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Human VEGFR1/Flt-1 Antibody

Catalog #: MAB321
14 Citations 2 Reviews Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB321-100
MAB321-SP
Detection of VEGFR1/Flt‑1 in HUVEC Human Cells by Flow Cytometry.
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Product Details
Citations (14)
FAQs
Supplemental Products
Reviews (2)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human VEGFR1/Flt-1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human VEGFR1/Flt-1 in direct ELISAs and Western blots. In Western blots, this antibody does not cross-react with recombinant mouse VEGFR1, recombinant human (rh) VEGFR2, rhVEGFR3, or rhVEGFR4.
Source
Monoclonal Mouse IgG1 Clone # 49560
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human VEGFR1/Flt-1
Ser27-His687
Accession # NP_001153392
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human VEGFR1/Flt-1 Fc Chimera (Catalog # 321-FL)
under non-reducing conditions only
Flow Cytometry
2.5 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of VEGFR1/Flt-1 antibody in HUVEC Human Cells antibody by Flow Cytometry. View Larger

Detection of VEGFR1/Flt‑1 in HUVEC Human Cells by Flow Cytometry. HUVEC human umbilical vein endothelial cells was stained with Mouse Anti-Human VEGFR1/Flt-1 Monoclonal Antibody (Catalog # MAB321, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: VEGFR1/Flt-1

Vascular Endothelial Growth Factor Receptor 1 (VEGFR1) is a receptor tyrosine kinase that is expressed primarily on endothelial cells and plays a role in vasculogenesis and angiogenesis. A soluble variant of VEGFR1 was also reported to bind VEGF and PlGF with high affinity and function as a potent VEGF antagonist.

Long Name
Vascular Endothelial Growth Factor Receptor 1
Entrez Gene IDs
2321 (Human); 14254 (Mouse)
Alternate Names
EC 2.7.10; EC 2.7.10.1; FLT; FLT1; Flt-1; Fms-like tyrosine kinase 1; fms-related tyrosine kinase 1 (vascular endothelial growth factor/vascularpermeability factor receptor); FRT; Tyrosine-protein kinase FRT; Tyrosine-protein kinase receptor FLT; vascular endothelial growth factor receptor 1; Vascular permeability factor receptor; VEGF R1; VEGFR1; VEGFR-1

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Citations for Human VEGFR1/Flt-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

14 Citations: Showing 1 - 10
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  1. Atypical chemokine receptor ACKR3/CXCR7 controls postnatal vasculogenesis and arterial specification by mesenchymal stem cells via Notch signaling
    Authors: ST Wei, YC Huang, ML Hsieh, YJ Lin, WC Shyu, HC Chen, CH Hsieh
    Cell Death Dis, 2020-05-04;11(5):307.
  2. The Soluble Fms-like Tyrosine Kinase-1 Contributes to Structural and Functional Changes in Endothelial Cells in Chronic Kidney Disease
    Authors: A Schulz, CC Drost, B Hesse, K Beul, M Brand, GS Di Marco
    International Journal of Molecular Sciences, 2022-12-16;23(24):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture
  3. Synergic effects of VEGF-A and SDF-1 on the angiogenic properties of endothelial progenitor cells
    Authors: Gabriela Odent Grig
    J Tissue Eng Regen Med, 2016-12-12;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Placental-Specific sFLT-1 e15a Protein Is Increased in Preeclampsia, Antagonizes Vascular Endothelial Growth Factor Signaling, and Has Antiangiogenic Activity
    Authors: Kirsten R. Palmer, Tu’uhevaha J. Kaitu’u-Lino, Roxanne Hastie, Natalie J. Hannan, Louie Ye, Natalie Binder et al.
    Hypertension
  5. Vasoreparative dysfunction of CD34+ cells in diabetic individuals involves hypoxic desensitization and impaired autocrine/paracrine mechanisms.
    Authors: Jarajapu Y, Hazra S, Segal M, Li Calzi S, Jadhao C, Qian K, Mitter S, Raizada M, Boulton M, Grant M
    PLoS ONE, 2014-04-08;9(4):e93965.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. Tumour hypoxia promotes tolerance and angiogenesis via CCL28 and T(reg) cells.
    Authors: Facciabene A, Peng X, Hagemann IS, Balint K, Barchetti A, Wang LP, Gimotty PA, Gilks CB, Lal P, Zhang L, Coukos G
    Nature, 2011-07-13;475(7355):226-30.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  7. Expression of growth factor receptors and targeting of EGFR in cholangiocarcinoma cell lines
    Authors: Ling Xu, Martin Hausmann, Wolfgang Dietmaier, Silvia Kellermeier, Theresa Pesch, Manuela Stieber-Gunckel et al.
    BMC Cancer
  8. Placental growth factor neutralising antibodies give limited anti-angiogenic effects in an in vitro organotypic angiogenesis model.
    Authors: Brave SR, Eberlein C, Shibuya M, Wedge SR, Barry ST
    Angiogenesis, 2010-10-16;13(4):337-47.
    Species: Human
    Sample Types: Cell Lysates, Whole Tissue
    Applications: ELISA Development, IHC
  9. Down-regulation of vascular endothelial growth factor receptor 2 is a major molecular determinant of proteasome inhibitor-mediated antiangiogenic action in endothelial cells.
    Authors: Meissner M, Reichenbach G, Stein M, Hrgovic I, Kaufmann R, Gille J
    Cancer Res., 2009-02-17;69(5):1976-84.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  10. Resistance of acute myeloid leukemic cells to the triterpenoid CDDO-Imidazolide is associated with low caspase-8 and FADD levels.
    Authors: Riccioni R, Senese M, Diverio D, Riti V, Mariani G, Boe A, LoCoco F, Foa R, Peschle C, Sporn M, Testa U
    Leuk. Res., 2008-03-04;32(8):1244-58.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  11. Placental growth factor down-regulates type 1 T helper immune response by modulating the function of dendritic cells.
    Authors: Lin YL, Liang YC, Chiang BL
    J. Leukoc. Biol., 2007-08-30;82(6):1473-80.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  12. Expression of Tie-2 and other receptors for endothelial growth factors in acute myeloid leukemias is associated with monocytic features of leukemic blasts.
    Authors: Riccioni R, Diverio D, Mariani G, Buffolino S, Riti V, Saulle E, Petrucci E, Cedrone M, Lo-Coco F, Foa R, Peschle C, Testa U
    Stem Cells, 2007-04-19;25(8):1862-71.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  13. Dissecting the role of endothelial SURVIVIN DeltaEx3 in angiogenesis.
    Authors: Caldas H, Fangusaro JR, Boue DR, Holloway MP, Altura RA
    Blood, 2006-10-12;109(4):1479-89.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  14. Plasma angiopoietin-1, angiopoietin-2 and Tie-2 in breast and prostate cancer: a comparison with VEGF and Flt-1.
    Authors: Caine GJ, Blann AD, Stonelake PS, Ryan P, Lip GY
    Eur. J. Clin. Invest., 2003-10-01;33(10):883-90.
    Species: Human
    Sample Types: Plasma
    Applications: ELISA Development

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Reviews for Human VEGFR1/Flt-1 Antibody

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Human VEGFR1/Flt-1 Antibody
By Anonymous on 07/13/2024
Application: Flow Sample Tested: retinal cells Species: Human
Flow Cytometry Human VEGFR1/Flt-1 Antibody MAB321
Human VEGFR1/Flt-1 Antibody
By Anonymous on 09/10/2021
Application: WB Sample Tested: endothelial cells Species: Human
Western Blot Human VEGFR1/Flt-1 Antibody MAB321