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JQ1-FITC

Catalog #: 7722
1 Citation Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
7722/2
JQ1-FITC | Fluorescent Probes
1 Image
Description: Fluorescent BET bromodomain probe; suitable for use in TR-FRET

Chemical Name: (S)-5-(3-(1-(4-(4-Chlorophenyl)-2,3,9-trimethyl-6H-thieno[3,2-f][1,2,4]triazolo[4,3-a][1,4]diazepin-6-yl)-2-oxo-7,10,13-trioxa-3-azahexadecan-16-yl)thioureido)-2-(6-hydroxy-3-oxo-3H-xanthen-9-yl)benzoic acid trifluoroacetate

Purity: ≥95%

Product Details
Citations (1)
Reviews
Biological Activity Technical Data Product Datasheets Calculators

Biological Activity

JQ1-FITC is a fluorescent BET bromodomain probe. It binds with high affinity to BRD4(BD1) and BRD4(BD2) (KD,app values are 6.5 nM and 5.8 nM, respectively, using GST-tagged BRD4 proteins measured by CoraFluor™ TR-FRET assay; 58.7 nM for BRD4(BD1) in a fluorescence anisotropy assay). Excitation and emission maxima (λ) are 495 nm and 525 nm.

Optical Data for JQ1-FITC

JQ1-FITC Dye Spectra
Emission Color Green
λabs 495 nm
λem 525 nm
Extinction Coefficient (ε) 7500 M-1cm-1
Quantum Yield (φ) 0.92
Closest Laser line 488 nm
Application TR-FRET, Fluorescence polarization assays
Alternative For FITC

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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Technical Data

M.Wt:
1106.58
Formula:
C50H50ClN7O9S2.CF3CO2H
Solubility:
Soluble to 10 mM in DMSO
Purity:
≥95%
Storage:
Store at -20°C

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.

Product Datasheets

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Citation for JQ1-FITC

The citations listed below are publications that use Tocris products. Selected citations for JQ1-FITC include:

1 Citation: Showing 1 - 1

  1. Protocol for the comprehensive biochemical and cellular profiling of small-molecule degraders using CoraFluor TR-FRET technology.
    Authors: Payne Et al.
    STAR Protoc.  2024;5:103129

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