani, K Kaji, N Shimozato, S Sato, M Furukawa, A Douhara, T Akahane, A Mitoro, J Yamao, H Yoshiji
    Journal of Clinical Medicine, 2021-06-02;10(11):.
    Species: Human
    Sample Types: Serum
  12. Level of Adiponectin, Leptin and Selected Matrix Metalloproteinases in Female Overweight Patients with Primary Gonarthrosis
    Authors: J Jarecki, T Ma?ecka-Ma, I Polkowska, B Potoczniak, E Kosior-Jar, I Szerb, E Tomaszewsk, M Gutbier, M Dobrzy?ski, T Blicharski
    Journal of Clinical Medicine, 2021-03-18;10(6):.
    Species: Human
    Sample Types: Serum
  13. Effect of vitamin D supplementation in patients with chronic hepatitis C after direct-acting antiviral treatment: a randomized, double-blind, placebo-controlled trial
    Authors: S Sriphoosan, K Thanapirom, SJ Kerr, S Suksawatam, P Thaimai, S Sittisomwo, K Sonsiri, N Srisoontho, N Teeratorn, N Tanpowpong, B Chaopathom, S Treepraser, Y Poovorawan, P Komolmit
    PeerJ, 2021-02-09;9(0):e10709.
    Species: Human
    Sample Types: Serum
  14. Matrix Metalloproteinases MMP-2 and MMP-9, Their Inhibitors TIMP-1 and TIMP-2, Vascular Endothelial Growth Factor and sVEGFR-2 as Predictive Markers of Ischemic Retinopathy in Patients with Systemic Sclerosis-Case Series Report
    Authors: A Waszczykow, M Podgórski, M Waszczykow, Z Gerlicz-Ko, P Jurowski
    Int J Mol Sci, 2020-11-18;21(22):.
    Species: Human
    Sample Types: Serum
  15. Aberrant recruitment of leukocytes defines poor wound healing in patients with recessive dystrophic epidermolysis bullosa
    Authors: T Phillips, L Huitema, R Cepeda, DL Cobos, RIM Perez, MS Garza, F Ringpfeil, B Dasgeb, J Uitto, JC Salas-Alan, V Alexeev, O Igoucheva
    J Dermatol Sci, 2020-10-17;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  16. Variations in Circulating Active MMP-9 Levels During Renal Replacement Therapy
    Authors: E Rodríguez-, JA Navarro-Ga, J Aceves-Rip, J Abarca-Zab, A Susmozas-S, T Bada-Bosch, E Hernández, E Mérida-Her, A Andrés, M Praga, M Fernández-, JM Aguado, J Segura, LM Ruilope, G Ruiz-Hurta
    Biomolecules, 2020-03-26;10(4):.
    Species: Human
    Sample Types: Plasma
  17. Elevated circulating metalloproteinase 7 predicts recurrent cardiovascular events in patients with carotid stenosis: a prospective cohort study
    Authors: D Moreno-Ajo, P Irimia, JA Rodríguez, MJ García-Vel, J López-Fida, L Fernández-, L Grochowitz, R Muñoz, P Domínguez, J Gállego-Cu, E Martínez-V
    BMC Cardiovasc Disord, 2020-02-26;20(1):93.
    Species: Human
    Sample Types: Serum
  18. Serum melatonin is inversely associated with matrix metalloproteinase-9 in oral squamous cell carcinoma
    Authors: AE Stanciu, A Zamfir-Chi, MM Stanciu, A Pantea-Sto, C Nitipir, DC Gheorghe
    Oncol Lett, 2020-02-13;19(4):3011-3020.
    Species: Human
    Sample Types: Serum
  19. Levels of Selected Matrix Metalloproteinases, Their Inhibitors in Saliva, and Oral Status in Juvenile Idiopathic Arthritis Patients vs. Healthy Controls
    Authors: A Kobus, J Bagi?ska, J ?api?ska-A, S ?awicki, A Kierklo
    Biomed Res Int, 2019-10-28;2019(0):7420345.
    Species: Human
    Sample Types: Saliva
  20. Basilar Artery Tortuosity Is Associated With White Matter Hyperintensities by TIMP-1
    Authors: DP Zhang, YF Peng, HL Zhang, JG Ma, M Zhao, S Yin, TT Wei
    Front Neurosci, 2019-08-14;13(0):836.
    Species: Human
    Sample Types: Plasma
  21. Effect of doxycyline in chronic obstructive pulmonary disease - An exploratory study
    Authors: B Singh, N Ghosh, D Saha, S Sarkar, P Bhattachar, K Chaudhury
    Pulm Pharmacol Ther, 2019-07-24;0(0):101831.
