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Human TLR2 Antibody

Catalog #: MAB2616
25 Citations 3 Reviews Datasheet / COA / SDS
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MAB2616
MAB2616-SP
IL‑8 Secretion Induced by Pam3CSK4and Neutralization by Human TLR2 Antibody.
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Product Details
Citations (25)
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Supplemental Products
Reviews (3)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human TLR2 Antibody Summary

Species Reactivity
Human
Specificity
Stains human TLR2 transfectants but not irrelevant transfectants.
Source
Monoclonal Mouse IgG2B Clone # 383936
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
NS0 mouse myeloma cell line transfected with human TLR2
Accession # O60603
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration
Sample
Flow Cytometry
2.5 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Neutralization
Measured by its ability to neutralize Pam3CSK4-induced IL‑8 secretion in the HEK293 human embryonic kidney cell line transfected with human TLR2. The Neutralization Dose (ND50) is typically 0.03-0.15 µg/mL in the presence of 0.5 µg/mL The synthetic tripalmitoylated lipopeptide Pam3CSK4.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization IL‑8 Secretion Induced by Pam<SUB>3</SUB>CSK<SUB>4</SUB>and Neutralization by Human TLR2 Antibody. View Larger

IL‑8 Secretion Induced by Pam3CSK4and Neutralization by Human TLR2 Antibody. The synthetic tripalmitoylated lipopeptide Pam3CSK4stimulates IL-8 secretion in the HEK293 human embryonic kidney cell line transfected with human TLR2, in a dose-dependent manner (orange line), as measured by the Human CXCL8/IL-8 Quantikine ELISA Kit (Catalog # D8000C). IL-8 secretion elicited by Pam3CSK4(0.5 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human TLR2 Monoclonal Antibody (Catalog # MAB2616). The ND50 is typically 0.03-0.15 µg/mL.

Flow Cytometry Detection of TLR2 antibody in Human Monocytes antibody by Flow Cytometry. View Larger

Detection of TLR2 in Human Monocytes by Flow Cytometry. Human whole blood monocytes were stained with Mouse Anti-Human TLR2 Monoclonal Antibody (Catalog # MAB2616, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0102B).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TLR2

Human toll-like receptor (TLR) family includes ten members that activate the innate immune response via an ability to recognize molecular structures found in a variety of microbial pathogens (1‑3). All TLR family members are type I transmembrane proteins with a large number of extracellular leucine-rich repeats (LRRs) and a cytoplasmic Toll/IL-1 receptor (TIR) domain. Human TLR2 is synthesized as a 784 amino acid (aa) precursor (2) that contains a signal sequence (aa 1-18), an extracellular domain (aa 19-588) with approximately 20 LRRs, a transmembrane segment (aa 589-609), and a cytoplasmic TIR domain (aa 610-784). The receptor is expressed on a number of cell types including monocytes, dendritic cells, neutrophils, B cells endothelial cells, and hepatocytes (1, 2, 4). TLR2 functions as part of a heterodimeric complex with either TLR1 or TLR6, and possibly other co-receptors (1). These complexes recognize lipoproteins and glycolipids from gram-positive and gram-negative bacteria as well as mycoplasma and yeast. TLR2/TLR1 heterodimers bind triacylated lipopeptides, while the TLR2/TLR6 heterodimer preferentially recognizes diacylated lipopeptides (5). Upon ligand recognition, TLR2 delivers an activating signal via the associated adapter molecules, MyD88 and TIRAP (1, 6). TLR2 signaling results in dendritic cell maturation characterized by increased surface expression of class II MHC and the T cell costimulators, CD80 and CD86 (1, 2). Activation via TLR2 also results in production of a number of pro-inflammatory cytokines including TNF-alpha, IL-2, IL-6, IL-12, and MIP-2 (1-3).

References
  1. Wetzler, L. (2003) Vaccine 21:S2/55.
  2. Kirschning, C. and R. Schumann (2002) Curr. Top. microbiol. Immunol. 270:121.
  3. Netea, M. et al. (2004) J. Leukoc. Biol. 75:749.
  4. Flo, T. et al. (2001) J. Leukoc. Biol. 69:474.
  5. Akira, S. (2003) Curr. Opin. Immunol. 15:5.
  6. Yamamoto, M. et al. (2002) Nature 420:324.
Long Name
Toll-like Receptor 2
Entrez Gene IDs
7097 (Human); 24088 (Mouse); 310553 (Rat)
Alternate Names
CD282 antigen; CD282; TIL4CD282; TLR2; toll/interleukin 1 receptor-like 4; Toll/interleukin-1 receptor-like protein 4; toll-like receptor 2

