Human TIMP-2 Quantikine ELISA Kit Summary
Product Summary
Precision
Cell Culture Supernates, Saliva
Intra-Assay Precision | Inter-Assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 20 | 20 | 20 | 40 | 40 | 40 |
Mean (ng/mL) | 1.01 | 2.9 | 5.26 | 1.01 | 2.79 | 5.27 |
Standard Deviation | 0.042 | 0.173 | 0.344 | 0.079 | 0.188 | 0.326 |
CV% | 4.2 | 6 | 6.5 | 7.8 | 6.7 | 6.2 |
Serum, EDTA Plasma, Heparin Plasma
Intra-Assay Precision | Inter-Assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 20 | 20 | 20 | 40 | 40 | 40 |
Mean (ng/mL) | 1.23 | 3.45 | 6.09 | 1.26 | 3.45 | 6.38 |
Standard Deviation | 0.054 | 0.116 | 0.181 | 0.086 | 0.197 | 0.467 |
CV% | 4.4 | 3.4 | 3 | 6.8 | 5.7 | 7.3 |
Recovery
The recovery of TIMP-2 spiked to three different levels throughout the range of the assay was evaluated.
Sample Type | Average % Recovery | Range % |
---|---|---|
Cell Culture Media (n=4) | 95 | 81-108 |
Linearity
Scientific Data
Product Datasheets
Preparation and Storage
Background: TIMP-2
TIMPs-1 through -4 regulate the activity of zinc metalloproteases known as MMPs, ADAMs and ADAMTSs. Structurally, TIMPs contain two domains. The N-terminal domain binds to the active site of mature metalloproteases via a 1:1 non-covalent interaction, blocking access of substrates to the catalytic site. In addition, The C-terminal domain of TIMP-1 and TIMP-2 binds to the hemopexin- like domain of pro-MMP-9 and pro-MMP-2, respectively. The latter binding is essential for the cell surface activation of MMP-2 by MMP-14.
Assay Procedure
Refer to the product- Prepare all reagents, standard dilutions, and samples as directed in the product insert.
- Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
- Add 100 µL of Assay Diluent to each well.
- Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours on a horizontal orbital microplate shaker.
- Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.
- Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours on the shaker.
- Aspirate and wash 4 times.
- Add 200 µL Substrate Solution to each well.
- For Cell Culture Supernate & Saliva Samples: Incubate at room temperature for 20 minutes on the benchtop. PROTECT FROM LIGHT.
For Serum & Plasma Samples: Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT. - Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
Citations for Human TIMP-2 Quantikine ELISA Kit
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Urinary beta-2 microglobulin increases whereas TIMP-2 and IGFBP7 decline after unilateral nephrectomy in healthy kidney donors
Authors: de Rooij, ENM;Hoogeveen, EK;Romijn, FPHTM;van der Kooy, SW;Veighey, KV;Dekker, FW;van Kooten, C;Cobbaert, CM;de Fijter, JW;
Scientific reports
Species: Human
Sample Types: Urine
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Enzymes of Fibrosis in Chronic Liver Disease
Authors: I Tsomidis, G Notas, C Xidakis, A Voumvourak, DN Samonakis, M Koulentaki, E Kouroumali
Biomedicines, 2022-12-08;10(12):.
Species: Human
Sample Types: Serum
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Estrogen Receptor Subtypes Elicit a Distinct Gene Expression Profile of Endothelial-Derived Factors Implicated in Atherosclerotic Plaque Vulnerability
Authors: N Nasiri-Ans, E Spilioti, I Kyrou, V Kalotychou, A Chatzigeor, D Sanoudou, K Dahlman-Wr, HS Randeva, AG Papavassil, P Moutsatsou, E Kassi
International Journal of Molecular Sciences, 2022-09-19;23(18):.
Species: Human
Sample Types: Cell Culture Supernates
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Circulating biomarker correlates of left atrial size and myocardial extracellular volume fraction among persons living with and without HIV
Authors: TE Peterson, C Landon, SA Haberlen, F Bhondoekha, MW Plankey, FJ Palella, DA Piggott, JB Margolick, TT Brown, WS Post, KC Wu
BMC cardiovascular disorders, 2022-09-03;22(1):393.
