Home / VEGFR2/KDR/Flk-1 / Human VEGFR2/KDR/Flk-1 Antibody

Human VEGFR2/KDR/Flk-1 Antibody

Catalog #: MAB3571
23 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB3571-SP
MAB3571-500
MAB3571-100
VEGFR2/KDR/Flk‑1 in Human Placenta.
3 Images
Product Details
Citations (23)
FAQs
Supplemental Products
Reviews
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human VEGFR2/KDR/Flk-1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human VEGFR2 in direct ELISAs and Western blots. In Western blots, this antibody does not cross-react with recombinant human (rh) VEGFR1, rhVEGFR3, or recombinant mouse VEGFR2.
Source
Monoclonal Mouse IgG1 Clone # 89115
Purification
Protein A or G purified from ascites
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human VEGFR2
Ala20-Glu764
Accession # P35968
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human VEGFR2/KDR/Flk-1 Fc Chimera (Catalog # 357-KD)
under non-reducing conditions only. It is recommended to use Goat Anti-Human VEGFR2/KDR/Flk‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF357)
Immunohistochemistry
8-25 µg/mL
See below
Immunocytochemistry
5-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry VEGFR2/KDR/Flk-1 antibody in Human Placenta by Immunohistochemistry (IHC-P). View Larger

VEGFR2/KDR/Flk‑1 in Human Placenta. VEGFR2/KDR/Flk-1 was detected in immersion fixed paraffin-embedded sections of human placenta using 25 µg/mL Human VEGFR2/KDR/Flk-1 Monoclonal Antibody (Catalog # MAB3571) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Immunohistochemistry VEGFR2/KDR/Flk-1 antibody in Human Kidney by Immunohistochemistry (IHC-P). View Larger

VEGFR2/KDR/Flk‑1 in Human Kidney. VEGFR2/KDR/Flk-1 was detected in immersion fixed paraffin-embedded sections of human kidney array using Human VEGFR2/KDR/Flk-1 Monoclonal Antibody (Catalog # MAB3571) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Immunocytochemistry VEGFR2/KDR/Flk-1 antibody in HUVECs and Human HeLa Cell Line by Immunocytochemistry (ICC). View Larger

VEGFR2/KDR/Flk‑1 in HUVECs and Human HeLa Cell Line. VEGFR2/KDR/Flk-1 was detected in immersion fixed human umbilical vein endothelial cells (HUVECs; left panel) but is not detected in Hela human cervical epithelial carcinoma cell line (right panel) using Mouse Anti-Human VEGFR2/KDR/Flk-1 Monoclonal Antibody (Catalog # MAB3571) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm of HUVECs. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Loading...
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: VEGFR2/KDR/Flk-1

VEGFR2 (KDR/Flk-1), VEGFR1 (Flt-1), and VEGFR3 (Flt-4) belong to the class III subfamily of receptor tyrosine kinases (RTKs). All three receptors contain seven immunoglobulin-like repeats in their extracellular domains and kinase insert domains in their intracellular regions. The expression of VEGFR1, 2, and 3 is almost exclusively restricted to the endothelial cells. These receptors are likely to play essential roles in vasculogenesis and angiogenesis.

VEGFR2 cDNA encodes a 1356 amino acid (aa) residue precursor protein with a 19 aa residue signal peptide. Mature VEGFR2 is composed of a 745 aa residue extracellular domain, a 25 aa residue transmembrane domain and a 567 aa residue cytoplasmic domain. In contrast to VEGF R1 which binds both PlGF and VEGF with high affinity, VEGFR2 binds VEGF but not PlGF with high affinity. The recombinant soluble VEGFR2/Fc chimera binds VEGF with high affinity and is a potent VEGF antagonist.

References
  1. Ferra, N. and R. Davis-Smyth (1997) Endocrine Reviews 18:4.
Long Name
Vascular Endothelial Growth Factor Receptor 2
Entrez Gene IDs
3791 (Human); 16542 (Mouse)
Alternate Names
CD309 antigen; CD309; EC 2.7.10; EC 2.7.10.1; Fetal liver kinase 1; fetal liver kinase-1; Flk1; Flk-1; FLK1tyrosine kinase growth factor receptor; KDR; kinase insert domain receptor (a type III receptor tyrosine kinase); Kinase insert domain receptor; KRD1; Ly73; Protein-tyrosine kinase receptor flk-1; soluble VEGFR2; vascular endothelial growth factor receptor 2; VEGF R2; VEGFR; VEGFR2; VEGFR-2