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Citations for Human TLR2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

25 Citations: Showing 1 - 10
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  1. CLEC5A and TLR2 are critical in SARS-CoV-2-induced NET formation and lung inflammation
    Authors: Sung PS, Yang SP, Peng YC et al.
    Journal of biomedical science
  2. HCV inhibits M2a, M2b and M2c macrophage polarization via HCV core protein engagement with Toll‑like receptor 2
    Authors: Shixing Zhao, Meng Si, Xianpei Deng, Dengqin Wang, Lingbin Kong, Qianqian Zhang
    Experimental and Therapeutic Medicine
  3. Role of TLR4 Receptor Complex in the Regulation of the Innate Immune Response by Fibronectin
    Authors: M Zheng, A Ambesi, P J McKeown-
    Cells, 2020-01-15;9(1):.
    Applications: Neutralization
  4. The innate immune sensor Toll-like receptor 2 controls the senescence-associated secretory phenotype
    Authors: Priya Hari, Fraser R. Millar, Nuria Tarrats, Jodie Birch, Andrea Quintanilla, Curtis J. Rink et al.
    Science Advances
  5. Hepatitis B Virus–Induced Imbalance of Inflammatory and Antiviral Signaling by Differential Phosphorylation of STAT1 in Human Monocytes
    Authors: Hongxiao Song, Guangyun Tan, Yang Yang, An Cui, Haijun Li, Tianyang Li et al.
    The Journal of Immunology
  6. Pro-Inflammatory Responses in Human Bronchial Epithelial Cells Induced by Spores and Hyphal Fragments of Common Damp Indoor Molds
    Authors: Elisabeth Øya, Rune Becher, Leni Ekeren, Anani K.J. Afanou, Johan Øvrevik, Jørn A. Holme
    International Journal of Environmental Research and Public Health
  7. CLEC5A is a critical receptor in innate immunity against Listeria infection
    Authors: ST Chen, FJ Li, TY Hsu, SM Liang, YC Yeh, WY Liao, TY Chou, NJ Chen, M Hsiao, WB Yang, SL Hsieh
    Nat Commun, 2017-08-21;8(1):299.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  8. Dendritic Cell Sensing of Hydrophobic Di- and Triacylated Lipopeptides Self-Assembled within Synthetic Virus-like Particles
    Authors: R Sharma, A Ghasparian, JA Robinson, KC McCullough
    J. Immunol., 2017-06-19;0(0):.
    Species: Porcine
    Sample Types: Whole Cells
    Applications: Functional Assay
  9. Specific pathways mediating inflammasome activation by Candida parapsilosis
    Authors: A Tóth, E Zajta, K Csonka, C Vágvölgyi, MG Netea, A Gácser
    Sci Rep, 2017-02-22;7(0):43129.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  10. Kluyveromyces marxianus and Saccharomyces boulardii Induce Distinct Levels of Dendritic Cell Cytokine Secretion and Significantly Different T Cell Responses In Vitro
    PLoS ONE, 2016-11-29;11(11):e0167410.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  11. A Model to Explain How the Bacille Calmette Gu�rin (BCG) Vaccine Drives Interleukin-12 Production in Neonates
    PLoS ONE, 2016-08-29;11(8):e0162148.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  12. Vaccine-Mediated Activation of Human TLR4 Is Affected by Modulation of Culture Conditions during Whole-Cell Pertussis Vaccine Preparation
    PLoS ONE, 2016-08-22;11(8):e0161428.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  13. TLR2 Activation Limits Rhinovirus-Stimulated CXCL-10 by Attenuating IRAK-1-Dependent IL-33 Receptor Signaling in Human Bronchial Epithelial Cells
    J Immunol, 2016-08-08;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  14. Porphyromonas gingivalis Outer Membrane Vesicles Induce Selective Tumor Necrosis Factor Tolerance in a Toll-Like Receptor 4- and mTOR-Dependent Manner
    Authors: Tobias Waller, Laura Kesper, Josefine Hirschfeld, Henrik Dommisch, Johanna Kölpin, Johannes Oldenburg et al.
    Infection and Immunity
  15. HMGB1 induces angiogenesis in rheumatoid arthritis via HIF-1alpha activation.
    Authors: Park S, Lee S, Kim H, Lee W, Hong K, Kim C