Species: Human
Sample Types: Serum
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The ASIC3-M-CSF-M2 macrophage-positive feedback loop modulates fibroblast-to-myofibroblast differentiation in skin fibrosis pathogenesis
Authors: JJ Wu, ZL Sun, SY Liu, ZH Chen, ZD Yuan, ML Zou, YY Teng, YY Li, DY Guo, FL Yuan
Cell Death & Disease, 2022-06-06;13(6):527.
Species: Human
Sample Types: Cell Culture Supernates
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Combining renal cell arrest and damage biomarkers to predict progressive AKI in patient with sepsis
Authors: X Tao, C Chen, W Luo, J Zhou, J Tian, X Yang, FF Hou
Bmc Nephrology, 2021-12-15;22(1):415.
Species: Human
Sample Types: Urine
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Zinc Administration and Improved Serum Markers of Hepatic Fibrosis in Patients with Autoimmune Hepatitis
Authors: K Moriya, N Nishimura, T Namisaki, H Takaya, Y Sawada, H Kawaratani, K Kaji, N Shimozato, S Sato, M Furukawa, A Douhara, T Akahane, A Mitoro, J Yamao, H Yoshiji
Journal of Clinical Medicine, 2021-06-02;10(11):.
Species: Human
Sample Types: Serum
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The predictive value of TIMP-2 and IGFBP7 for kidney failure and 30-day mortality after elective cardiac surgery
Authors: K Esmeijer, A Schoe, LR Ruhaak, EK Hoogeveen, D Soonawala, FPHTM Romijn, MR Shirzada, JT van Dissel, CM Cobbaert, JW de Fijter
Scientific Reports, 2021-01-13;11(1):1071.
Species: Human
Sample Types: Urine
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Matrix Metalloproteinases MMP-2 and MMP-9, Their Inhibitors TIMP-1 and TIMP-2, Vascular Endothelial Growth Factor and sVEGFR-2 as Predictive Markers of Ischemic Retinopathy in Patients with Systemic Sclerosis-Case Series Report
Authors: A Waszczykow, M Podgórski, M Waszczykow, Z Gerlicz-Ko, P Jurowski
Int J Mol Sci, 2020-11-18;21(22):.
Species: Human
Sample Types: Serum
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Cdc42-Dependent Transfer of mir301 from Breast Cancer-Derived Extracellular Vesicles Regulates the Matrix Modulating Ability of Astrocytes at the Blood-Brain Barrier
Authors: G Morad, CC Daisy, HH Otu, TA Libermann, ST Dillon, MA Moses
Int J Mol Sci, 2020-05-28;21(11):.
Species: Mouse
Sample Types: Cell Culture Supernates
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Serum melatonin is inversely associated with matrix metalloproteinase-9 in oral squamous cell carcinoma
Authors: AE Stanciu, A Zamfir-Chi, MM Stanciu, A Pantea-Sto, C Nitipir, DC Gheorghe
Oncol Lett, 2020-02-13;19(4):3011-3020.
Species: Human
Sample Types: Serum
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Levels of Selected Matrix Metalloproteinases, Their Inhibitors in Saliva, and Oral Status in Juvenile Idiopathic Arthritis Patients vs. Healthy Controls
Authors: A Kobus, J Bagi?ska, J ?api?ska-A, S ?awicki, A Kierklo
Biomed Res Int, 2019-10-28;2019(0):7420345.
Species: Human
Sample Types: Saliva
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Basilar Artery Tortuosity Is Associated With White Matter Hyperintensities by TIMP-1
Authors: DP Zhang, YF Peng, HL Zhang, JG Ma, M Zhao, S Yin, TT Wei
Front Neurosci, 2019-08-14;13(0):836.
Species: Human
Sample Types: Plasma
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Urinary Tissue Inhibitor of�Metalloproteinases-2 and Insulin-Like Growth Factor-Binding Protein 7 Do Not Correlate With Disease Severity in ADPKD Patients
Authors: SEI Dekker, LR Ruhaak, FPHTM Romijn, E Meijer, CM Cobbaert, JW de Fijter, D Soonawala
Kidney Int Rep, 2019-03-22;4(6):833-841.
Species: Human
Sample Types: Urine
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A potential key mechanism in ascending aortic aneurysm development: Detection of a linear relationship between MMP-14/TIMP-2 ratio and active MMP-2
Authors: R Schmitt, A Tscheuschl, P Laschinski, X Uffelmann, P Discher, J Fuchs, M Kreibich, R Peyronnet, FA Kari
PLoS ONE, 2019-02-22;14(2):e0212859.
