Human Tollip Antibody

Catalog #: AF4678 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF4678
AF4678-SP

Detection of Human Tollip by Western Blot.

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Human Tollip Antibody Summary

Species Reactivity

Human

Specificity

Detects endogenous human Tollip in Western blots.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human Tollip
Met1-Pro274
Accession # Q9H0E2

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human Tollip by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and LNCaP human prostate cancer cell line. PVDF membrane was probed with 1 µg/mL of Human Tollip Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4678) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Tollip at approximately 30 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 4.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Tollip

Tollip (Toll-interacting protein) is a 30 kDa, cytoplasmic protein that is involved in IL-1 and Toll receptor signaling. It is classified as an adaptor protein that performs multiple functions. It binds both IRAK and Toll receptors, inhibiting constitutive IL-1 and Toll receptor signaling. It also binds to ubiquinated IL-1 RI, directing it to the endosomal degradation compartment. Human Tollip is 274 amino acids (aa) in length. It contains a C2 Ca⁺⁺-binding region (aa 54‑154) and a CUE domain (aa 229‑270) that binds ubiquitin. One potential splice form exists that shows a deletion of aa’s 6‑33. Human Tollip is 92% identical to mouse and canine Tollip.

Long Name

Toll Interacting Protein

Entrez Gene IDs

54472 (Human); 54473 (Mouse); 361677 (Rat)

Alternate Names

adapter protein; FLJ33531; IL-1RAcPIP; toll interacting protein; toll-interacting protein; Tollip