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Human VEGFR1/Flt-1 PE-conjugated Antibody

Catalog #: FAB321P
22 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
FAB321P
Detection of VEGF R1/Flt‑1 in HUVEC Human Cells by Flow Cytometry.
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Citations (22)
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Human VEGFR1/Flt-1 PE-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human VEGF R1/Flt-1 in direct ELISAs and Western blots. In Western blots, this antibody does not cross-react with recombinant mouse VEGF R1, recombinant human (rh) VEGF R2, rhVEGF R3, or rhVEGF R4.
Source
Monoclonal Mouse IgG1 Clone # 49560
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human VEGF R1/Flt-1
Ser27-His687
Accession # NP_001153392
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of VEGF R1/Flt-1 antibody in HUVEC Human Cells antibody by Flow Cytometry. View Larger

Detection of VEGF R1/Flt‑1 in HUVEC Human Cells by Flow Cytometry. HUVEC human umbilical vein endothelial cells were stained with Mouse Anti-Human VEGF R1/Flt-1 PE-conjugated Monoclonal Antibody (Catalog # FAB321P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: VEGFR1/Flt-1

Vascular Endothelial Growth Factor Receptor 1 (VEGF R1) is a receptor tyrosine kinase that is expressed primarily on endothelial cells and plays a role in vasculogenesis and angiogenesis. A soluble variant of VEGF R1 was also reported to bind VEGF and PlGF with high affinity and function as a potent VEGF antagonist.

Long Name
Vascular Endothelial Growth Factor Receptor 1
Entrez Gene IDs
2321 (Human); 14254 (Mouse)
Alternate Names
EC 2.7.10; EC 2.7.10.1; FLT; FLT1; Flt-1; Fms-like tyrosine kinase 1; fms-related tyrosine kinase 1 (vascular endothelial growth factor/vascularpermeability factor receptor); FRT; Tyrosine-protein kinase FRT; Tyrosine-protein kinase receptor FLT; vascular endothelial growth factor receptor 1; Vascular permeability factor receptor; VEGF R1; VEGFR1; VEGFR-1

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Citations for Human VEGFR1/Flt-1 PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

