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Mouse CD200R1 Antibody

Catalog #: AF2554
8 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF2554
AF2554-SP
CD200 R1 in Mouse Splenocytes.
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Citations (8)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse CD200R1 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse CD200 R1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross‑reactivity with recombinant human CD200 R is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse CD200 R1
Thr26-Pro238
Accession # BAE42266
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse CD200 R1 Fc Chimera (Catalog # 2554-CD)
Flow Cytometry
2.5 µg/106 cells
MC/9‑2 mouse masT cell line
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry CD200 R1 antibody in Mouse Splenocytes by Immunocytochemistry (ICC). View Larger

CD200 R1 in Mouse Splenocytes. CD200 R1 was detected in immersion fixed mouse splenocytes using Goat Anti-Mouse CD200 R1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2554) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD200R1

CD200 R1, also known as OX-2 receptor, is a 90 kDa, type I transmembrane protein that belongs to the immunoglobulin superfamily. CD200 R1 is important in the regulation of myeloid cell activity (1‑3). The mouse CD200 R1 cDNA encodes a 326 aa precursor that includes a 25 aa signal sequence, a 213 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 67 aa cytoplasmic domain. The ECD is composed of one Ig-like V-type domain and one Ig-like C2-type domain (4). Within the ECD, mouse CD200 R1 shares 56% and 70% aa sequence identity with human and rat CD200 R1, respectively. The ECD of mouse CD200 R1 shares 69%, 38%, 79%, and 83% aa sequence identity with the ECD of CD200 R2, 3, 4, and a CD200 R-like molecule, respectively. CD200 R1 is expressed primarily on mast cells, basophils, macrophages, and dendritic cells, (5‑7) while its ligand, CD200, is widely distributed (8). Disruption of this receptor-ligand pair by knockout of the CD200 gene leads to increased macrophage number and activation, plus a predisposition to autoimmune disorders (9). Association of CD200 with CD200 R1 takes place between their respective N-terminal Ig-like domains (10). The CD200 R-like molecules may interact differently with CD200 (11, 12). The cytoplasmic domain of CD200 R1 contains two non-ITIM tyrosine residues which are required for propagating its inhibitory signals (13‑15). CD200 R-like molecules, in contrast, are potentially activating receptors by means of their association with DAP12 (4, 16).

References
  1. Rosenblum, M.D. et al. (2006) J. Dermatol. Sci. 41:165.
  2. Gorczynski, R.M. (2005) Curr. Opin. Invest. Drugs 6:483. 
  3. Barclay, A.N. et al. (2002) Trends Immunol. 23:285. 
  4. Wright, G.J. et al. (2003) J. Immunol. 171:3034. 
  5. Shiratori, I. et al. (2005) J. Immunol. 175:4441. 
  6. Cherwinski, H.M. et al. (2005) J. Immunol. 174:1348. 
  7. Fallarino, F. et al. (2004) J. Immunol. 173:3748. 
  8. Wright, G.J. et al. (2001) Immunology 102:173. 
  9. Hoek, R.M. et al. (2000) Science 290:1768.
  10. Hatherley, D. and A.N. Barclay (2004) Eur. J. Immunol. 34:1688.
  11. Hatherley, D. et al. (2005) J. Immunol. 175:2469.
  12. Gorczynski, R. et al. (2004) J. Immunol. 172:7744.
  13. Zhang, S. and J.H. Phillips (2006) J. Leukoc. Biol. 79:363.
  14. Zhang, S. et al. (2004) J. Immunol. 173:6786.
  15. Jenmalm, M.C. et al. (2006) J. Immunol. 176:191.
  16. Voehringer, D. et al. (2004) J. Biol. Chem. 279:54117.
Long Name
CD200 Receptor 1
Entrez Gene IDs
131450 (Human); 57781 (Mouse); 102139962 (Cynomolgus Monkey)
Alternate Names
CD200 R1; CD200 receptor 1; CD200R1; CD200RMOX2Rcell surface glycoprotein CD200 receptor 1; Cell surface glycoprotein OX2 receptor 1; cell surface glycoprotein receptor CD200; CRTR2; HCRTR2; MOX2 receptor; MOX2R; OX2RCD200 cell surface glycoprotein receptor

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Citations for Mouse CD200R1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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  1. MicroRNA-129-5p-regulated microglial expression of the surface receptor CD200R1 controls neuroinflammation
    Authors: V Singh, S Kushwaha, JA Ansari, S Gangopadhy, SK Mishra, RK Dey, AK Giri, S Patnaik, D Ghosh
    The Journal of Biological Chemistry, 2021-12-22;0(0):101521.
    Species: Mouse
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Western Blot
  2. Cochlear supporting cells function as macrophage-like cells and protect audiosensory receptor hair cells from pathogens
    Authors: Y Hayashi, H Suzuki, W Nakajima, I Uehara, A Tanimura, T Himeda, S Koike, T Katsuno, SI Kitajiri, N Koyanagi, Y Kawaguchi, K Onomoto, H Kato, M Yoneyama, T Fujita, N Tanaka
    Sci Rep, 2020-04-21;10(1):6740.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  3. CD200Fc Improves Neurological Function by Protecting the Blood-brain Barrier after Intracerebral Hemorrhage
    Authors: CS Le, XD Hao, JW Li, JW Zhong, HR Lin, YT Zhou, ZD Travis, LS Tong, F Gao
    Cell Transplant, 2019-06-17;0(0):9636897198576.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  4. Adipose-Derived Neural Stem Cells Combined with Acellular Dermal Matrix as a Neural Conduit Enhances Peripheral Nerve Repair
    Authors: WZ Syu, DY Hueng, WL Chen, JY Chan, SG Chen, SM Huang
    Cell Transplant, 2019-05-31;0(0):9636897198535.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  5. DNER modulates the length, polarity and synaptogenesis of spiral ganglion neurons via the Notch signaling pathway
    Authors: J Du, X Wang, X Zhang, X Zhang, H Jiang
    Mol Med Rep, 2018;17(2):2357-2365.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  6. CD200R/Foxp3-mediated signalling regulates microglial activation.
    Authors: Yi M, Zhang E, Kim J, Baek H, Shin N, Kim S, Kim S, Kim H, Lee S, Park J, Kim Y, Kwon O, Lee Y, Oh S, Kim D
    Sci Rep, 2016-10-12;6(0):34901.
    Species: Mouse, Rat
    Sample Types: Cell Lysates, Whole Tissue
    Applications: IHC, Western Blot
  7. Expression of CD200 in alternative activation of microglia following an excitotoxic lesion in the mouse hippocampus.
    Authors: Yi M, Zhang E, Kang J, Shin Y, Byun J, Oh S, Seo J, Lee Y, Kim D
    Brain Res, 2012-09-04;1481(0):90-6.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  8. The immunosuppressive surface ligand CD200 augments the metastatic capacity of squamous cell carcinoma.
    Authors: Stumpfova M, Ratner D, Desciak EB, Eliezri YD, Owens DM
    Cancer Res., 2010-03-23;70(7):2962-72.
    Species: Mouse
    Sample Types: Whole Cells, Whole Tissue
    Applications: Flow Cytometry, IHC-P

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