Human u-Plasminogen Activator (uPA)/Urokinase Biotinylated Antibody

Catalog #: BAF1310 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
BAF1310

All u-Plasminogen Activator (uPA)/Urokinase Antibodies

Product Details

Supplemental Products

Reviews

Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human u-Plasminogen Activator (uPA)/Urokinase Biotinylated Antibody Summary

Species Reactivity

Human

Specificity

Detects human u-Plasminogen Activator in ELISAs and Western blots. In sandwich immunoassays, less than 0.2% cross‑reactivity with rhThrombin, rhPlasminogen and htPA is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human uPA (R&D Systems, Catalog # 1310-SE)
Ser21-Leu432
Accession # P00749

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human u-Plasminogen Activator (uPA)/Urokinase (Catalog # 1310-SE)

Human u-Plasminogen Activator/Urokinase Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Detection (Matched Antibody Pair)

0.1-0.4 µg/mL

Use in combination with:

Capture Reagent: Human u-Plasminogen Activator (uPA)/
Urokinase Catalytic Domain Antibody (Catalog # MAB1310)

Standard: Recombinant Human u-Plasminogen Activator/Urokinase, CF (Catalog # 1310-SE)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: u-Plasminogen Activator (uPA)/Urokinase

uPA is a serine protease with an extremely limited substrate specificity, cleaving the sequence Cys-Pro-Gly-Arg560-Val561-Val-Gly-Gly-Cys in plasminogen to form plasmin (1). uPA is a potent marker of invasion and metastasis in a variety of human cancers associated with breast, stomach, colon, bladder, ovary, brain and endometrium (2). For example, the combination (both low vs. either or both high) of uPA and its inhibitor, plasminogen activator inhibitor-1 (PAI-1), outperforms the single factors as well as other traditional prognostic factors with regard to risk group assessment for breast cancer, particularly in node-negative breast cancer (3). The human uPA is initially synthesized as 431 amino acid precursor with a N-terminal signal peptide (20 residues) (4 - 6). The single chain molecule is processed into a disulfide-linked two-chain molecule. The B chain starting at Ile179 corresponds to the catalytic domain. Two forms of the A chain exist, one starting at Ser21 (the long form) and the other at Lys156 (the short form). The resulting two-chain forms have different molecular weights (MW). The B chain is common for both forms whereas the long and short A chains are unique to the high and low MW forms, respectively. The long A chain contains an EGF-like domain, which is responsible for binding of the uPA receptor (uPAR).

References

Ellis, V. (2004) in Handbook of Proteolytic Enzymes. Barrett, A.J. et al. eds., Academic Press, San Diego, pp.1677.
Duffy, M.J. (2002) Biochem. Soc. Trans. 30:207.
Harbeck, N. et al. (2002) Clin. Breast Cancer 3:196.
Riccio, A. et al. (1985) Nucleic Acids Res. 13:2785.
Nagai, M. et al. (1985) Gene 36:183.
Jacobs, P. et al. (1985) DNA 4:139.

Long Name

Urokinase-type Plasminogen Activator

Entrez Gene IDs

5328 (Human); 18792 (Mouse); 102135886 (Cynomolgus Monkey)

Alternate Names

ATF; EC 3.4.21; EC 3.4.21.73; plasminogen activator, urokinase; PLAU; uPA; u-PA; uPlasminogen Activator; u-Plasminogen Activator; urinary; Urokinase; urokinase-type plasminogen activator