Mouse CD96 Antibody

Name: Mouse CD96 Antibody
Brand: R&D Systems
SKU: AF5690
Price: 449 USD

Catalog #: AF5690 Datasheet / COA / SDS

Discontinued Product

AF5690 has been discontinued.

View all CD96 products.

Detection of Mouse CD96 by Western Blot.

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All CD96 Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse CD96 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse CD96 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human CD96 is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse CD96
Glu25-Met536
Accession # AAH52865

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Mouse CD96 by Western Blot. Western blot shows lysates of CTLL-2 mouse cytotoxic T cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse CD96 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5690) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for CD96 at approximately 170 - 180 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD96

Mouse CD96 (also Tactile) is a 170‑180 kDa member of the Ig-Superfamily. It is expressed on CD4⁺ and CD8⁺ T cells, NK and NKT cells, resting monocytes and gamma δ T cells. Mouse CD96 binds to CD155 and nectin-1, and likely participates in cell-to-cell adhesion. Mature mouse CD96 is a 581 amino acid (aa), type I transmembrane glycoprotein. It contains a 515 aa extracellular region (aa 22‑536) that contains three Ig-like domains, plus a 45 aa cytoplasmic region. The two N-terminal domains are V‑type (aa 24‑244), while the distal domain is a C-type structure (aa 250‑355). Unlike human, there is no splice variant in the second V‑type domain. There is, however, a potential isoform that shows a single Cys substitution for aa 437‑602. Over aa 1‑536, mouse CD96 shares 55% and 79% aa identity with human and rat CD96, respectively.

Entrez Gene IDs

10225 (Human); 84544 (Mouse); 498079 (Rat); 102121080 (Cynomolgus Monkey)

Alternate Names

CD96 antigenMGC22596; CD96 molecule; CD96; DKFZp667E2122; T cell-activated increased late expression protein; TACTILE; TACTILEincreased late expression; T-cell surface protein tactile