Mouse CD200 R1 APC-conjugated Antibody
Mouse CD200 R1 APC-conjugated Antibody Summary
Thr26-Pro238
Accession # BAE42266
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of CD200 R1 in MC/9‑2 Mouse Cell Line by Flow Cytometry. MC/9-2 mouse mast cell line was stained with Goat Anti-Mouse CD200 R1 APC-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB2554A, filled histogram) or isotype control antibody (Catalog # IC108A, open histogram). View our protocol for Staining Membrane-associated Proteins.
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Background: CD200R1
CD200 R1, also known as OX-2 receptor, is a 90 kDa, type I transmembrane protein that belongs to the immunoglobulin superfamily. CD200 R1 is important in the regulation of myeloid cell activity (1‑3). The mouse CD200 R1 cDNA encodes a 326 aa precursor that includes a 25 aa signal sequence, a 213 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 67 aa cytoplasmic domain. The ECD is composed of one Ig-like V-type domain and one Ig-like C2-type domain (4). Within the ECD, mouse CD200 R1 shares 56% and 70% aa sequence identity with human and rat CD200 R1, respectively. The ECD of mouse CD200 R1 shares 69%, 38%, 79%, and 83% aa sequence identity with the ECD of CD200 R2, 3, 4, and a CD200 R-like molecule, respectively. CD200 R1 is expressed primarily on mast cells, basophils, macrophages, and dendritic cells, (5‑7) while its ligand, CD200, is widely distributed (8). Disruption of this receptor-ligand pair by knockout of the CD200 gene leads to increased macrophage number and activation, plus a predisposition to autoimmune disorders (9). Association of CD200 with CD200 R1 takes place between their respective N-terminal Ig-like domains (10). The CD200 R-like molecules may interact differently with CD200 (11, 12). The cytoplasmic domain of CD200 R1 contains two non-ITIM tyrosine residues which are required for propagating its inhibitory signals (13‑15). CD200 R-like molecules, in contrast, are potentially activating receptors by means of their association with DAP12 (4, 16).
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- Wright, G.J. et al. (2001) Immunology 102:173.
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- Hatherley, D. and A.N. Barclay (2004) Eur. J. Immunol. 34:1688.
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- Gorczynski, R. et al. (2004) J. Immunol. 172:7744.
- Zhang, S. and J.H. Phillips (2006) J. Leukoc. Biol. 79:363.
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- Jenmalm, M.C. et al. (2006) J. Immunol. 176:191.
- Voehringer, D. et al. (2004) J. Biol. Chem. 279:54117.
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