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Mouse Cathepsin D Antibody

Catalog #: MAB1029
14 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB1029
MAB1029-SP
Product Details
Citations (14)
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Cathepsin D Antibody Summary

Species Reactivity
Mouse
Specificity
Detects both the pro and active forms of mouse Cathepsin D in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human Cathepsin D or recombinant mouse Cathepsin E is observed.
Source
Monoclonal Rat IgG2A Clone # 204712
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Cathepsin D
Ile21-Leu410
Accession # P18242
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Mouse Cathepsin D (Catalog # 1029-AS)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Cathepsin D

Cathepsin D is a lysosomal aspartic protease of the pepsin family (4). Mouse Cathepsin D is synthesized as a precursor protein, consisting of a signal peptide (residues 1‑20), a propeptide (residues 21‑64), and a mature chain (residues 65‑410) (1‑3). It is expressed in most cells and overexpressed in breast cancer cells (5). It is a major enzyme in protein degradation in lysosomes, and also involved in the presentation of antigenic peptides. Mice deficient in this enzyme showed a progressive atrophy of the intestinal mucosa, a massive destruction of lymphoid organs, and a profound neuronal ceroid lipofucinosis, indicating that Cathepsin D is essential for proteolysis of proteins regulating cell growth and tissue homeostasis (6). Cathepsin D secreted from human prostate carcinoma cells is responsible for the generation of angiostatin, a potent endogeneous inhibitor of angiogenesis (6).

References
  1. Diedrich, et al. (1990) Nucl. Acid Res. 18:7184.
  2. Grusby, et al. (1990) Nucl. Acid Res. 18:4008. 
  3. Hetman, et al. (1994) DNA Cell Biol. 13:419.
  4. Conner (2004) in Handbook of Proteolytic Enzymes (Barrett, et al. eds) Elsevier Academic Press, San Diego, p. 43.
  5. Rochefort, et al. (2000) Clin. Chim. Acta. 291:157.
  6. Tsukuba, et al. (2000) Mol. Cells 10:601.
Entrez Gene IDs
1509 (Human); 13033 (Mouse)
Alternate Names
cathepsin D (lysosomal aspartyl protease); Cathepsin D; CPSD; CTSD; EC 3.4.23; EC 3.4.23.5; lysosomal aspartyl peptidase; lysosomal aspartyl protease; MGC2311; neuronal 10

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Citations for Mouse Cathepsin D Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

14 Citations: Showing 1 - 10
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  1. Lipid-mediated motor-adaptor sequestration impairs axonal lysosome delivery leading to autophagic stress and dystrophy in Niemann-Pick type C
    Authors: Roney JC, Li S, Farfel-Becker T et al.
    Developmental cell
  2. Genetic disruption of WASHC4 drives endo-lysosomal dysfunction and cognitive-movement impairments in mice and humans
    Authors: Jamie L Courtland, Tyler WA Bradshaw, Greg Waitt, Erik J Soderblom, Tricia Ho, Anna Rajab et al.
    eLife
  3. RREB1 regulates neuronal proteostasis and the microtubule network
    Authors: Griffin, EN;Jucius, T;Sim, SE;Harris, BS;Heinz, S;Ackerman, SL;
    Science advances
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Immunohistochemistry
  4. Cathepsin D interacts with adenosine A2A receptors in mouse macrophages to modulate cell surface localization and inflammatory signaling
    Authors: A Skopál, T Kéki, PÁ Tóth, B Csóka, B Koscsó, ZH Német, L Antonioli, A Ivessa, F Ciruela, L Virág, G Haskó, E Kókai
    The Journal of Biological Chemistry, 2022-03-31;0(0):101888.
    Species: Mouse
    Sample Types: Cell Lysates, Whole Cells
    Applications: Western Blot, ICC
  5. Lysosome repositioning as an autophagy escape mechanism by Mycobacterium tuberculosis Beijing strain
    Authors: T Laopanupon, P Prombutara, P Kanjanasir, S Benjaskull, A Boonmee, T Palaga, S Méresse, J Paha, TAP Siregar, T Khumpanied, S Borwornpin, A Chaipraser, P Utaisincha, M Ponpuak
    Scientific Reports, 2021-02-22;11(1):4342.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  6. Retromer stabilization results in neuroprotection in a model of Amyotrophic Lateral Sclerosis
    Authors: L Muzio, R Sirtori, D Gornati, S Eleuteri, A Fossaghi, D Brancaccio, L Manzoni, L Ottoboni, L Feo, A Quattrini, E Mastrangel, L Sorrentino, E Scalone, G Comi, L Marinelli, N Riva, M Milani, P Seneci, G Martino
    Nat Commun, 2020-07-31;11(1):3848.
    Species: Mouse
    Sample Types: Whole Cells, Whole Tissue
    Applications: ICC, IHC
  7. Neuronal Soma-Derived Degradative Lysosomes Are Continuously Delivered to Distal Axons to Maintain Local Degradation Capacity
    Authors: T Farfel-Bec, JC Roney, XT Cheng, S Li, SR Cuddy, ZH Sheng
    Cell Rep, 2019-07-02;28(1):51-64.e4.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  8. Lack of Cathepsin D in the Renal Proximal Tubular Cells Resulted in Increased Sensitivity against Renal Ischemia/Reperfusion Injury
    Authors: C Suzuki, I Tanida, M Ohmuraya, JA Oliva Trej, S Kakuta, T Sunabori, Y Uchiyama
    Int J Mol Sci, 2019-04-05;20(7):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  9. Activation of autophagy rescues synaptic and cognitive deficits in fragile X mice
    Authors: J Yan, MW Porch, B Court-Vazq, MVL Bennett, RS Zukin
    Proc. Natl. Acad. Sci. U.S.A., 2018-09-21;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  10. Characterization of LAMP1-labeled nondegradative lysosomal and endocytic compartments in neurons
    Authors: XT Cheng, YX Xie, B Zhou, N Huang, T Farfel-Bec, ZH Sheng
    J. Cell Biol., 2018-04-25;0(0):.
    Species: Rat
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  11. GDE2 is essential for neuronal survival in the postnatal mammalian spinal cord
    Authors: C Cave, S Park, M Rodriguez, M Nakamura, A Hoke, M Pletnikov, S Sockanatha
    Mol Neurodegener, 2017-01-19;12(1):8.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  12. Cleavage of Histone 3 by Cathepsin D in the involuting mammary gland.
    Authors: Khalkhali-Ellis, Zhila, Goossens, William, Margaryan, Naira V, Hendrix, Mary J C
    PLoS ONE, 2014-07-23;9(7):e103230.
    Species: Human
    Sample Types: Tissue Homogenates, Whole Tissue
    Applications: IHC-Fr, Western Blot
  13. The iron export protein ferroportin 1 is differentially expressed in mouse macrophage populations and is present in the mycobacterial-containing phagosome.
    Authors: Van Zandt KE, Sow FB, Florence WC, Zwilling BS, Satoskar AR, Schlesinger LS, Lafuse WP
    J. Leukoc. Biol., 2008-06-27;84(3):689-700.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  14. Securinine, a GABAA receptor antagonist, enhances macrophage clearance of phase II C. burnetii: comparison with TLR agonists.
    Authors: Lubick K, Radke M, Jutila M
    J. Leukoc. Biol., 2007-08-14;82(5):1062-9.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC

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