Mouse CXCL17/VCC-1 Antibody

Catalog #: MAB4270 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB4270
MAB4270-SP

Detection of Mouse CXCL17 by Western Blot.

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All CXCL17/VCC-1 Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse CXCL17/VCC-1 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects recombinant mouse (rm) CXCL17/VCC‑1 in direct ELISAs and Western blots. In direct ELISAs, 50% cross‑reactivity with recombinant human CXCL17 is observed/VCC-1.

Source

Monoclonal Rat IgG_2B Clone # 510614

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant mouse CXCL17/VCC‑1
Ser31-Leu119
Accession # Q5UW37

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Mouse CXCL17 by Western Blot. Western blot shows lysates of mouse lung tissue. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse CXCL17 Monoclonal Antibody (Catalog # MAB4270) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for CXCL17 at approximately 11 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1 (with 3% BSA instead of 5% milk in the Antibody Solution).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CXCL17/VCC-1

CXCL17, also known as dendritic cell and monocyte chemokine-like protein (DMC) and VEGF-correlated chemokine-1 (VCC-1), is a secreted molecule with a size and predicted three-dimensional folding pattern similar to that of chemokines CXCL8/IL-8 and CXCL14/BRAK (1, 2). It has no predicted N-glycosylation sites, so cleavage of a 22 amino acid (aa) signal sequence likely results in a mature mouse CXCL17 molecule of 97 aa and 11 kDa size. CXCL17 is constitutively produced by airway and intestinal epithelium (1). It induces the chemotaxis of quiescent, but not LPS‑activated peripheral blood monocytes and dendritic cells, and also binds these cells specifically (1). CXCL17 expression is increased in endothelial cells when they are induced to form tubes in vitro (2). Transgenic overexpression in NIH3T3 cells causes upregulation of proteins such as VEGF and FGF basic, and increases cell growth rate and tumorigenicity (2). CXCL17 and two other chemokines that play roles in angiogenesis, CXCL1/GRO and CXCL8/IL-8, show significantly correlated expression with that of VEGF in primary lung, breast and esophageal tumors (2). CXCL17 is therefore suggested to play a role in tumor angiogenesis. Mature mouse CXCL17 shares 82%, 71% and 66% amino acid sequence identity with rat, human and bovine CXCL17, respectively.

References

Pisabarro, M. T. et al. (2006) J. Immunol. 176:2069.
Weinstein, E. J. et al. (2006) Biochem. Biophys. Res. Commun. 350:74.

Entrez Gene IDs

284340 (Human); 232983 (Mouse)

Alternate Names

Chemokine (C-X-C Motif) Ligand 17; C-X-C Motif Chemokine 17; CXCL17; Dcip1; Dendritic Cell And Monocyte Chemokine-Like Protein; DMC; UNQ473; VCC1; VCC-1; VEGF Coregulated Chemokine 1; VEGF Co-Regulated Chemokine 1