    Species: Human
    Sample Types: Plasma
  22. High serum levels of tissue inhibitor of matrix metalloproteinase-1 during the first week of a malignant middle cerebral artery infarction in non-surviving patients
    Authors: L Lorente, MM Martín, L Ramos, M Argueso, JJ Cáceres, J Solé-Violá, A Jiménez, JM Borreguero, AF González-R, J Orbe, JA Rodríguez, JA Páramo
    BMC Neurol, 2019-07-18;19(1):167.
    Species: Human
    Sample Types: Serum
  23. Effect of Peritoneal Dialysis on Serum Fibrosis Biomarkers in Patients with Refractory Congestive Heart Failure
    Authors: M Kunin, V Carmon, P Beckerman, D Dinour
    Int J Mol Sci, 2019-05-28;20(11):.
    Species: Human
    Sample Types: Plasma
  24. Expression and Concentration of Matrix Metalloproteinase 9 and Tissue Inhibitor of Matrix Metalloproteinases 1 in Laryngeal Squamous Cell Carcinoma
    Authors: M Matulka, A Konopka, B Mroczko, A Pryczynicz, A Kemona, M Groblewska, A Sieskiewic, E Olszewska
    Dis. Markers, 2019-04-18;2019(0):3136792.
    Species: Human
    Sample Types: Plasma
  25. Persistently high circulating tissue inhibitor of matrix metalloproteinase-1 levels in non-survivor brain trauma injury patients
    Authors: L Lorente, MM Martín, L Ramos, M Argueso, JJ Cáceres, J Solé-Violá, A Jiménez, JM Borreguero, AF González-R, J Orbe, JA Rodríguez, JA Páramo
    J Crit Care, 2019-02-19;51(0):117-121.
    Species: Human
    Sample Types: Serum
  26. Ginsenoside Rg1 Accelerates Paracrine Activity and Adipogenic Differentiation of Human Breast Adipose-Derived Stem Cells in a Dose-Dependent Manner In Vitro
    Authors: ZJ Liang, X Lu, DD Zhu, XL Yi, FX Wu, N He, C Tang, CY Wei, HM Li
    Cell Transplant, 2019-01-24;0(0):9636897198256.
    Species: Human
    Sample Types: Cell Culture Supernates
  27. Combination of Plasma Biomarkers and Clinical Data for the Detection of Myocardial Fibrosis or Aggravation of Heart Failure Symptoms in Heart Failure with Preserved Ejection Fraction Patients
    Authors: CK Wu, MM Su, YF Wu, JJ Hwang, LY Lin
    J Clin Med, 2018-11-08;7(11):.
    Species: Human
    Sample Types: Plasma
  28. Diagnostic power of VEGF, MMP-9 and TIMP-1 in patients with breast cancer. A multivariate statistical analysis with ROC curve
    Authors: M Zajkowska, E Gacuta, S Koz?owska, E Lubowicka, EK G?a?ewska, L Chrostek, M Szmitkowsk, P Paw?owski, M Zbucka-Kr?, S ?awicki
    Adv Med Sci, 2018-09-15;64(1):1-8.
    Species: Human
    Sample Types: Plasma
  29. The correlation of age and body mass index with the level of both protease MMP3 and anti-protease TIMP-1 among Indonesian patients with chronic obstructive pulmonary disease: a preliminary findings
    Authors: Mulyadi, Sunnati, M Azhary, F Yunus, F Nurwidya
    BMC Res Notes, 2018-08-02;11(1):551.
    Species: Human
    Sample Types: Saliva
  30. Identification of novel response and predictive biomarkers to Hsp90 inhibitors through mass spectrometry-based proteomic profiling of patient-derived prostate tumor explants
    Authors: EV Nguyen, MM Centenera, M Moldovan, R Das, S Irani, AD Vincent, H Chan, LG Horvath, DJ Lynn, R Daly, LM Butler
    Mol. Cell Proteomics, 2018-04-09;0(0):.
    Species: Human
    Sample Types: Complex Sample Type
  31. The role of pro-fibrotic biomarkers in paroxysmal and persistent atrial fibrillation
    Authors: AE Stanciu, RG Vatasescu, MM Stanciu, N Serdarevic, M Dorobantu
    Cytokine, 2018-01-08;103(0):63-68.
