Mouse A33 Antibody

Catalog #: AF2756
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF2756
AF2756-SP

All A33 Antibodies

Product Details

Citations (1)

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse A33 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse A33 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 15% cross-reactivity with recombinant human A33 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse A33
Leu22-Ile235
Accession # Q9JKA5

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Mouse A33

Immunohistochemistry

5-15 µg/mL

Perfusion fixed frozen sections of mouse intestine

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: A33

Mouse A33, also known as Gpa33, is a 48 kDa type I transmembrane protein belonging to the CTX (cortical thymocyte marker in Xenopus) family of cell-adhesion molecules within the immunoglobulin superfamily. Other family members include CXADR, ESAM, BT-IgSF, CD2 and JAM-A-C. The extracellular region of A33 contains a V-type and a C2-type Ig-like domain. A33 is believed to be involved in cell-cell adhesion, either between epithelium or between epithelium and T cells. The extracellular domain of mouse A33 shares 87% and 71% amino acid sequence identity with the extracellular region of rat and human A33, respectively.

Long Name

Cell Surface A33 Antigen

Entrez Gene IDs

10223 (Human); 59290 (Mouse)

Alternate Names

A33; cell surface A33 antigen; glycoprotein A33 (transmembrane); Glycoprotein A33; GPA33; MGC129986; MGC129987; transmembrane glycoprotein A33

Product Datasheets

Product Datasheet

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Related Research Areas

Citation for Mouse A33 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

Prion Uptake in the Gut: Identification of the First Uptake and Replication Sites
Authors: Pekka Kujala, Claudine R. Raymond, Martijn Romeijn, Susan F. Godsave, Sander I. van Kasteren, Holger Wille et al.
PLoS Pathogens