Mouse CD300f/LMIR3 Antibody

Catalog #: AF2788
7 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF2788
AF2788-SP

All CD300f/LMIR3 Antibodies

Product Details

Citations (7)

Supplemental Products

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse CD300f/LMIR3 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse CD300f/LMIR3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human (rh) LMIR1, rhLMIR2, rhLMIR3, recombinant mouse LMIR5, and rhLMIR6 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse CD300f/LMIR3
Cys16-Gly188
Accession # Q6SJQ7

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Mouse CD300f/LMIR3

Flow Cytometry

2.5 µg/10⁶ cells

RAW 264.7 mouse monocyte/macrophage cell line

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD300f/LMIR3

Mouse CD300f, also known as LMIR3, CLM-1, IgSF13, IREM-1 and CMRF35-like molecule 1, is a 55 kDa type I transmembrane (TM) glycoprotein. It is synthesized as a 283 amino acid (aa) precursor that contains a putative 19 aa signal sequence, a 175 aa extracellular region that contains one Ig-like domain, a 17 aa TM segment and a 72 aa cytoplasmic domain that contains an ITIM sequence. There are multiple alleles for this gene that vary considerably in size. Mouse LMIR3 is expressed in monocytic cell lines. Mature mouse LMIR3 extracellular region shares 43% and 72% aa identity with human and rat LMIR3 extracellular region, respectively.

Long Name

Leukocyte Mono Ig-like Receptor 3

Entrez Gene IDs

146722 (Human); 246746 (Mouse)

Alternate Names

CMRF35-like molecule 1; CD300 molecule-like family member f; CD300f; CD300LF; CLIM1; CLM1; CLM-1; DIgR2; IGSF13; IREM1; IREM-1; LMIR3; NKIR; Pigr3

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Mouse CD300f/LMIR3 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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CD300LF Polymorphisms of Inbred Mouse Strains Confer Resistance to Murine Norovirus Infection in a Cell Type-Dependent Manner
Authors: Kevin Furlong, Scott B. Biering, Jayoung Choi, Craig B. Wilen, Robert C. Orchard, Christiane E. Wobus et al.
Journal of Virology
Increased hippocampal excitability in miR-324-null mice
Authors: DJ Hayman, T Modebadze, S Charlton, K Cheung, J Soul, H Lin, Y Hao, CG Miles, D Tsompani, RM Jackson, MD Briggs, KA Piróg, IM Clark, MJ Barter, GJ Clowry, FEN LeBeau, DA Young
Scientific Reports, 2021-05-17;11(1):10452.
Species: Mouse
Sample Types: Tissue Lysates
Applications: Western Blot
Mouse LIMR3/CD300f is a negative regulator of the antimicrobial activity of neutrophils
Authors: K Ueno, M Urai, K Izawa, Y Otani, N Yanagihara, M Kataoka, S Takatsuka, M Abe, H Hasegawa, K Shimizu, T Kitamura, J Kitaura, Y Miyazaki, Y Kinjo
Sci Rep, 2018-11-27;8(1):17406.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot
An activating and inhibitory signal from an inhibitory receptor LMIR3/CLM-1: LMIR3 augments lipopolysaccharide response through association with FcRgamma in mast cells.
Authors: Izawa K, Kitaura J, Yamanishi Y, Matsuoka T, Kaitani A, Sugiuchi M, Takahashi M, Maehara A, Enomoto Y, Oki T, Takai T, Kitamura T
J. Immunol., 2009-06-26;183(2):925-36.
Species: Mouse
Sample Types: Cell Lysates, Whole Cells
Applications: Functional Assay, Immunoprecipitation, Western Blot
The major targets of acute norovirus infection are immune cells in the gut-associated lymphoid tissue
Authors: KR Grau, AN Roth, S Zhu, A Hernandez, N Colliou, BB DiVita, DT Philip, C Riffe, B Giasson, SM Wallet, M Mohamadzad, SM Karst
Nat Microbiol, 2017-11-06;0(0):.
Environmentally-triggered contraction of the norovirus virion determines diarrheagenic potential
Authors: Emily W. Helm, Amy M. Peiper, Matthew Phillips, Caroline G. Williams, Michael B. Sherman, Theresa Kelley et al.
Frontiers in Immunology
Functional receptor molecules CD300lf and CD300ld within the CD300 family enable murine noroviruses to infect cells
Authors: Kei Haga, Akira Fujimoto, Reiko Takai-Todaka, Motohiro Miki, Yen Hai Doan, Kosuke Murakami et al.
Proceedings of the National Academy of Sciences