22 Citations: Showing 1 - 10
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  1. VEGFR1 primes a unique cohort of dental pulp stem cells for vasculogenic differentiation
    Authors: MT Bergamo, Department of Cariology, Restorative Sciences and Endodontics, University of Michigan School of Dentistry, 1011 N. University Rm. G049, Ann Arbor, MI 48109-1078, USA, Z Zhang, TM Oliveira, JE Nör
    European Cells and Materials
  2. A quantitative method for screening and identifying molecular targets for nanomedicine
    Authors: P Guo, J Yang, DR Bielenberg, D Dillon, D Zurakowski, MA Moses, DT Auguste
    J Control Release, 2017-03-22;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. The distribution and potential prognostic value of SMAD protein expression in chronic lymphocytic leukemia
    Authors: M Witkowska, A Majchrzak, B Cebula-Obr, E Wawrzyniak, T Robak, P Smolewski
    Tumour Biol, 2017-03-01;39(3):1010428317694.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. BRAF Inhibition Stimulates Melanoma-Associated Macrophages to Drive Tumor Growth.
    Authors: Wang T, Xiao M, Ge Y, Krepler C, Belser E, Lopez-Coral A, Xu X, Zhang G, Azuma R, Liu Q, Liu R, Li L, Amaravadi R, Xu W, Karakousis G, Gangadhar T, Schuchter L, Lieu M, Khare S, Halloran M, Herlyn M, Kaufman R
    Clin Cancer Res, 2015-01-23;21(7):1652-64.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Differential sensitivity of prostate tumor derived endothelial cells to sorafenib and sunitinib.
    Authors: Fiorio Pla, Alessand, Brossa, Alessia, Bernardini, Michela, Genova, Tullio, Grolez, Guillaum, Villers, Arnaud, Leroy, Xavier, Prevarskaya, Natalia, Gkika, Dimitra, Bussolati, Benedett
    BMC Cancer, 2014-12-12;14(0):939.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. Antiangiogenic cancer drug sunitinib exhibits unexpected proangiogenic effects on endothelial cells.
    Authors: Norton, Kerri-An, Han, Zheyi, Popel, Aleksand, Pandey, Niranjan
    Onco Targets Ther, 2014-09-09;7(0):1571-82.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. Metabolic impact of anti-angiogenic agents on U87 glioma cells.
    Authors: Mesti T, Savarin P, Triba M, Le Moyec L, Ocvirk J, Banissi C, Carpentier A
    PLoS ONE, 2014-06-12;9(6):e99198.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  8. Laminin promotes vascular network formation in 3D in vitro collagen scaffolds by regulating VEGF uptake.
    Authors: Stamati K, Priestley J, Mudera V, Cheema U
    Exp Cell Res, 2014-06-05;327(1):68-77.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  9. Interaction of mesenchymal stem cells with fibroblast-like synoviocytes via cadherin-11 promotes angiogenesis by enhanced secretion of placental growth factor.
    Authors: Park, Su-Jung, Kim, Ki-Jo, Kim, Wan-Uk, Cho, Chul-Soo
    J Immunol, 2014-02-26;192(7):3003-10.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  10. Immunophenotypic characterization of human monocyte subsets: possible implications for cardiovascular disease pathophysiology.
    Authors: Shantsila E, Wrigley B, Tapp L, Apostolakis S, Montoro-Garcia S, Drayson MT, Lip GY
    J. Thromb. Haemost., 2011-05-01;9(5):1056-66.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  11. Oligo-guanosine nucleotide induces neuropilin-1 internalization in endothelial cells and inhibits angiogenesis.
    Authors: Narazaki M, Segarra M, Hou X, Tanaka T, Li X, Tosato G
    Blood, 2010-07-06;116(16):3099-107.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  12. Multiple receptor tyrosine kinases regulate HIF-1alpha and HIF-2alpha in normoxia and hypoxia in neuroblastoma: implications for antiangiogenic mechanisms of multikinase inhibitors.
    Authors: Nilsson MB, Zage PE, Zeng L, Xu L, Cascone T, Wu HK, Saigal B, Zweidler-McKay PA, Heymach JV
    Oncogene, 2010-03-08;29(20):2938-49.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  13. VEGFR2 is selectively expressed by FOXP3high CD4+ Treg.
    Authors: Suzuki H, Onishi H, Wada J, Yamasaki A, Tanaka H, Nakano K, Morisaki T, Katano M
    Eur. J. Immunol., 2010-01-01;40(1):197-203.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  14. Expression of soluble vascular endothelial growth factor receptor-1 in human monocyte-derived mature dendritic cells contributes to their antiangiogenic property.
    Authors: Kishuku M, Nishioka Y, Abe S, Kishi J, Ogino H, Aono Y, Azuma M, Kinoshita K, Batmunkh R, Rentsenhand B, Makino H, Ranjan P, Minakuchi K, Sone S
    J. Immunol., 2009-12-15;183(12):8176-85.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  15. Highly efficient and feeder-free production of subculturable vascular endothelial cells from primate embryonic stem cells.
    Authors: Saeki K, Yogiashi Y, Nakahara M, Nakamura N, Matsuyama S, Koyanagi A, Yagita H, Koyanagi M, Kondo Y, Yuo A
    J. Cell. Physiol., 2008-10-01;217(1):261-80.
    Species: Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  16. Tumor necrosis factor-alpha and endothelial cells modulate Notch signaling in the bone marrow microenvironment during inflammation.
    Authors: Fernandez L, Rodriguez S, Huang H, Chora A, Fernandes J, Mumaw C, Cruz E, Pollok K, Cristina F, Price JE, Ferkowicz MJ, Scadden DT, Clauss M, Cardoso AA, Carlesso N
    Exp. Hematol., 2008-05-01;36(5):545-558.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  17. Sulfated polysaccharides identified as inducers of neuropilin-1 internalization and functional inhibition of VEGF165 and semaphorin3A.
    Authors: Narazaki M, Segarra M, Tosato G
    Blood, 2008-02-13;111(8):4126-36.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  18. Notch alters VEGF responsiveness in human and murine endothelial cells by direct regulation of VEGFR-3 expression.
    Authors: Shawber CJ, Funahashi Y, Francisco E, Vorontchikhina M, Kitamura Y, Stowell SA, Borisenko V, Feirt N, Podgrabinska S, Shiraishi K, Chawengsaksophak K, Rossant J, Accili D, Skobe M, Kitajewski J
    J. Clin. Invest., 2007-11-01;117(11):3369-82.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  19. Vascular endothelial growth factor can signal through platelet-derived growth factor receptors.
    Authors: Ball SG, Shuttleworth CA, Kielty CM
    J. Cell Biol., 2007-04-30;177(3):489-500.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  20. Human liver sinusoidal endothelial cells induce apoptosis in activated T cells: a role in tolerance induction.
    Authors: Karrar A, Broome U, Uzunel M, Qureshi AR, Sumitran-Holgersson S
    Gut, 2006-07-13;56(2):243-52.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  21. Expansion of human SCID-repopulating cells under hypoxic conditions.
    Authors: Danet GH, Pan Y, Luongo JL, Bonnet DA, Simon MC
    J. Clin. Invest., 2003-07-01;112(1):126-35.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  22. Decrease of Pro-Angiogenic Monocytes Predicts Clinical Response to Anti-Angiogenic Treatment in Patients with Metastatic Renal Cell Carcinoma
    Authors: Stephane Oudard, Nadine Benhamouda, Bernard Escudier, Patrice Ravel, Thi Tran, Emeline Levionnois et al.
    Cells

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