Human WT1 Antibody

Detection of Human WT1 by Western Blot. Western blot shows lysates of K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human WT1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5729) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for WT1 at approximately 54 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Detection of WT1 in HL‑60 Human Cell Line by Flow Cytometry. HL-60 human acute promyelocytic leukemia cell line was stained with Goat Anti-Human WT1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5729, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Detection of Human WT1 by Immunocytochemistry/Immunofluorescence Immunostaining of podocyte-like cells derived from the iPSC line SBAD3 with podocyte markers and F-actin staining.iPSC were differentiated on glass cover slips fixed and stained for synaptopodin, WT-1, podocin, and F-actin as described in methods. Red and green colours were applied post capture. Staining in two other iPSC lines are provided in S1 and S2 Figs. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30222766), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Human WT1 by Immunocytochemistry/Immunofluorescence Immunostaining of podocyte-like cells derived from the iPSC line SBAD3 with podocyte markers and F-actin staining.iPSC were differentiated on glass cover slips fixed and stained for synaptopodin, WT-1, podocin, and F-actin as described in methods. Red and green colours were applied post capture. Staining in two other iPSC lines are provided in S1 and S2 Figs. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30222766), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Human WT1 by Western Blot Immunostaining of podocyte-like cells derived from the iPSC line SBAD3 with podocyte markers and F-actin staining.iPSC were differentiated on glass cover slips fixed and stained for synaptopodin, WT-1, podocin, and F-actin as described in methods. Red and green colours were applied post capture. Staining in two other iPSC lines are provided in S1 and S2 Figs. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30222766), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Human WT1 by Immunocytochemistry/Immunofluorescence Immunostaining of podocyte-like cells derived from the iPSC line SBAD3 with podocyte markers and F-actin staining.iPSC were differentiated on glass cover slips fixed and stained for synaptopodin, WT-1, podocin, and F-actin as described in methods. Red and green colours were applied post capture. Staining in two other iPSC lines are provided in S1 and S2 Figs. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30222766), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Human WT1 by Immunocytochemistry/Immunofluorescence Differentiation of human iPSC clone IV towards renal commitment.(a) Representative immunofluorescence images of co-staining for Lhx1/Osr1 up to day 6. (b) Images of co-staining of Pax2/Six2, Pax8/Six2 and Six2/Wt1 from day 6 to 12. (c) Expression of renal progenitor markers such as CD24, Claudin1 and GGT1 from day 0 to 19. Nuclei are stained with DAPI (blue). Scale bars: 50 μm (a, b), 20 μm (c). (d) Gene expression analysis for renal progenitor markers at different points in time. Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/srep08826), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Human WT1 by Immunocytochemistry/Immunofluorescence Immunostaining of podocyte-like cells derived from the iPSC line SBAD3 with podocyte markers and F-actin staining.iPSC were differentiated on glass cover slips fixed and stained for synaptopodin, WT-1, podocin, and F-actin as described in methods. Red and green colours were applied post capture. Staining in two other iPSC lines are provided in S1 and S2 Figs. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30222766), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Human WT1 by Immunocytochemistry/Immunofluorescence Stepwise differentiation of human iPSCs towards renal progenitor cells (RPCs).(a) Schematic description of the two-stage protocol applied to iPSC-renal commitment. (b, c, d, e) Immunofluorescence of iPSCs (derived from retroviral transfected dermal fibroblasts) exposed to differentiating media. (b) The pluripotency markers SSEA4, TRA-1-81, Nanog, ME marker T(Bry) and IM marker Osr1. (c) IM and MM marker expression as Wt1, Pax8, Pax2, Six2 and Sall1. (d) Renal progenitor markers CD133, CD24, NCAM, glomerular epithelial marker Claudin1 and proximal tubular epithelial markers AQP1, GGT1. (e) Markers identifying endodermal AFP, ectodermal Pax6, and cardiac mesodermal Nkx2.5 derivation. Nuclei are stained with DAPI (blue). Scale bars: 20 μm (b, c, d, e). Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/srep08826), licensed under a CC-BY license. Not internally tested by R&D Systems.