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Mouse CCR3 PE-conjugated Antibody

Catalog #: FAB729P
17 Citations 1 Review Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
FAB729P-100UG
FAB729P-025UG
Detection of CCR3 in Mouse Splenocytes by Flow Cytometry.
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Product Details
Citations (17)
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Supplemental Products
Reviews (1)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse CCR3 PE-conjugated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse CCR3. Specifically stains mouse CCR3 transfectants but not the parental cell lines. Does not cross‑react with Y3 cells transfected with human CCR3.
Source
Monoclonal Rat IgG2A Clone # 83101
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Y3 rat myeloid cell line transfected with mouse CCR3
Met1-Phe359
Accession # P51678
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25-1 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of CCR3 antibody in Mouse Splenocytes antibody by Flow Cytometry. View Larger

Detection of CCR3 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse CCR3 PE-conjugated Monoclonal Antibody (Catalog # FAB729P) and Rat Anti-Mouse Gr-1/Ly-6G Fluoresein-conjugated Monoclonal Antibody (Catalog # FAB1037F). Quadrant markers were set based on control antibody staining (Catalog # IC006P). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CCR3

CCR3 is a G protein-linked seven transmembrane receptor that is activated by several different chemokines. It is expressed on subsets of granulocytes, monocytes, T cells and epithelial cells.

Entrez Gene IDs
1232 (Human); 12771 (Mouse); 117027 (Rat); 448802 (Canine)
Alternate Names
b-chemokine receptor; CC chemokine receptor 3; C-C chemokine receptor type 3; C-C CKR-3; CCR3; CCR-3; CD193 antigen; CD193; chemokine (C-C motif) receptor 3; CKR3CC-CKR-3CMKBR3CD193; eosinophil CC chemokine receptor 3; Eosinophil eotaxin receptor; MGC102841

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Citations for Mouse CCR3 PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