    Species: Human
    Sample Types: Serum
  32. MMP-8 and TIMP-1 are associated to periodontal inflammation in patients with rheumatoid arthritis under methotrexate immunosuppression - First results of a cross-sectional study
    Authors: G Schmalz, I Davarpanah, J Jäger, RF Mausberg, B Krohn-Grim, J Schmidt, R Haak, U Sack, D Ziebolz
    J Microbiol Immunol Infect, 2017-09-04;0(0):.
    Species: Human
    Sample Types: Serum
  33. Influence of ethnic background on left atrial markers of inflammation, endothelial function and tissue remodelling
    Authors: CD Ruediger, B John, S Kumar, HS Lim, G Rangnekar, KC Roberts-Th, GD Young, D Chase, P Sanders, SR Willoughby
    Indian Pacing Electrophysiol J, 2017-08-30;18(1):1-5.
    Species: Human
    Sample Types: Serum
  34. Collagen turnover biomarkers and systemic right ventricle remodeling in adults with previous atrial switch procedure for transposition of the great arteries
    Authors: M Lipczy?ska, P Szyma?ski, M Kumor, A Klisiewicz, P Hoffman
    PLoS ONE, 2017-08-02;12(8):e0180629.
    Species: Human
    Sample Types: Serum
  35. Cerebrospinal fluid matrix metalloproteinase 9 levels, blood-brain barrier permeability, and treatment outcome in tuberculous meningitis
    Authors: S Mailankody, GV Dangeti, R Soundraval, NM Joseph, J Mandal, TK Dutta, T Kadhiravan
    PLoS ONE, 2017-07-12;12(7):e0181262.
    Species: Human
    Sample Types: CSF
  36. TIMP1 and MMP9 are predictors of mortality in septic patients in the emergency department and intensive care unit unlike MMP9/TIMP1 ratio: Multivariate model
    Authors: ME Niño, SE Serrano, DC Niño, DM McCosham, ME Cardenas, VP Villareal, M Lopez, A Pazin-Filh, FA Jaimes, F Cunha, R Schulz, D Torres-Due
    PLoS ONE, 2017-02-13;12(2):e0171191.
    Species: Human
    Sample Types: Serum
  37. Plasma matrix metalloprotease 9 correlates with blood lymphocytosis, leukemic cell invasiveness, and prognosis in B-cell chronic lymphocytic leukemia
    Authors: M Gusella, C Bolzonella, R Paolini, E Rodella, L Bertolaso, C Scipioni, S Bellini, A Cuneo, F Pasini, E Ramazzina
    Tumour Biol, 2017-02-01;39(2):1010428317694.
    Species: Human
    Sample Types: Plasma
  38. Relationship between Serum Levels of Metalloproteinase-8 and Tissue Inhibitor of Metalloproteinases-1 and Exercise Test Results in Postmenopausal Women
    Authors: J Mieczkowsk, E Rutkowska, J Mosiewicz, B Mosiewicz
    Dis. Markers, 2016-12-26;2016(0):7169531.
    Species: Human
    Sample Types: Serum
  39. Targeting LOXL2 for cardiac interstitial fibrosis and heart failure treatment
    Nat Commun, 2016-12-14;7(0):13710.
    Species: Human
    Sample Types: Plasma
  40. TIMP-1 Inhibits Apoptosis in Lung Adenocarcinoma Cells via Interaction with Bcl-2.
    Authors: Nalluri S, Ghoshal-Gupta S, Kutiyanawalla A, Gayatri S, Lee B, Jiwani S, Rojiani A, Rojiani M
    PLoS ONE, 2015-09-14;10(9):e0137673.
    Species: Human
    Sample Types: Cell Culture Supernates
  41. Metallothionein-3 Increases Triple-Negative Breast Cancer Cell Invasiveness via Induction of Metalloproteinase Expression.
    Authors: Kmiecik A, Pula B, Suchanski J, Olbromski M, Gomulkiewicz A, Owczarek T, Kruczak A, Ambicka A, Rys J, Ugorski M, Podhorska-Okolow M, Dziegiel P
    PLoS ONE, 2015-05-01;10(5):e0124865.
    Species: Human
    Sample Types: Cell Culture Supernates
  42. Exogenous IFN-beta regulates the RANKL-c-Fos-IFN-beta signaling pathway in the collagen antibody-induced arthritis model.
    Authors: Zhao R, Chen N, Zhou X, Miao P, Hu C, Qian L, Yu Q, Zhang J, Nie H, Chen X, Li P, Xu R, Xiao L, Zhang X, Liu J, Zhang D
    J Transl Med, 2014-12-10;12(1):330.
