When using MacCellect Cell Isolation kits (MAGM*** or MAGH***), why is it important to lyse red blood cells before starting the cell isolation protocol?

Name: MagCellect Plus Human EpCAM+ Cell Isolation Kit
Brand: R&D Systems
SKU: MAGH121
Price: 809 USD

It is important to remove red blood cells before the isolation protocol using MagCellect isolation kits because not doing so can cause a poorer yield of selected cells. Red blood cells become sticky during the incubation protocols and may interfere with the interactions needed for removal of unwanted populations.

MagCellect Plus Human EpCAM+ Cell Isolation Kit

Catalog #: MAGH121 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAGH121

R&D Systems Magcellect Cell Isolation Kits

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MagCellect Plus Human EpCAM+ Cell Isolation Kit Summary

Specifications

Source

N/A

Shipping Conditions

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Species

Human

Product Datasheets

Product Datasheet

COA

SDS

Background: EpCAM/TROP1

Epithelial Cellular Adhesion Molecule (EpCAM), also known as TROP 1, is a calcium-dependent homophilic adhesion protein. It associates into tetramers and forms complexes in cis with Claudin-7, CD44v6, TSPAN8, CD9, Integrin alpha 3, and Annexin A1. During embryonic development, EpCAM is expressed in lung, kidney, liver, pancreas, skin, and germ cells. In the adult, it is expressed on basolateral cell membranes of simple, pseudo-stratified, and transitional epithelia. EpCAM is upregulated in proliferating epithelial tissues, during adult liver regeneration, and many carcinomas.

Long Name

Epithelial Cell Adhesion Molecule

Entrez Gene IDs

4072 (Human); 17075 (Mouse); 171577 (Rat); 102123042 (Cynomolgus Monkey)

Alternate Names

17-1A; 323/A3; ACSTD1; antigen identified by monoclonal AUA1; CD326 antigen; CD326; Cell surface glycoprotein Trop-1; chromosome 4, surface marker (35kD glycoprotein); DIAR5; EGP; EGP-2; EGP314; EGP40; EpCAM; epithelial cell adhesion molecule; Epithelial cell surface antigen; Epithelial glycoprotein 314; Epithelial glycoprotein; ESA; GA733-2; GA733-2EGP34; gp40; hEGP314; HNPCC8; KS 1/4 antigen; KS1/4; KSAHEA125; M1S2; M4S1; M4S1Ly74; Major gastrointestinal tumor-associated protein GA733-2; MIC18MH99; MOC31; TACST-1; TACSTD1; TROP1; TROP1CD326; Tumor-associated calcium signal transducer 1CO-17A

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FAQs

The MagCellect Plus Human EpCAM+ Cell Isolation kit, Catalog # MAGH121, lists Hank's BSS (HBSS) or equivalent is required. Which HBSS do you recommend?
- We recommned using HBSS with Ca+ and Mg+ salts, and no phenol red, such as R&D Systems Catalog # B32450.
When using MacCellect Cell Isolation kits (MAGM*** or MAGH***), why is it important to lyse red blood cells before starting the cell isolation protocol?
- It is important to remove red blood cells before the isolation protocol using MagCellect isolation kits because not doing so can cause a poorer yield of selected cells. Red blood cells become sticky during the incubation protocols and may interfere with the interactions needed for removal of unwanted populations.

View all Cell Selection and Detection Kit FAQs