Species: Human
Sample Types: Tissue Homogenates
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Matrix metalloproteinase 2 (MMP-2) and its tissue inhibitor 2 (TIMP-2) in pancreatic cancer (PC)
Authors: M ?ukaszewic, M Gryko, S P?czek, M Szmitkowsk, B K?dra, B Mroczko
Oncotarget, 2019-01-08;10(3):395-403.
Species: Human
Sample Types: Serum
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Anti-cancer effect of dung beetle glycosaminoglycans on melanoma
Authors: MY Ahn, BJ Kim, HJ Kim, JM Jin, HJ Yoon, JS Hwang, KK Park
BMC Cancer, 2019-01-05;19(1):9.
Species: Human
Sample Types: Cell Culture Supernates
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Combination of Plasma Biomarkers and Clinical Data for the Detection of Myocardial Fibrosis or Aggravation of Heart Failure Symptoms in Heart Failure with Preserved Ejection Fraction Patients
Authors: CK Wu, MM Su, YF Wu, JJ Hwang, LY Lin
J Clin Med, 2018-11-08;7(11):.
Species: Human
Sample Types: Plasma
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Human umbilical cord plasma proteins revitalize hippocampal function in aged mice
Authors: JM Castellano, KI Mosher, RJ Abbey, AA McBride, ML James, D Berdnik, JC Shen, B Zou, XS Xie, M Tingle, IV Hinkson, MS Angst, T Wyss-Coray
Nature, 2017-04-19;544(7651):488-492.
Species: Human
Sample Types: Plasma
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TIMP1 and MMP9 are predictors of mortality in septic patients in the emergency department and intensive care unit unlike MMP9/TIMP1 ratio: Multivariate model
Authors: ME Niño, SE Serrano, DC Niño, DM McCosham, ME Cardenas, VP Villareal, M Lopez, A Pazin-Filh, FA Jaimes, F Cunha, R Schulz, D Torres-Due
PLoS ONE, 2017-02-13;12(2):e0171191.
Species: Human
Sample Types: Serum
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Role of serum VEGFA, TIMP2, MMP2 and MMP9 in monitoring response to adjuvant radiochemotherapy in patients with primary cervical cancer--results of a companion protocol of the randomized NOGGO-AGO phase III clinical trial.
Authors: Braicu E, Gasimli K, Richter R, Nassir M, Kummel S, Blohmer J, Yalcinkaya I, Chekerov R, Ignat I, Ionescu A, Mentze M, Fotopoulou C, Pop C, Lichtenegger W, Sehouli J
Anticancer Res, 2014-01-01;34(1):385-91.
Species: Human
Sample Types: Serum
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Role of serum concentration of VEGFR1 and TIMP2 on clinical outcome in primary cervical cancer: results of a companion protocol of the randomized, NOGGO-AGO phase III adjuvant trial of simultaneous cisplatin-based radiochemotherapy vs. carboplatin and paclitaxel containing sequential radiotherapy.
Authors: Braicu E, Fotopoulou C, Chekerov R, Richter R, Blohmer J, Kummel S, Stamatian F, Yalcinkaya I, Mentze M, Lichtenegger W, Sehouli J
Cytokine, 2013-02-14;61(3):755-8.
Species: Human
Sample Types: Serum
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Activation of matrix metalloproteinases following anti-Abeta immunotherapy; implications for microhemorrhage occurrence.
Authors: Wilcock DM, Morgan D, Gordon MN, Taylor TL, Ridnour LA, Wink DA, Colton CA
J Neuroinflammation, 2011-09-09;8(0):115.
Species: Mouse
Sample Types: Tissue Homogenates
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Increased levels of leukocyte-derived MMP-9 in patients with stable angina pectoris.
Authors: Jonasson S, Lundberg A, Kalvegren H, Bergstrom I, Szymanowski A, Jonasson L
PLoS ONE, 2011-04-29;6(4):e19340.
Species: Human
Sample Types: Serum
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Primary human acute myelogenous leukemia cells release matrix metalloproteases and their inhibitors: release profile and pharmacological modulation.