    Species: Human
    Sample Types: Serum
  43. Low-density lipoprotein receptor-related protein-1 mediates endocytic clearance of tissue inhibitor of metalloproteinases-1 and promotes its cytokine-like activities.
    Authors: Thevenard J, Verzeaux L, Devy J, Etique N, Jeanne A, Schneider C, Hachet C, Ferracci G, David M, Martiny L, Charpentier E, Khrestchatisky M, Rivera S, Dedieu S, Emonard H
    PLoS ONE, 2014-07-30;9(7):e103839.
    Species: Hamster
    Sample Types: Cell Culture Supernates
  44. Identification and characterization of PDGFRalpha+ mesenchymal progenitors in human skeletal muscle.
    Authors: Uezumi A, Fukada S, Yamamoto N, Ikemoto-Uezumi M, Nakatani M, Morita M, Yamaguchi A, Yamada H, Nishino I, Hamada Y, Tsuchida K
    Cell Death Dis, 2014-04-17;5(0):e1186.
    Species: Human
    Sample Types: Cell Culture Supernates
  45. Association between serum tissue inhibitor of matrix metalloproteinase-1 levels and mortality in patients with severe brain trauma injury.
    Authors: Lorente L, Martin M, Lopez P, Ramos L, Blanquer J, Caceres J, Sole-Violan J, Solera J, Cabrera J, Argueso M, Ortiz R, Mora M, Lubillo S, Jimenez A, Borreguero-Leon J, Gonzalez A, Orbe J, Rodriguez J, Paramo J
    PLoS ONE, 2014-04-11;9(4):e94370.
    Species: Human
    Sample Types: Serum
  46. Interleukin-6 and matrix metalloproteinase expression in the subretinal fluid during proliferative vitreoretinopathy: correlation with extent, duration of RRD and PVR grade.
    Authors: Symeonidis C, Papakonstantinou E, Androudi S, Tsaousis KT, Tsinopoulos I, Brazitikos P, Karakiulakis G, Diza E, Dimitrakos SA
    Cytokine, 2012-05-10;59(1):184-90.
    Species: Human
    Sample Types: Subretinal Fluid
  47. Validation and quality control of ELISAs for the use with human saliva samples.
    Authors: Jaedicke KM, Taylor JJ, Preshaw PM
    J. Immunol. Methods, 2012-01-28;377(1):62-5.
    Species: Human
    Sample Types: Saliva
  48. Prognostic significance of TIMP-1 in non-small cell lung cancer.
    Authors: Pesta M, Kulda V, Kucera R
    Anticancer Res., 2011-11-01;31(11):4031-8.
    Species: Human
    Sample Types: Plasma
  49. Early Elevation of Matrix Metalloproteinase-8 and -9 in Pediatric ARDS Is Associated with an Increased Risk of Prolonged Mechanical Ventilation.
    Authors: Kong MY, Li Y, Oster R, Gaggar A, Clancy JP
    PLoS ONE, 2011-08-03;6(8):e22596.
    Species: Human
    Sample Types: Tissue Secretion
  50. TLR4 Protein Contributes to Cigarette Smoke-induced Matrix Metalloproteinase-1 (MMP-1) Expression in Chronic Obstructive Pulmonary Disease.
    Authors: Geraghty P, Dabo AJ, D'Armiento J
    J. Biol. Chem., 2011-07-05;286(34):30211-8.
    Species: Human
    Sample Types: Cell Culture Supernates
  51. Increased levels of leukocyte-derived MMP-9 in patients with stable angina pectoris.
    Authors: Jonasson S, Lundberg A, Kalvegren H, Bergstrom I, Szymanowski A, Jonasson L
    PLoS ONE, 2011-04-29;6(4):e19340.
    Species: Human
    Sample Types: Serum
  52. Plasma markers for identifying patients with metastatic melanoma.
    Authors: Kluger HM, Hoyt K, Bacchiocchi A, Mayer T, Kirsch J, Kluger Y, Sznol M, Ariyan S, Molinaro A, Halaban R
    Clin. Cancer Res., 2011-04-12;17(8):2417-25.
    Species: Human
    Sample Types: Plasma
  53. Tissue inhibitor of matrix metalloproteinase 1 (TIMP1) controls adipogenesis in obesity in mice and in humans.
    Authors: Meissburger B, Stachorski L, Roder E, Rudofsky G, Wolfrum C
    Diabetologia, 2011-03-25;54(6):1468-79.