Product Datasheets

You must select a language.

x
Product Datasheet
COA
SDS

Citations for Human VEGFR2/KDR/Flk-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

23 Citations: Showing 1 - 10
Filter your results:

Filter by:

  1. Augmenting the Angiogenic Profile and Functionality of Cord Blood Endothelial Colony-Forming Cells by Indirect Priming with Bone-Marrow-Derived Mesenchymal Stromal Cells
    Authors: Bansal, A;Singh, A;Nag, TC;Sharma, D;Garg, B;Bhatla, N;Choudhury, SD;Ramakrishnan, L;
    Biomedicines
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  2. Role of endothelial colony forming cells (ECFCs) Tetrahydrobiopterin (BH4) in determining ECFCs functionality in coronary artery disease (CAD) patients
    Authors: A Sen, A Singh, A Roy, S Mohanty, N Naik, M Kalaivani, L Ramakrishn
    Scientific Reports, 2022-02-23;12(1):3076.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  3. Expression of IL-20 Receptor Subunit beta Is Linked to EAE Neuropathology and CNS Neuroinflammation
    Authors: JR Dayton, Y Yuan, LP Pacumio, BG Dorflinger, SC Yoo, MJ Olson, SI Hernández-, MM McMahon, L Cruz-Oreng
    Frontiers in Cellular Neuroscience, 2021-09-07;15(0):683687.
    Species: Rhesus Macaque
    Sample Types: Whole Tissue
    Applications: IHC
  4. Targeting QKI-7 in vivo restores endothelial cell function in diabetes
    Authors: C Yang, M Eleftheria, S Kelaini, T Morrison, MV González, R Caines, N Edwards, A Yacoub, K Edgar, A Moez, A Ivetic, A Zampetaki, L Zeng, FL Wilkinson, N Lois, AW Stitt, DJ Grieve, A Margariti
    Nat Commun, 2020-07-30;11(1):3812.
    Species: Human, Mouse
    Sample Types: Cell Lysates, Protein
    Applications: Western Blot
  5. Cucurbita argyrosperma Seed Extracts Attenuate Angiogenesis in a Corneal Chemical Burn Model
    Authors: María Fernanda Estrella-Mendoza, Francisco Jiménez-Gómez, Adolfo López-Ornelas, Rosa Martha Pérez-Gutiérrez, Javier Flores-Estrada
    Nutrients
  6. Enhanced Function of Induced Pluripotent Stem Cell-Derived Endothelial Cells Through ESM1 Signaling
    Authors: Marta Vilà-González, Sophia Kelaini, Corey Magee, Rachel Caines, David Campbell, Magdalini Eleftheriadou et al.
    Stem Cells
  7. Correlation of Vascular Endothelial Growth Factor subtypes and their receptors with melanoma progression: A next-generation Tissue Microarray (ngTMA) automated analysis
    Authors: SM Seyed Jafa, C Wiedmer, S Cazzaniga, Ž Frangež, M Shafighi, H Beltramine, B Weber, HU Simon, RE Hunger
    PLoS ONE, 2018-11-08;13(11):e0207019.
    Species: Human
    Sample Types: Tissue Microarray
    Applications: IHC-P
  8. Enhanced rapamycin delivery to hemangiomas by lipid polymer nanoparticles coupled with anti-VEGFR antibody
    Authors: H Li, Y Teng, X Xu, J Liu
    Int. J. Mol. Med., 2018-02-28;0(0):.
    Species: Human
    Sample Types: Cell Lysates, Nanoparticles, Whole Cells
    Applications: Drug Delivery, Neutralization, Western Blot
  9. Delivery of sodium morrhuate to hemangioma endothelial cells using immunoliposomes conjugated with anti-VEGFR2/KDR antibody
    Authors: X Li, X Ren, J Liang, W Ma, Z Wang, Z Yang
    Int J Nanomedicine, 2017-09-19;12(0):6963-6972.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  10. Analysis of angiogenesis related factors in glioblastoma, peritumoral tissue and their derived cancer stem cells.
    Authors: Alessio D'alessio, Gabriella Proietti, Gina Lama, Filippo Biamonte, Libero Lauriola, Umberto Moscato, Angelo Vescovi, Annunziato Mangiola, Cristiana Angelucci, Gigliola Sica
    Oncotarget, 2016-11-29;0(0):1949-2553.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  11. Endothelial progenitor cells from human fetal aorta cure diabetic foot in a rat model
    Authors: Jin-Ning Lou
