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Mouse CXCL2/GRO beta /MIP-2/CINC-3 Biotinylated Antibody

Catalog #: BAF452
19 Citations 1 Review Datasheet / COA / SDS
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BAF452
CXCL2/GRO beta /MIP‑2/CINC‑3 in Mouse Splenocytes.
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Citations (19)
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Reviews (1)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse CXCL2/GRO beta /MIP-2/CINC-3 Biotinylated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse CXCL2/MIP-2 in ELISAs and Western blots. In sandwich immunoassays, less than 0.05% cross-reactivity with recombinant human (rh) GRO beta, rhGRO gamma, recombinant mouse (rm) C10, rmMIP-1 alpha, rmMIP-1 beta, rhRANTES, and rmKC is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse CXCL2/MIP-2 (R&D Systems, Catalog # 452-M2)
Ala28-Asn100
Accession # P10889
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse CXCL2/GRO beta /MIP‑2/CINC‑3 (Catalog # 452-M2)
Immunohistochemistry
5-15 µg/mL
See below
Immunocytochemistry
5-15 µg/mL
See below

Mouse CXCL2/MIP-2 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Detection (Matched Antibody Pair)
0.1-0.4 µg/mL 

Use in combination with:

Capture Reagent: Mouse CXCL2/GRO beta /MIP‑2/CINC‑3 Antibody (Catalog # MAB452)

Standard: Recombinant Mouse CXCL2/MIP-2 Protein (Catalog # 452-M2)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry CXCL2/GRO beta /MIP-2/CINC-3 antibody in Mouse Splenocytes by Immunocytochemistry (ICC). View Larger

CXCL2/GRO beta /MIP‑2/CINC‑3 in Mouse Splenocytes. CXCL2/GRO beta /MIP-2/CINC-3 was detected in immersion fixed mouse splenocytes stimulated with LPS and monensin using Mouse CXCL2/GRO beta /MIP-2/CINC-3 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF452) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Immunohistochemistry CXCL2/GRO beta /MIP-2/CINC-3 antibody in Mouse Spleen by Immunohistochemistry (IHC-Fr). View Larger

CXCL2/GRO beta /MIP‑2/CINC‑3 in Mouse Spleen. CXCL2/GRO beta /MIP-2/CINC-3 was detected in immersion fixed frozen sections of mouse spleen using Mouse CXCL2/GRO beta /MIP-2/CINC-3 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF452) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CXCL2/GRO beta/MIP-2/CINC-3

Macrophage Inflammatory Protein-2 (MIP-2) was originally identified as a heparin-binding protein secreted from a murine macrophage cell line in response to endotoxin stimulation. Based on its protein and DNA sequences, MIP-2 is a member of the alpha (C-X-C) subfamily of chemokines.

MIP-2 cDNA encodes a 100 amino acid residue precursor protein from which the amino-terminal 27 amino acid residues are cleaved to generate the mature MIP-2. The protein sequence of murine MIP-2 shows approximately 63% identity to that of murine KC, another murine alpha chemokine whose expression is induced by PDGF. In addition, the protein sequence of MIP-2 is also 60% identical to human GRO beta and GRO gamma. It has been suggested that mouse KC and MIP-2 are the homologs of the human GROs and rat CINCs.

Similarly to other alpha chemokines, murine MIP-2 is a potent neutrophil attractant and activator. MIP-2 and KC can bind the murine interleukin 8 type B receptor homologue with high affinity. The expression of MIP-2 was found to be associated with neutrophil influx in pulmonary inflammation and glomerulonephritis, suggesting that MIP-2 may contribute to the pathogenesis of inflammatory diseases.

Entrez Gene IDs
2920 (Human); 20310 (Mouse); 114105 (Rat)
Alternate Names
chemokine (C-X-C motif) ligand 2; CINC-2a; CINC3; CINC-3; C-X-C Motif Chemokine 2; CXCL2; GRO beta; GRO2 oncogene; GRO2; GROB; Gro-beta; Growth-regulated protein beta; Macrophage inflammatory protein 2-alpha; melanoma growth stimulatory activity beta; MGSA beta; MGSA-b; MGSA-beta; MIP2; MIP-2; MIP2A; MIP-2a; MIP2-alpha; SCYB2

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Citations for Mouse CXCL2/GRO beta /MIP-2/CINC-3 Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