    Species: Human
    Sample Types: Serum
  54. Interleukin-6 and the matrix metalloproteinase response in the vitreous during proliferative vitreoretinopathy.
    Authors: Symeonidis C, Papakonstantinou E, Androudi S, Rotsos T, Diza E, Brazitikos P, Karakiulakis G, Dimitrakos SA
    Cytokine, 2011-02-25;54(2):212-7.
    Species: Human
    Sample Types: Vitreous Humor
  55. Biomarkers of extracellular matrix metabolism (MMP-9 and TIMP-1) and risk of stroke, myocardial infarction, and cause-specific mortality: cohort study.
    Authors: Hansson J, Vasan RS, Arnlov J, Ingelsson E, Lind L, Larsson A, Michaelsson K, Sundstrom J
    PLoS ONE, 2011-01-19;6(1):e16185.
    Species: Human
    Sample Types: Serum
  56. A multi-analyte serum test for the detection of non-small cell lung cancer.
    Authors: Farlow EC, Vercillo MS, Coon JS, Basu S, Kim AW, Faber LP, Warren WH, Bonomi P, Liptay MJ, Borgia JA
    Br. J. Cancer, 2010-09-21;103(8):1221-8.
    Species: Human
    Sample Types: Serum
  57. Plasma haemoxygenase-1 in coronary artery disease. A comparison with angiogenin, matrix metalloproteinase-9, tissue inhibitor of metalloproteinase-1 and vascular endothelial growth factor.
    Authors: Idriss NK, Lip GY, Balakrishnan B
    Thromb. Haemost., 2010-09-13;104(5):1029-37.
    Species: Human
    Sample Types: Plasma
  58. The effect of aspirin desensitization on novel biomarkers in aspirin-exacerbated respiratory diseases.
    Authors: Katial RK, Strand M, Prasertsuntarasai T, Leung R, Zheng W, Alam R
    J. Allergy Clin. Immunol., 2010-08-21;126(4):738-44.
    Species: Human
    Sample Types: Sputum
  59. The diagnostic value of matrix metalloproteinase 9 (MMP-9) and tissue inhibitor of matrix metalloproteinases 1 (TIMP-1) determination in the sera of colorectal adenoma and cancer patients.
    Authors: Mroczko B, Groblewska M, Okulczyk B, Kedra B, Szmitkowski M
    Int J Colorectal Dis, 2010-06-17;25(10):1177-84.
    Species: Human
    Sample Types: Serum
  60. Plasma tissue inhibitor of matrix metalloproteinase-1 (TIMP-1): an independent predictor of poor response to cardiac resynchronization therapy.
    Authors: Tolosana JM, Mont L, Sitges M, Berruezo A, Delgado V, Vidal B, Tamborero D, Morales M, Batlle M, Roig E, Castel MA, Perez-Villa F, Godoy M, Brugada J
    Eur. J. Heart Fail., 2010-04-01;12(5):492-8.
    Species: Human
    Sample Types: Plasma
  61. High matrix metalloproteinase production correlates with immune activation and leukocyte migration in leprosy reactional lesions.
    Authors: Teles RM, Teles RB, Amadeu TP, Moura DF, Mendonca-Lima L, Ferreira H, Santos IM, Nery JA, Sarno EN, Sampaio EP
    Infect. Immun., 2009-12-14;78(3):1012-21.
    Species: Human
    Sample Types: Serum
  62. Integrated proteomic analysis of human cancer cells and plasma from tumor bearing mice for ovarian cancer biomarker discovery.
    Authors: Pitteri SJ, JeBailey L, Faca VM, Thorpe JD, Silva MA, Ireton RC, Horton MB, Wang H, Pruitt LC, Zhang Q, Cheng KH, Urban N, Hanash SM, Dinulescu DM
    PLoS ONE, 2009-11-19;4(11):e7916.
    Species: Human
    Sample Types: Plasma
  63. Primary human acute myelogenous leukemia cells release matrix metalloproteases and their inhibitors: release profile and pharmacological modulation.
    Authors: Reikvam H, Hatfield KJ, Oyan AM, Kalland KH, Kittang AO, Bruserud O
    Eur. J. Haematol., 2009-11-17;84(3):239-51.
    Species: Human
    Sample Types: Cell Culture Supernates
  64. Matrix metalloproteinase-9, -10, and tissue inhibitor of matrix metalloproteinases-1 blood levels as biomarkers of severity and mortality in sepsis.
    Authors: Lorente L, Martin MM, Labarta L, Diaz C, Sole-Violan J, Blanquer J, Orbe J, Rodriguez JA, Jimenez A, Borreguero-Leon JM, Belmonte F, Medina JC, Lliminana MC, Ferrer-Aguero JM, Ferreres J, Mora ML, Lubillo S, Sanchez M, Barrios Y, Sierra A, Paramo JA
    Crit Care, 2009-10-02;13(5):R158.
