Home / CD63 / Mouse CD63 Antibody

Mouse CD63 Antibody

Catalog #: MAB5417
6 Citations 2 Reviews Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB5417
MAB5417-SP
Detection of CD63 in HEK293 Human Cell Line Transfected with Mouse CD63 and eGFP by Flow Cytometry.
2 Images
Product Details
Citations (6)
FAQs
Supplemental Products
Reviews (2)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse CD63 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse CD63 in direct ELISAs.
Source
Monoclonal Rat IgG1 Clone # 446703
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
HEK293 human embryonic kidney cell line transfected with mouse CD63
Met1-Met238
Accession # P41731
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/mL
See below
Immunohistochemistry
8-25 µg/mL
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of CD63 antibody in HEK293 Human Cell Line Transfected with Mouse CD63 and eGFP antibody by Flow Cytometry. View Larger

Detection of CD63 in HEK293 Human Cell Line Transfected with Mouse CD63 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with either (A) mouse CD63 or (B) irrelevant transfectants and eGFP were stained with Rat Anti-Mouse CD63 Monoclonal Antibody (Catalog # MAB5417) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). Quadrant markers were set based on control antibody staining (Catalog # MAB005). View our protocol for Staining Membrane-associated Proteins.

Immunohistochemistry CD63 antibody in Mouse Kidney by Immunohistochemistry (IHC-Fr). View Larger

CD63 in Mouse Kidney. CD63 was detected in perfusion fixed frozen sections of mouse kidney using Rat Anti-Mouse CD63 Monoclonal Antibody (Catalog # MAB5417) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Specific labeling was localized to the plasma membrane of epithelial cells in convoluted tubules. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Loading...
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD63

CD63, also known as LAMP-3 or ME491 (melanoma-associated antigen), is a 30-60 kDa member of the tetraspanin superfamily of protein trafficking proteins. CD63 is ubiquitously expressed and found in late endocytic vesicles, but following cell activation is also present on the plasma membrane. Interaction of CD63 with other membrane proteins or adaptors regulates cell activities such as adhesion, migration and degranulation. Extracellular regions of mouse CD63 share 94% and 67% amino acid sequence identity with rat and human CD63, respectively.

Entrez Gene IDs
967 (Human); 12512 (Mouse); 29186 (Rat); 474391 (Canine)
Alternate Names
CD_antigen: CD63; CD63 antigen (melanoma 1 antigen); CD63 antigen; CD63 molecule; CD63; Granulophysin; Lamp-3; Lysosomal-associated membrane protein 3; lysosome-associated membrane glycoprotein 3; ME491; melanoma 1 antigen; Melanoma-associated antigen ME491; melanoma-associated antigen MLA1; MLA1; Ocular melanoma-associated antigen; OMA81H; tetraspanin-30; Tspan30; tspan-30

Product Datasheets

You must select a language.

x
Product Datasheet
COA
SDS

Citations for Mouse CD63 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
Filter your results:

Filter by:

  1. Human alveolar type 2 epithelium transdifferentiates into metaplastic KRT5+ basal cells
    Authors: JJ Kathiriya, C Wang, M Zhou, A Brumwell, M Cassandras, CJ Le Saux, M Cohen, KD Alysandrat, B Wang, P Wolters, M Matthay, DN Kotton, HA Chapman, T Peng
    Nature Cell Biology, 2021-12-30;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  2. Exosome reporter mice reveal the involvement of exosomes in mediating neuron to astroglia communication in the CNS
    Authors: Y Men, J Yelick, S Jin, Y Tian, MSR Chiang, H Higashimor, E Brown, R Jarvis, Y Yang
    Nat Commun, 2019-09-12;10(1):4136.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  3. Bacterial colonization of host cells in the absence of cholesterol.
    Authors: Gilk S, Cockrell D, Luterbach C, Hansen B, Knodler L, Ibarra J, Steele-Mortimer O, Heinzen R
    PLoS Pathog, 2013-01-24;9(1):e1003107.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: IHC
  4. TIMP-1 promotes hypermigration of Toxoplasma-infected primary dendritic cells via CD63 / ITGB1 / FAK signaling
    Authors: EB Ólafsson, EC Ross, M Varas-Godo, A Barragan
    J. Cell. Sci., 2019-02-04;0(0):.
  5. Nanotube‐like processes facilitate material transfer between photoreceptors
    Authors: Aikaterini A Kalargyrou, Mark Basche, Aura Hare, Emma L West, Alexander J Smith, Robin R Ali et al.
    EMBO reports
  6. Extracellular vesicles released from irradiated neonatal mouse cheek tissue increased cell survival after radiation
    Authors: Kentaro Ariyoshi, Yota Hiroyama, Naoya Fujiwara, Tomisato Miura, Kosuke Kasai, Akifumi Nakata et al.
    Journal of Radiation Research

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs
Loading...

Reviews for Mouse CD63 Antibody

Average Rating: 5 (Based on 2 Reviews)

5 Star
100%
4 Star
0%
3 Star
0%
2 Star
0%
1 Star
0%

Have you used Mouse CD63 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Filter by:


Mouse CD63 Antibody
By Anonymous on 10/26/2021
Application: IHC Sample Tested: glioblastoma Species: Mouse
Immunohistochemistry Mouse CD63 Antibody MAB5417
Mouse CD63 Antibody
By Anonymous on 09/28/2019
Application: WB Sample Tested: Recombinant protein Species: Mouse
Western Blot Mouse CD63 Antibody MAB5417