    Metab. Clin. Exp., 2016-09-28;65(12):1755-1767.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry, ICC
  12. A Functional Role for VEGFR1 Expressed in Peripheral Sensory Neurons in Cancer Pain
    Authors: Deepitha Selvaraj, Vijayan Gangadharan, Christoph W. Michalski, Martina Kurejova, Sebastian Stösser, Kshitij Srivastava et al.
    Cancer Cell
  13. The effect of vascular endothelial growth factor on osteoclastogenesis in rheumatoid arthritis.
    Authors: Kim H, Kim K, Kim B, Cho M, Lee S
    PLoS ONE, 2015-04-20;10(4):e0124909.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: IHC-Fr
  14. Early VEGFR2 activation in response to flow is VEGF-dependent and mediated by MMP activity
    Authors: Nathaniel G. dela Paz, Benoît Melchior, John A. Frangos
    Biochemical and Biophysical Research Communications
  15. A novel serum-free monolayer culture for orderly hematopoietic differentiation of human pluripotent cells via mesodermal progenitors.
    Authors: Niwa A, Heike T, Umeda K
    PLoS ONE, 2011-07-27;6(7):e22261.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  16. Cathepsin B and uPAR knockdown inhibits tumor-induced angiogenesis by modulating VEGF expression in glioma
    Authors: Rama Rao Malla, Sreelatha Gopinath, Christopher S. Gondi, Kiranmai Alapati, Dzung H. Dinh, Meena Gujrati et al.
    Cancer Gene Therapy
  17. Comparison between Culture Conditions Improving Growth and Differentiation of Blood and Bone Marrow Cells Committed to the Endothelial Cell Lineage
    Authors: Claudio Muscari, Chiara Gamberini, Ilaria Basile, Francesca Bonafé, Simond Valgimigli, Ombretta Capitani et al.
    Biological Procedures Online
  18. Tumour vascularization via endothelial differentiation of glioblastoma stem-like cells.
    Authors: Ricci-Vitiani L, Pallini R, Biffoni M, Todaro M, Invernici G, Cenci T, Maira G, Parati EA, Stassi G, Larocca LM, De Maria R
    Nature, 2010-11-21;468(7325):824-8.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  19. Adenoviral expression of vascular endothelial growth factor splice variants differentially regulate bone marrow-derived mesenchymal stem cells.
    Authors: Lin H, Shabbir A, Molnar M, Yang J, Marion S, Canty JM, Lee T
    J. Cell. Physiol., 2008-08-01;216(2):458-68.
    Species: Porcine
    Sample Types: Whole Cells
    Applications: ICC
  20. Development of anti-atherosclerotic tissue-engineered blood vessel by A20-regulated endothelial progenitor cells seeding decellularized vascular matrix.
    Authors: Zhu C, Ying D, Mi J, Li L, Zeng W, Hou C, Sun J, Yuan W, Wen C, Zhang W
    Biomaterials, 2008-06-01;29(17):2628-36.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  21. Thrombopoietin enhances generation of CD34+ cells from human embryonic stem cells.
    Authors: Srivastava AS, Nedelcu E, Esmaeli-Azad B, Mishra R, Carrier E
    Stem Cells, 2007-03-22;25(6):1456-61.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  22. Expression of VEGFR-2 on HaCaT cells is regulated by VEGF and plays an active role in mediating VEGF induced effects.
    Authors: Yang XH, Man XY, Cai SQ, Yao YG, Bu ZY, Zheng M
    Biochem. Biophys. Res. Commun., 2006-08-14;349(1):31-8.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Neutralization, Western Blot
  23. Isolation of renal progenitor cells from adult human kidney.
    Authors: Bussolati B, Bruno S, Grange C, Buttiglieri S, Deregibus MC, Cantino D, Camussi G
    Am. J. Pathol., 2005-02-01;166(2):545-55.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs
Loading...

Reviews for Human VEGFR2/KDR/Flk-1 Antibody

There are currently no reviews for this product. Be the first to review Human VEGFR2/KDR/Flk-1 Antibody and earn rewards!

Have you used Human VEGFR2/KDR/Flk-1 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review