    Species: Human
    Sample Types: Serum
  65. Serum matrix metalloproteinases in adult CF patients: Relation to pulmonary exacerbation.
    Authors: Roderfeld M, Rath T, Schulz R, Seeger W, Tschuschner A, Graf J, Roeb E
    J. Cyst. Fibros., 2009-07-14;8(5):338-47.
    Species: Human
    Sample Types: Serum
  66. Salbutamol up-regulates matrix metalloproteinase-9 in the alveolar space in the acute respiratory distress syndrome.
    Authors: O'Kane CM, McKeown SW, Perkins GD, Bassford CR, Gao F, Thickett DR, McAuley DF
    Crit. Care Med., 2009-07-01;37(7):2242-9.
    Species: Human
    Sample Types: BALF
  67. Gingival fibroblast inhibits MMP-7: evaluation in an ex vivo aorta model.
    Authors: Gogly B, Fournier B, Couty L, Naveau A, Brasselet C, Durand E, Coulomb B, Lafont A
    J. Mol. Cell. Cardiol., 2009-05-03;47(2):296-303.
    Species: Rabbit
    Sample Types: Cell Culture Supernates
  68. Immunomodulation by chronobiologically-based glucocorticoids treatment for multiple sclerosis relapses.
    Authors: Glass-Marmor L, Paperna T, Galboiz Y, Miller A
    J. Neuroimmunol., 2009-03-28;210(1):124-7.
    Species: Human
    Sample Types: Serum
  69. Matrix metalloproteinase activity in pediatric acute lung injury.
    Authors: Kong MY, Gaggar A, Li Y
    Int J Med Sci, 2008-12-16;6(1):9-17.
    Species: Human
    Sample Types: Tissue Secretion
  70. Imbalance of tissue inhibitors of metalloproteinases (TIMP) - 1 and - 4 serum levels, in patients with inflammatory bowel disease.
    Authors: Kapsoritakis AN, Kapsoritaki AI, Davidi IP, Lotis VD, Manolakis AC, Mylonis PI, Theodoridou AT, Germenis AE, Potamianos SP
    BMC Gastroenterol, 2008-11-26;8(1):55.
    Species: Human
    Sample Types: Serum
  71. Activation of the osteopontin/matrix metalloproteinase-9 pathway correlates with prostate cancer progression.
    Authors: Castellano G, Malaponte G, Mazzarino MC, Figini M, Marchese F, Gangemi P, Travali S, Stivala F, Canevari S, Libra M
    Clin. Cancer Res., 2008-11-15;14(22):7470-80.
    Species: Human
    Sample Types: Plasma
  72. MMP expression and abnormal lung permeability are important determinants of outcome in IPF.
    Authors: McKeown S, Richter AG, O'Kane C, O'Kane C, McAuley DF, Thickett DR
    Eur. Respir. J., 2008-10-01;33(1):77-84.
    Species: Human
    Sample Types: BALF
  73. Protective effects of butanol fraction from Betula Platyphyla var. japonica on cartilage alterations in a rabbit collagenase-induced osteoarthritis.
    Authors: Huh JE, Baek YH, Kim YJ, Lee JD, Choi DY, Park DS
    J Ethnopharmacol, 2008-09-04;123(3):515-21.
    Species: Rabbit
    Sample Types: Synovial Fluid
  74. Gene transfer of matrix metalloproteinase-9 induces tumor regression of breast cancer in vivo.
    Authors: Bendrik C, Robertson J, Gauldie J, Dabrosin C
    Cancer Res., 2008-05-01;68(9):3405-12.
    Species: Human
    Sample Types: Tissue Homogenates
  75. Impact of acute exposure to tobacco smoke on gelatinases in the bronchoalveolar space.
    Authors: Glader P, Eldh B, Bozinovski S, Andelid K, Sjostrand M, Malmhall C, Anderson GP, Riise GC, Qvarfordt I, Linden A
    Eur. Respir. J., 2008-04-16;32(3):644-50.
    Species: Human
    Sample Types: BALF
  76. Use of an immunoaffinity-mass spectrometry-based approach for the quantification of protein biomarkers from serum samples of lung cancer patients.
    Authors: Nicol GR, Han M, Kim J, Birse CE, Brand E, Nguyen A, Mesri M, FitzHugh W, Kaminker P, Moore PA, Ruben SM, He T
    Mol. Cell Proteomics, 2008-04-03;7(10):1974-82.