19 Citations: Showing 1 - 10
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  1. Early growth response 1 deficiency protects host against Pseudomonas aeruginosa lung infection
    Authors: Z Pang, R Raudonis, C McCormick, Z Cheng
    Infect. Immun., 2019-12-17;0(0):.
    Species: Mouse
    Sample Types: BALF
    Applications: ELISA Detection
  2. Modulation of double-stranded RNA pattern recognition receptor signaling in ovarian cancer cells promotes inflammatory queues
    Authors: M Muccioli, H Nandigam, T Loftus, M Singh, A Venkatesh, J Wright, M Pate, K McCall, F Benencia
    Oncotarget, 2018-11-30;9(94):36666-36683.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development (Detection)
  3. Regulation of inflammatory factors by double-stranded RNA receptors in breast cancer cells
    Authors: A Venkatesh, H Nandigam, M Muccioli, M Singh, T Loftus, D Lewis, M Pate, F Benencia
    Immunobiology, 2017-11-22;0(0):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development (Detection)
  4. The calcineurin-NFAT axis contributes to host defense during Pseudomonas aeruginosa lung infection
    Authors: Z Pang, RD Junkins, AJ MacNeil, C McCormick, Z Cheng, WM Chen, TJ Lin
    J. Leukoc. Biol., 2017-10-10;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: ELISA Development (Detection)
  5. IL-33 Attenuates Sepsis by Inhibiting IL-17 Receptor Signaling through Upregulation of SOCS3
    Authors: R Lv, J Zhao, M Lei, D Xiao, Y Yu, J Xie
    Cell. Physiol. Biochem., 2017-08-09;42(5):1961-1972.
    Species: Mouse
    Sample Types: Serum
    Applications: ELISA Development (Detection)
  6. Pulmonary Epithelial Cell-Derived Cytokine TGF-beta1 Is a Critical Cofactor for Enhanced Innate Lymphoid Cell Function.
    Authors: Denney L, Byrne A, Shea T, Buckley J, Pease J, Herledan G, Walker S, Gregory L, Lloyd C
    Immunity, 2015-11-17;43(5):945-58.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: ELISA Development (Detection)
  7. Regulator of calcineurin 1 suppresses inflammation during respiratory tract infections.
    Authors: Junkins R, MacNeil A, Wu Z, McCormick C, Lin T
    J Immunol, 2013-04-15;190(10):5178-86.
    Species: Mouse
    Sample Types: BALF
    Applications: ELISA Development (Detection)
  8. Diesel exhaust particulates exacerbate asthma-like inflammation by increasing CXC chemokines.
    Authors: Kim J, Natarajan S, Vaickus LJ, Bouchard JC, Beal D, Cruikshank WW, Remick DG
    Am. J. Pathol., 2011-10-01;179(6):2730-9.
    Species: Mouse
    Sample Types: BALF
    Applications: ELISA Development
  9. Increased cytokine production in IL-18 receptor alpha-deficient cells is associated with dysregulation of suppressors of cytokine signaling (SOCS).
    Authors: Nold-Petry CA, Nold MF, Nielsen JW, Bustamante A, Zepp JA, Storm KA, Hong JW, Kim SH, Dinarello CA
    J. Biol. Chem., 2009-07-10;0(0):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  10. ST2 deficient mice display a normal host defense against pulmonary infection with Mycobacterium tuberculosis.
    Authors: Wieland CW, van der Windt GJ, Florquin S, McKenzie AN, van der Poll T
    Microbes Infect., 2009-03-13;11(4):524-30.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  11. CD14 contributes to pulmonary inflammation and mortality during murine tuberculosis.
    Authors: Wieland CW, van der Windt GJ, Wiersinga WJ, Florquin S, van der Poll T
    Immunology, 2008-04-03;125(2):272-9.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: ELISA Development
  12. Overexpression of suppressor of cytokine signaling-5 in T cells augments innate immunity during septic peritonitis.
    Authors: Watanabe H, Kubo M, Numata K, Takagi K, Mizuta H, Okada S, Ito T, Matsukawa A
    J. Immunol., 2006-12-15;177(12):8650-7.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: ELISA Development
  13. Lack of in vitro and in vivo recognition of Francisella tularensis subspecies lipopolysaccharide by Toll-like receptors.
    Authors: Hajjar AM, Harvey MD, Shaffer SA, Goodlett DR, Sjostedt A, Edebro H, Forsman M, Bystrom M, Pelletier M, Wilson CB, Miller SI, Skerrett SJ, Ernst RK
    Infect. Immun., 2006-09-18;74(12):6730-8.
    Species: Mouse
    Sample Types: BALF
    Applications: ELISA Development
  14. Neutrophil recruitment in immunized mice depends on MIP-2 inducing the sequential release of MIP-1alpha, TNF-alpha and LTB(4).
    Authors: Ramos CD, Fernandes KS, Canetti C, Teixeira MM, Silva JS, Cunha FQ
    Eur. J. Immunol., 2006-08-01;36(8):2025-34.
    Species: Mouse
    Sample Types: Tissue Secretion
    Applications: ELISA Development
  15. Circulating cytokine/inhibitor profiles reshape the understanding of the SIRS/CARS continuum in sepsis and predict mortality.
    Authors: Osuchowski MF, Welch K, Siddiqui J, Remick DG
    J. Immunol., 2006-08-01;177(3):1967-74.
    Species: Mouse
    Sample Types: Plasma
    Applications: ELISA Development
  16. Adenoviral-mediated overexpression of SOCS3 enhances IgG immune complex-induced acute lung injury.
    Authors: Gao H, Hoesel LM, Guo RF, Rancilio NJ, Sarma JV, Ward PA
    J. Immunol., 2006-07-01;177(1):612-20.
    Species: Mouse
    Sample Types: BALF
    Applications: ELISA Development
  17. Matrix metalloproteinase-8 deficiency promotes granulocytic allergen-induced airway inflammation.
    Authors: Gueders MM, Balbin M, Rocks N, Foidart JM, Gosset P, Louis R, Shapiro S, Lopez-Otin C, Noel A, Cataldo DD
    J. Immunol., 2005-08-15;175(4):2589-97.
    Species: Mouse
    Sample Types: BALF
    Applications: ELISA Development
  18. Sequential ELISA to profile multiple cytokines from small volumes.
    Authors: Osuchowski MF, Siddiqui J, Copeland S, Remick DG
    J. Immunol. Methods, 2005-07-01;302(1):172-81.
    Species: Mouse
    Sample Types: Plasma
    Applications: ELISA Development
  19. Transcriptional profiling of lipopolysaccharide-induced acute lung injury.
    Authors: Jeyaseelan S, Chu HW, Young SK, Worthen GS
    Infect. Immun., 2004-12-01;72(12):7247-56.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: ELISA Development

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Mouse CXCL2/GRO beta /MIP-2/CINC-3 Biotinylated Antibody
By Anonymous on 08/07/2019
Application: ELISA Sample Tested: Serum Species: Mouse
ELISA Mouse CXCL2/GRO beta /MIP-2/CINC-3 Biotinylated Antibody BAF452