Authors: Reikvam H, Hatfield KJ, Oyan AM, Kalland KH, Kittang AO, Bruserud O
Eur. J. Haematol., 2009-11-17;84(3):239-51.
Species: Human
Sample Types: Cell Culture Supernates
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Abnormalities in metalloproteinase pathways and IGF-I axis: a link between birth weight, hypertension, and vascular damage in childhood.
Authors: Sesso R, Franco MC
Am. J. Hypertens., 2009-11-05;23(1):6-11.
Species: Human
Sample Types: Plasma
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Salbutamol up-regulates matrix metalloproteinase-9 in the alveolar space in the acute respiratory distress syndrome.
Authors: O'Kane CM, McKeown SW, Perkins GD, Bassford CR, Gao F, Thickett DR, McAuley DF
Crit. Care Med., 2009-07-01;37(7):2242-9.
Species: Human
Sample Types: BALF
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Immunomodulation by chronobiologically-based glucocorticoids treatment for multiple sclerosis relapses.
Authors: Glass-Marmor L, Paperna T, Galboiz Y, Miller A
J. Neuroimmunol., 2009-03-28;210(1):124-7.
Species: Human
Sample Types: Serum
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Imbalance of tissue inhibitors of metalloproteinases (TIMP) - 1 and - 4 serum levels, in patients with inflammatory bowel disease.
Authors: Kapsoritakis AN, Kapsoritaki AI, Davidi IP, Lotis VD, Manolakis AC, Mylonis PI, Theodoridou AT, Germenis AE, Potamianos SP
BMC Gastroenterol, 2008-11-26;8(1):55.
Species: Human
Sample Types: Serum
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MMP expression and abnormal lung permeability are important determinants of outcome in IPF.
Authors: McKeown S, Richter AG, O'Kane C, O'Kane C, McAuley DF, Thickett DR
Eur. Respir. J., 2008-10-01;33(1):77-84.
Species: Human
Sample Types: BALF
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Impact of acute exposure to tobacco smoke on gelatinases in the bronchoalveolar space.
Authors: Glader P, Eldh B, Bozinovski S, Andelid K, Sjostrand M, Malmhall C, Anderson GP, Riise GC, Qvarfordt I, Linden A
Eur. Respir. J., 2008-04-16;32(3):644-50.
Species: Human
Sample Types: BALF
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Is metalloproteinase-7 specific for idiopathic pulmonary fibrosis?
Authors: Huh JW, Kim DS, Oh YM, Shim TS, Lim CM, Lee SD, Koh Y, Kim WS, Kim WD, Kim KR
Chest, 2007-12-10;133(5):1101-6.
Species: Human
Sample Types: BALF
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Actinobacillus actinomycetemcomitans lipopolysaccharide regulates matrix metalloproteinase, tissue inhibitors of matrix metalloproteinase, and plasminogen activator production by human gingival fibroblasts: a potential role in connective tissue destruction.
Authors: Bodet C, Andrian E, Tanabe S, Grenier D
J. Cell. Physiol., 2007-07-01;212(1):189-94.
Species: Human
Sample Types: Cell Culture Supernates
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Matrix metalloproteinase-2 dysregulation in type 1 diabetes.
Authors: Thrailkill KM, Bunn RC, Moreau CS, Cockrell GE, Simpson PM, Coleman HN, Frindik JP, Kemp SF, Fowlkes JL
Diabetes Care, 2007-06-11;30(9):2321-6.
Species: Human
Sample Types: Plasma
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Plasma MMP-2 and MMP-9 and their inhibitors TIMP-1 and TIMP-2 during human orthotopic liver transplantation. The effect of aprotinin and the relation to ischemia/reperfusion injury.
Authors: Kuyvenhoven JP, Molenaar IQ, Verspaget HW, Veldman MG, Palareti G, Legnani C, Moolenburgh SE, Terpstra OT, Lamers CB, van Hoek B, Porte RJ
Thromb. Haemost., 2004-03-01;91(3):506-13.
Species: Human
Sample Types: Plasma
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Matrix metalloproteinases and tissue inhibitors of metalloproteinases in sera and tissue of patients with Dupuytren's disease.
Authors: Ulrich D, Hrynyschyn K, Pallua N
Plast. Reconstr. Surg., 2003-10-01;112(5):1279-86.
Species: Human
Sample Types: Serum
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