    Species: Human
    Sample Types: Serum
  77. Is metalloproteinase-7 specific for idiopathic pulmonary fibrosis?
    Authors: Huh JW, Kim DS, Oh YM, Shim TS, Lim CM, Lee SD, Koh Y, Kim WS, Kim WD, Kim KR
    Chest, 2007-12-10;133(5):1101-6.
    Species: Human
    Sample Types: BALF
  78. Airway remodeling and lack of bronchodilator response in steroid-resistant asthma.
    Authors: Goleva E, Hauk PJ, Boguniewicz J, Martin RJ, Leung DY
    J. Allergy Clin. Immunol., 2007-09-27;120(5):1065-72.
    Species: Human
    Sample Types: BALF
  79. Tissue engineering for full-thickness burns: a dermal substitute from bench to bedside.
    Authors: Kellouche S, Martin C, Korb G, Rezzonico R, Bouard D, Benbunan M, Dubertret L, Soler C, Legrand C, Dosquet C
    Biochem. Biophys. Res. Commun., 2007-09-04;363(3):472-8.
    Species: Human
    Sample Types: Cell Culture Supernates
  80. Actinobacillus actinomycetemcomitans lipopolysaccharide regulates matrix metalloproteinase, tissue inhibitors of matrix metalloproteinase, and plasminogen activator production by human gingival fibroblasts: a potential role in connective tissue destruction.
    Authors: Bodet C, Andrian E, Tanabe S, Grenier D
    J. Cell. Physiol., 2007-07-01;212(1):189-94.
    Species: Human
    Sample Types: Cell Culture Supernates
  81. Matrix metalloproteinase-2 dysregulation in type 1 diabetes.
    Authors: Thrailkill KM, Bunn RC, Moreau CS, Cockrell GE, Simpson PM, Coleman HN, Frindik JP, Kemp SF, Fowlkes JL
    Diabetes Care, 2007-06-11;30(9):2321-6.
    Species: Human
    Sample Types: Plasma
  82. Borrelia burgdorferi-induced monocyte chemoattractant protein-1 production in vivo and in vitro.
    Authors: Zhao Z, McCloud B, Fleming R, Klempner MS
    Biochem. Biophys. Res. Commun., 2007-05-02;358(2):528-33.
    Species: Human
    Sample Types: Tissue Homogenates
  83. Oncostatin M induces angiogenesis and cartilage degradation in rheumatoid arthritis synovial tissue and human cartilage cocultures.
    Authors: Fearon U, Mullan R, Markham T, Connolly M, Sullivan S, Poole AR, FitzGerald O, Bresnihan B, Veale DJ
    Arthritis Rheum., 2006-10-01;54(10):3152-62.
    Species: Human
    Sample Types: Cell Culture Supernates
  84. Cell expression of MMP-1 and TIMP-1 in co-cultures of human gingival fibroblasts and monocytes: the involvement of ICAM-1.
    Authors: Domeij H, Modeer T, Quezada HC, Yucel-Lindberg T
    Biochem. Biophys. Res. Commun., 2005-11-02;338(4):1825-33.
    Species: Human
    Sample Types: Cell Culture Supernates
  85. A comparison of airway and serum matrix metalloproteinase-9 activity among normal subjects, asthmatic patients, and patients with asthmatic mucus hypersecretion.
    Authors: Ko FW, Diba C, Roth M, McKay K, Johnson PR, Salome C, King GG
    Chest, 2005-06-01;127(6):1919-27.
    Species: Human
    Sample Types: Serum
  86. Differential proteolytic enzyme activity in eosinophilic and neutrophilic asthma.
    Authors: Simpson JL, Scott RJ, Boyle MJ, Gibson PG
    Am. J. Respir. Crit. Care Med., 2005-05-18;172(5):559-65.
    Species: Human
    Sample Types: Sputum
  87. Plasma storage at -80 degrees C does not protect matrix metalloproteinase-9 from degradation.
    Authors: Rouy D, Ernens I, Jeanty C, Wagner DR
    Anal. Biochem., 2005-03-15;338(2):294-8.
    Species: Human
    Sample Types: Plasma
  88. Induced sputum matrix metalloproteinase-9 correlates with lung function and airway inflammation in children with cystic fibrosis.
    Authors: Sagel SD, Kapsner RK, Osberg I
    Pediatr. Pulmonol., 2005-03-01;39(3):224-32.
