Mouse CD300a/LMIR1 Antibody

Catalog #: MAB1186
3 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB1186
MAB1186-SP

All CD300a/LMIR1 Antibodies

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Citations (3)

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse CD300a/LMIR1 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse CD300a/LMIR1 in direct ELISAs. In direct ELISAs, approximately 2% cross-reactivity with recombinant mouse (rm) LMIR2 is observed and no cross-reactivity with recombinant human LMIR1, 2, 3, 4, 5, 6, or rmLMIR3, 4, or 5 is observed.

Source

Monoclonal Rat IgG_2A Clone # 172224

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse CD300a/LMIR1
Leu28-Arg183
Accession # BAC80268

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

Mouse T1165 plasmocytoma cell line

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD300a/LMIR1

CD300a, also known as LMIR1, CMRF-35H, IRp60, CLM-8, and MAIR-I, is a 60 kDa glycoprotein member of the immunoglobulin superfamily (1). Mouse CD300a consists of a 158 amino acid (aa) extracellular domain (ECD) with one Ig-like V-type domain, a 21 aa transmembrane segment, and a 112 aa cytoplasmic domain that contains three immunoreceptor tyrosine-based inhibitory motifs (ITIMs) and a non-canonical ITIM (2, 3). Within the ECD, mouse CD300a shares 40% and 66% aa sequence identity with human and rat CD300a, respectively. Alternate splicing generates an additional mouse CD300a isoform with a 4 aa deletion following the Ig-like domain (3). In mouse, CD300a is expressed on peripheral eosinophils, mast cells, neutrophils, dendritic cells, macrophages, and some B cells (2‑4). Antibody cross‑linking of CD300a induces phosphorylation of tyrosine residues in the cytoplasmic domain. This leads to the recruitment of phosphatases SHIP, SHP-1, and SHP-2 and inhibition of NK cell, eosinophil, and mast cell activation (2, 3, 5‑7). Cross‑linking of CD300a to other surface proteins such as SCF R or Fc epsilon RI on mast cells, Fc gamma RIIA on neutrophils, or CCR3 on mast cells and eosinophils inhibits downstream signaling from those receptors (4, 8‑10). CD300a cross‑linking also limits the in vivo activities of these cells with a subsequent reduction of allergic inflammation symptoms (4, 7, 9).

References

Clark, G.J. et al. (2009) Trends Immunol. 30:209.
Kumagai, H. et al. (2003) Biochem. Biophys. Res. Commun. 307:719.
Yotsumoto, K. et al. (2003) J. Exp. Med. 198:223.
Munitz, A. et al. (2006) J. Allergy Clin. Immunol. 118:1082.
Cantoni, C. et al. (1999) Eur. J. Immunol. 29:3148.
Munitz, A. et al. (2006) Blood 107:1996.
Bachelet, I. et al. (2005) J. Immunol. 175:7989.
Bachelet, I. et al. (2008) J. Immunol. 180:6064.
Bachelet, I. et al. (2006) J. Allergy Clin. Immunol. 117:1314.
Alvarez, Y. et al. (2008) Mol. Immunol. 45:253.

Entrez Gene IDs

11314 (Human); 217303 (Mouse)

Alternate Names

CD300a antigenCMRF35H9; CD300a molecule; CD300a; CLM8; CLM-8; CMRF35H leukocyte immunoglobulin-like receptor; CMRF-35H; CMRF35-H; CMRF35-H9; CMRF-35-H9CD300 antigen-like family member A; CMRF35HIRp60; CMRF35-like molecule 8; IGSF12; IGSF12NK inhibitory receptor; Immunoglobulin superfamily member 12; Inhibitory receptor protein 60; IRC1; IRC1/IRC2; IRC2; IRp60; leukocyte membrane antigen; LMIR1; MAIR-I; Mcipr1; NKRL; Pigr4

Product Datasheets

Product Datasheet

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Citations for Mouse CD300a/LMIR1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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Leukocyte CD300a Contributes to the Resolution of Murine Allergic Inflammation
Authors: L Karra, R Singh Gang, R Shamri, PG Puzzovio, S Cohen-Mor, BD Levy, F Levi-Schaf
J. Immunol., 2018-10-12;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Engagement Assay, Flow Cytometry
CD300f:IL-5 cross-talk inhibits adipose tissue eosinophil homing and subsequent IL-4 production
Authors: P Rozenberg, H Reichman, I Zab-Bar, M Itan, M Pasmanik-C, C Bouffi, U Qimron, I Bachelet, PC Fulkerson, ME Rothenberg, A Munitz
Sci Rep, 2017-07-19;7(1):5922.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry
Mast cell costimulation by CD226/CD112 (DNAM-1/Nectin-2): a novel interface in the allergic process.
Authors: Bachelet I, Munitz A, Mankutad D, Levi-Schaffer F
J. Biol. Chem., 2006-07-10;281(37):27190-6.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry