Human TSPAN1 Antibody

Catalog #: MAB9186 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB9186-100
MAB9186-SP

Detection of TSPAN1 in HT‑29 Human Cell Line by Flow Cytometry.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human TSPAN1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human TSPAN1 in direct ELISAs.

Source

Monoclonal Mouse IgG₃ Clone # 819202

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human TSPAN1
Met1-Gln241
Accession # O60635

Formulation

Lyophilized from a 0.2 μm filtered solution in TBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of TSPAN1 in HT‑29 Human Cell Line by Flow Cytometry. HT-29 human colon adenocarcinoma cell line was stained with Mouse Anti-Human TSPAN1 Monoclonal Antibody (Catalog # MAB9186, filled histogram) or isotype control antibody (Catalog # MAB007, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry

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Detection of TSPAN1 in HEK293 Human Cell Line Transfected with Human TSPAN1 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with either (A) human TSPAN1 or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human TSPAN1 Monoclonal Antibody (Catalog # MAB9186) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB007). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TSPAN1

Tetraspanins (TSPAN family) are small integral proteins that contain four transmembrane domains with two different and well characterized extracellular domains. Tetraspanins are found on the plasma membrane and within various intracellular organelles and granules in nearly all cell and tissue types. They interact with one another and with other transmembrane molecules, including integrins, generating a ‘web' of interactions believed to control cell and membrane trafficking and compartmentalization. Tetraspanin-1 (TSPAN1), a 241 amino acid protein, interacts with the thiamine transporter-1 (THTR-1, SLC19A2) in human intestinal epithelial cells, linking TSPAN1 to the regulation of thiamine uptake. TSPAN1 has been found to be expressed in gastric, ovarian, colon, and other carcinomas.

Long Name

Tetraspanin 1

Entrez Gene IDs

10103 (Human); 66805 (Mouse); 298436 (Rat)

Alternate Names

NET1; NET-1; tetraspan 1; Tetraspan NET-1; tetraspanin 1; Tetraspanin TM4-C; tetraspanin-1; TM4C; TM4SF; TSPAN1; TSPAN-1