Human ZBP1/DLM-1/DAI Antibody Summary
Ala2-Ile429
Accession # Q9H171
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human ZBP1/DLM‑1/DAI by Western Blot. Western blot shows lysates of human peripheral blood lymphocytes (PBL) and human tonsil tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human ZBP1/DLM-1/DAI Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6309) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for ZBP1/DLM-1/DAI at approximately 30-60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: ZBP1/DLM-1/DAI
ZBP1 (Z-DNA binding protein 1; also DAI and DLM-1) is a 15-60 kDa cytosolic detector of intracellular DNA. In a TOLL-independent manner, ZBP1 detects cytoplasmic dsDNA and initiates INF-beta production. It does so by binding DNA, followed by multimerization and recruitment of TBK1 and IRF3. This is accompanied by a parallel interaction with RIP-1 and -3 that stimulates NF kappa B activity and cytokine production. Human ZBP1 is 429 amino acids (aa) in length. It contains two DNA-binding Z-regions (aa 6-72 and 103-168) plus a third DNA-binding region that likely lies in the region between aa 170-250. Multiple splice forms exist. One shows a deletion of aa 12-86, a second shows a 63 aa substitution for aa 87-429, a third contains a 24 aa substitution for aa 225-429, a fourth reveals a deletion of aa 87-109, while a fifth and sixth show 82 and 16 aa substitutions for aa 365-429, respectively. Full length human ZBP1 shares only 47% aa identity with mouse ZBP1.
Product Datasheets
Citations for Human ZBP1/DLM-1/DAI Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 3
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ZBP1-dependent inflammatory cell death, PANoptosis, and cytokine storm disrupt IFN therapeutic efficacy during coronavirus infection
Authors: Karki R, Lee S, Mall R et al.
Science immunology
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ZBP1 not RIPK1 mediates tumor necroptosis in breast cancer
Authors: JY Baik, Z Liu, D Jiao, HJ Kwon, J Yan, C Kadigamuwa, M Choe, R Lake, M Kruhlak, M Tandon, Z Cai, S Choksi, ZG Liu
Nature Communications, 2021-05-11;12(1):2666.
Species: Mouse
Sample Types: Tissue Homogenates, Whole Tissue
Applications: IHC, Western Blot -
ABT?737, a Bcl?2 family inhibitor, has a synergistic effect with apoptosis by inducing urothelial carcinoma cell necroptosis
Authors: R Cheng, X Liu, Z Wang, K Tang
Molecular Medicine Reports, 2021-03-31;23(6):.
Species: Human
Sample Types: Cell Lysates
Applications: Co-Immunoprecipitation, Western Blot
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