17 Citations: Showing 1 - 10
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  1. Pyruvate kinase M2 in lung APCs regulates Alternaria-induced airway inflammation
    Authors: Jaiswal AK, Makhija S, Stahr N Et al.
    Immunobiology
  2. Rapid GPR183-mediated recruitment of eosinophils to the lung after Mycobacterium tuberculosis infection
    Authors: Bohrer AC, Castro E, Tocheny CE Et al.
    Cell Rep
  3. Genetic deletion of HVEM in a leukemia B cell line promotes a preferential increase of PD-1- stem cell-like T cells over PD-1+ T cells curbing tumor progression
    Authors: Maria-Luisa del Rio, Carla Yago-Diez de Juan, Giovanna Roncador, Eduardo Caleiras, Ramón Álvarez-Esteban, José Antonio Pérez-Simón et al.
    Frontiers in Immunology
  4. The protective role of conjunctival goblet cell mucin sialylation
    Authors: M Matsuzawa, T Ando, S Fukase, M Kimura, Y Kume, T Ide, K Izawa, A Kaitani, M Hara, E Nakamura, A Kamei, A Matsuda, N Nakano, K Maeda, N Tada, H Ogawa, K Okumura, A Murakami, N Ebihara, J Kitaura
    Nature Communications, 2023-03-17;14(1):1417.
  5. A nerve-goblet cell association promotes allergic conjunctivitis through the rapid antigen passage
    Authors: Kimura, M;Ando, T;Kume, Y;Fukase, S;Matsuzawa, M;Kashiwagi, K;Izawa, K;Kaitani, A;Nakano, N;Maeda, K;Ogawa, H;Okumura, K;Nakao, S;Murakami, A;Ebihara, N;Kitaura, J;
    JCI insight
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. Allergen Delivery Inhibitors: Characterisation of Potent and Selective Inhibitors of Der p 1 and Their Attenuation of Airway Responses to House Dust Mite Allergens
    Authors: J Zhang, J Chen, J Zuo, GK Newton, MR Stewart, TR Perrior, DR Garrod, C Robinson
    Int J Mol Sci, 2018-10-15;19(10):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. A prominent air pollutant, Indeno[1,2,3-cd]pyrene, enhances allergic lung inflammation via aryl hydrocarbon receptor
    Authors: TH Wong, CL Lee, HH Su, CL Lee, CC Wu, CC Wang, CC Sheu, RS Lai, SY Leung, CC Lin, YF Wei, CJ Wang, YC Lin, HL Chen, MS Huang, JH Yen, SK Huang, JL Suen
    Sci Rep, 2018-03-26;8(1):5198.
    Species: Mouse
    Sample Types: BALF
    Applications: Flow Cytometry
  8. Self-renewal and phenotypic conversion are the main physiological responses of macrophages to the endogenous estrogen surge
    Authors: G Pepe, D Braga, TA Renzi, A Villa, C Bolego, F D'Avila, C Barlassina, A Maggi, M Locati, E Vegeto
    Sci Rep, 2017-03-20;7(0):44270.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  9. CCL2/CCR2 Regulates the Tumor Microenvironment in HER-2/neu-Driven Mammary Carcinomas in Mice
    PLoS ONE, 2016-11-07;11(11):e0165595.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  10. Over-expression of the LTC4 synthase gene in mice reproduces human aspirin-induced asthma.
    Authors: Hirata H, Arima M, Fukushima Y, Honda K, Sugiyama K, Tokuhisa T, Fukuda T
    Clin. Exp. Allergy, 2011-03-24;41(8):1133-42.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  11. Impaired basophil induction leads to an age-dependent innate defect in type 2 immunity during helminth infection in mice.
    Authors: Nel HJ, Hams E, Saunders SP
    J. Immunol., 2011-03-11;186(8):4631-9.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  12. The TLR7 ligand 9-benzyl-2-butoxy-8-hydroxy adenine inhibits IL-17 response by eliciting IL-10 and IL-10-inducing cytokines.
    Authors: Vultaggio A, Nencini F, Pratesi S, Maggi L, Guarna A, Annunziato F, Romagnani S, Parronchi P, Maggi E
    J. Immunol., 2011-03-09;186(8):4707-15.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  13. Anti-Siglec-F antibody reduces allergen-induced eosinophilic inflammation and airway remodeling.
    Authors: Song DJ, Cho JY, Lee SY, Miller M, Rosenthal P, Soroosh P, Croft M, Zhang M, Varki A, Broide DH
    J. Immunol., 2009-09-25;183(8):5333-41.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  14. Ablation of type I hypersensitivity in experimental allergic conjunctivitis by eotaxin-1/CCR3 blockade.
    Authors: Miyazaki D, Nakamura T, Ohbayashi M, Kuo CH, Komatsu N, Yakura K, Tominaga T, Inoue Y, Higashi H, Murata M, Takeda S, Fukushima A, Liu FT, Rothenberg ME, Ono SJ
    Int. Immunol., 2009-01-15;21(2):187-201.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  15. Sensitization by intratracheally injected dendritic cells is independent of antigen presentation by host antigen-presenting cells.
    Authors: Kuipers H, Soullie T, Hammad H, Willart M, Kool M, Hijdra D, Hoogsteden HC, Lambrecht BN
    J. Leukoc. Biol., 2008-10-15;85(1):64-70.
    Species: Mouse
    Sample Types: BALF
    Applications: Flow Cytometry
  16. Treatment with anti-CC chemokine receptor 3 monoclonal antibody or dexamethasone inhibits the migration and differentiation of bone marrow CD34 progenitor cells in an allergic mouse model.
    Authors: Ben S, Li X, Xu F, Xu W, Li W, Wu Z, Huang H, Shi H, Shen H
    Allergy, 2008-09-01;63(9):1164-76.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  17. Protease-activated receptor-2 signaling through beta -arrestin-2 mediates Alternaria alkaline serine protease-induced airway inflammation
    Authors: Michael C. Yee, Heddie L. Nichols, Danny Polley, Mahmoud Saifeddine, Kasturi Pal, Kyu Lee et al.
    American Journal of Physiology-Lung Cellular and Molecular Physiology

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Reviews for Mouse CCR3 PE-conjugated Antibody

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Mouse CCR3 PE-conjugated Antibody
By Anonymous on 08/14/2016
Application: Flow Sample Tested: Bronchoalveolar lavage Species: Mouse
Flow Cytometry Mouse CCR3 PE-conjugated Antibody FAB729P