    Species: Human
    Sample Types: Sputum
  89. Induction of host matrix metalloproteinases by Borrelia burgdorferi differs in human and murine lyme arthritis.
    Authors: Behera AK, Hildebrand E, Scagliotti J, Steere AC, Hu LT
    Infect. Immun., 2005-01-01;73(1):126-34.
    Species: Human
    Sample Types: Synovial Fluid
  90. Plasma MMP-2 and MMP-9 and their inhibitors TIMP-1 and TIMP-2 during human orthotopic liver transplantation. The effect of aprotinin and the relation to ischemia/reperfusion injury.
    Authors: Kuyvenhoven JP, Molenaar IQ, Verspaget HW, Veldman MG, Palareti G, Legnani C, Moolenburgh SE, Terpstra OT, Lamers CB, van Hoek B, Porte RJ
    Thromb. Haemost., 2004-03-01;91(3):506-13.
    Species: Human
    Sample Types: Plasma
  91. Determination of the serum matrix metalloproteinase-9 (MMP-9) and tissue inhibitor of matrix metalloproteinase-1 (TIMP-1) in patients with either advanced small-cell lung cancer or non-small-cell lung cancer prior to treatment.
    Authors: Jumper C, Cobos E, Lox C
    Respir Med, 2004-02-01;98(2):173-7.
    Species: Human
    Sample Types: Serum
  92. Anti-inflammatory cytokine profile in acute coronary syndromes: behavior of interleukin-10 in association with serum metalloproteinases and proinflammatory cytokines.
    Authors: Tziakas DN, Chalikias GK, Hatzinikolaou HI, Parissis JT, Papadopoulos ED, Trypsianis GA, Papadopoulou E, Tentes IK, Karas SM, Hatseras DI
    Int. J. Cardiol., 2003-12-01;92(2):169-75.
    Species: Human
    Sample Types: Serum
  93. Matrix metalloproteinases and tissue inhibitors of metalloproteinases in sera and tissue of patients with Dupuytren's disease.
    Authors: Ulrich D, Hrynyschyn K, Pallua N
    Plast. Reconstr. Surg., 2003-10-01;112(5):1279-86.
    Species: Human
    Sample Types: Serum
  94. Matrix metalloproteinases production in malignant pleural effusions after talc pleurodesis.
    Authors: D&amp;apos;Agostino P
    Clin. Exp. Immunol., 2003-10-01;134(1):138-42.
    Species: Human
    Sample Types: Pleural Effusion
  95. Induced sputum-retrieved matrix metalloproteinase 9 and tissue metalloproteinase inhibitor 1 in granulomatous diseases.
    Authors: Fireman E, Kraiem Z, Sade O, Greif J, Fireman Z
    Clin. Exp. Immunol., 2002-11-01;130(2):331-7.
    Species: Human
    Sample Types: Saliva
  96. Matrix metalloproteinase-9, but not tissue inhibitor of matrix metalloproteinase-1, increases in the sputum from allergic asthmatic patients after allergen challenge.
    Authors: Cataldo DD, Bettiol J, Noel A, Bartsch P, Foidart JM, Louis R
    Chest, 2002-11-01;122(5):1553-9.
    Species: Human
    Sample Types: Sputum
  97. Matrix metalloproteinase-9 and tissue inhibitor of matrix metalloproteinase-1: a possible role in the pathogenesis of endometriosis.
    Authors: Szamatowicz J, Laudanski P, Tomaszewska I
    Hum. Reprod., 2002-02-01;17(2):284-8.
    Species: Human
    Sample Types: Peritoneal Fluid

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Human TIMP-1 Quantikine ELISA Kit
By Anonymous on 11/25/2022
Sample Tested: Serum
Human TIMP-1 Quantikine ELISA Kit
By Anonymous on 03/15/2022
Sample Tested: Urine

Urine was diluted 2-fold, 4-fold, and 8-fold. Two of 8 samples had a reference value below range, 5/8 samples were in range at the 4-fold dilution, and 4/8 samples were in range at the 8-fold dilution. The samples that were out of range were all BLQ. Despite some samples being BLQ at the lower dilutions, most of the samples showed good dilution linearity with %Recoveries within 80-120%.

Human TIMP-1 Quantikine ELISA Kit DTM100
Human TIMP-1 Quantikine ELISA Kit
By Anonymous on 06/07/2021
Sample Tested: Serum

sample dilution 1:3

Human TIMP-1 Quantikine ELISA Kit DTM100
Human TIMP-1 Quantikine ELISA Kit
By Anonymous on 07/17/2020
Sample Tested: Serum and Plasma
Human TIMP-1 Quantikine ELISA Kit DTM100