    Eur J Immunol, 2015-01-15;45(4):1216-27.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  16. Necrotic cell-derived high mobility group box 1 attracts antigen-presenting cells but inhibits hepatocyte growth factor-mediated tropism of mesenchymal stem cells for apoptotic cell death.
    Authors: Vogel S, Borger V, Peters C, Forster M, Liebfried P, Metzger K, Meisel R, Daubener W, Trapp T, Fischer J, Gawaz M, Sorg R
    Cell Death Differ, 2015-01-09;22(7):1219-30.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  17. Bordetella pertussis naturally occurring isolates with altered lipooligosaccharide structure fail to fully mature human dendritic cells.
    Authors: Brummelman J, Veerman R, Hamstra H, Deuss A, Schuijt T, Sloots A, Kuipers B, van Els C, van der Ley P, Mooi F, Han W, Pinelli E
    Infect Immun, 2014-10-27;83(1):227-38.
    Species: Human
    Sample Types: Whole Cells
    Applications: Blocking
  18. Activated platelets interfere with recruitment of mesenchymal stem cells to apoptotic cardiac cells via high mobility group box 1/Toll-like receptor 4-mediated down-regulation of hepatocyte growth factor receptor MET.
    Authors: Vogel S, Chatterjee M, Metzger K, Borst O, Geisler T, Seizer P, Muller I, Mack A, Schumann S, Buhring H, Lang F, Sorg R, Langer H, Gawaz M
    J Biol Chem, 2014-02-24;289(16):11068-82.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry, Neutralization
  19. Mycobacteria bypass mucosal NF-kB signalling to induce an epithelial anti-inflammatory IL-22 and IL-10 response.
    Authors: Lutay N, Hakansson G, Alaridah N, Hallgren O, Westergren-Thorsson G, Godaly G
    PLoS ONE, 2014-01-28;9(1):e86466.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  20. Tumor-produced versican V1 enhances hCAP18/LL-37 expression in macrophages through activation of TLR2 and vitamin D3 signaling to promote ovarian cancer progression in vitro.
    Authors: Li D, Wang X, Wu J, Quan W, Ma L, Yang F, Wu K, Wan H
    PLoS ONE, 2013-02-12;8(2):e56616.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  21. Mycobacterium bovis bacilli Calmette-Guerin regulates leukocyte recruitment by modulating alveolar inflammatory responses.
    Authors: Andersson M, Lutay N, Hallgren O, Westergren-Thorsson G, Svensson M, Godaly G
    Innate Immun, 2011-11-04;18(3):531-40.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: Flow Cytometry, ICC, Neutralization, Western Blot
  22. Isotretinoin increases skin-surface levels of neutrophil gelatinase-associated lipocalin in patients treated for severe acne.
    Authors: Lumsden KR, Nelson AM, Dispenza MC, Gilliland KL, Cong Z, Zaenglein AL, Thiboutot DM
    Br. J. Dermatol., 2011-08-01;165(2):302-10.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  23. Soluble forms of Toll-like receptor 4 are present in human saliva and modulate tumour necrosis factor-alpha secretion by macrophage-like cells.
    Authors: Zunt SL, Burton LV, Goldblatt LI, Dobbins EE, Srinivasan M
    Clin. Exp. Immunol., 2009-03-09;156(2):285-93.
    Species: Human
    Sample Types: Saliva
    Applications: ELISA Development
  24. The human CD5L/AIM-CD36 axis: A novel autophagy inducer in macrophages that modulates inflammatory responses
    Authors: Sanjurjo L, Amezaga N, Aran G et al.
    Autophagy.
  25. Platelets mediate inflammatory monocyte activation by SARS-CoV-2 Spike protein
    Authors: Li T, Yang Y, Li Y et al.
    The Journal of clinical investigation

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Reviews for Human TLR2 Antibody

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Human TLR2 Antibody
By Anonymous on 12/13/2019
Application: MiAr Sample Tested: EDTA Plasma Species: Human

Antibody was printed on custom arrays and incubated with fluorescently labeled human EDTA plasma

Microarray Human TLR2 Antibody MAB2616
Human TLR2 Antibody
By Anonymous on 03/11/2019
Application: Microarrays Sample Tested: EDTA Plasma Species: Human
Human TLR2 Antibody
By Anonymous on 04/26/2016
Application: Flow Sample Tested: KF6 human keratinocytes,THP-1 human acute monocytic leukemia cell line Species: Human
Flow Cytometry Human TLR2 Antibody MAB2616