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Mouse CCR3 APC-conjugated Antibody

Catalog #: FAB729A
3 Citations Datasheet / COA / SDS

Discontinued Product

FAB729A has been discontinued.
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Detection of CCR3 in Mouse Peripheral Blood Cells by Flow Cytometry.
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Product Details
Citations (3)
FAQs
Reviews
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse CCR3 APC-conjugated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse CCR3. Specifically stains mouse CCR3 transfectants but not the parental cell lines. Does not cross‑react with Y3 cells transfected with human CCR3.
Source
Monoclonal Rat IgG2A Clone # 83101
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Y3 rat myeloid cell line transfected with mouse CCR3
Met1-Phe359
Accession # P51678
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25-1 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of CCR3 antibody in Mouse Peripheral Blood Cells antibody by Flow Cytometry. View Larger

Detection of CCR3 in Mouse Peripheral Blood Cells by Flow Cytometry. Mouse peripheral blood cells were stained with Rat Anti-Mouse Gr-1/Ly-6G PE-conjugated Monoclonal Antibody (Catalog # FAB1037P) and either (A) Rat Anti-Mouse CCR3 APC-conjugated Monoclonal Antibody (Catalog # FAB729A) or (B) Rat IgG2AAllophycocyanin Isotype Control (Catalog # IC006A). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CCR3

CCR3 is a G protein-linked seven transmembrane receptor that is activated by several different chemokines. It is expressed on subsets of granulocytes, monocytes, T cells and epithelial cells.

Entrez Gene IDs
1232 (Human); 12771 (Mouse); 117027 (Rat); 448802 (Canine)
Alternate Names
b-chemokine receptor; CC chemokine receptor 3; C-C chemokine receptor type 3; C-C CKR-3; CCR3; CCR-3; CD193 antigen; CD193; chemokine (C-C motif) receptor 3; CKR3CC-CKR-3CMKBR3CD193; eosinophil CC chemokine receptor 3; Eosinophil eotaxin receptor; MGC102841

Product Datasheets

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Citations for Mouse CCR3 APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Eosinophils regulate peripheral B cell numbers in both mice and humans.
    Authors: Wong, Tina W, Doyle, Alfred D, Lee, James J, Jelinek, Diane F
    J Immunol, 2014-03-10;192(8):3548-58.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. The adaptor protein CIKS/Act1 is essential for IL-25-mediated allergic airway inflammation.
    Authors: Claudio E, Sonder SU, Saret S, Carvalho G, Ramalingam TR, Wynn TA, Chariot A, Garcia-Perganeda A, Leonardi A, Paun A, Chen A, Ren NY, Wang H, Siebenlist U
    J. Immunol., 2009-02-01;182(3):1617-30.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Skewing of cytotoxic activity and chemokine production, but not of chemokine receptor expression, in human type-1/-2 gamma delta T lymphocytes.
    Authors: D'Ambrosio D
    Eur. J. Immunol., 2002-10-01;32(10):2934-43.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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