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Mouse CXCL9/MIG Antibody

Catalog #: AF-492-NA
31 Citations Datasheet / COA / SDS
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AF-492-NA
AF-492-SP
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Chemotaxis Induced by CXCL9/MIG and Neutralization by Mouse CXCL9/MIG Antibody.
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Mouse CXCL9/MIG Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse CXCL9/MIG in ELISAs. In sandwich immunoassays, less than 0.5% cross-reactivity with recombinant human (rh) CXCL9/MIG is observed and less than 0.005% cross-reactivity with recombinant mouse MIP-2, recombinant rat CINC-1, rhGRO alpha, rhGRO beta, and rhGRO gamma is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse CXCL9/MIG
Accession # P18340
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample

Mouse CXCL9/MIG Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
0.2-0.8 µg/mL 

Use in combination with:

Detection Reagent: Mouse CXCL9/MIG Biotinylated Antibody (Catalog # BAF492)

Standard: Recombinant Mouse CXCL9/MIG Protein (Catalog # 492-MM)

Neutralization
Measured by its ability to neutralize CXCL9/MIG-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with mouse CXCR3. The Neutralization Dose (ND50) is typically 6-20 µg/mL in the presence of 1 µg/mL Recombinant Mouse CXCL9/MIG.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Chemotaxis Induced by CXCL9/MIG and Neutralization by Mouse CXCL9/MIG Antibody. View Larger

Chemotaxis Induced by CXCL9/MIG and Neutralization by Mouse CXCL9/MIG Antibody. Recombinant Mouse CXCL9/MIG (Catalog # 492-MM) chemoattracts the BaF3 mouse pro-B cell line transfected with mouse CXCR3 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CXCL9/MIG (1 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse CXCL9/MIG Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-492-NA). The ND50 is typically 6-20 µg/mL.

Immunocytochemistry/ Immunofluorescence Detection of Mouse CXCL9/MIG by Immunocytochemistry/ Immunofluorescence View Larger

Detection of Mouse CXCL9/MIG by Immunocytochemistry/ Immunofluorescence mTORC1 regulates Cxcl9 in osteoblasts.(a) Representative images of in situ hybridization of Cxcl9 mRNA in conjunction with immunostaining of Runx2 in femur sections of 12-week-old male mice bone. Boxed area is enlarged in the bottom right corner. Cxcl9+ osteoblasts out of total osteoblasts were also quantified. Scale bar, 50 μm. n=9 per group. (b) Representative photomicrographs of immunostaining of CXCR3 in CD31+ ECs in bone marrow and quantitative analysis of CXCR3+ ECs out of total ECs in 12-week-old male mice bone. Scale bar, 50 μm. n=9 per group. (c) Representative photomicrographs of immunostaining of CXCR3 in cultured HUVECs. Scale bar, 100 μm. (d) Cxcl9 concentrations assessed by ELISA in bone marrow (BM) and serum. n=5 per group. (e) Quantitative PCR analysis of Cxcl9 mRNA in primary osteoblasts. (f) Western blot of Cxcl9 in primary osteoblasts. (g) Concentrations of Cxcl9 in CM of primary osteoblasts assessed by ELISA. n=5 per group. Data are shown as mean±s.d. *P<0.05, **P<0.01 (Student's t-test). Ctrl, control. Image collected and cropped by CiteAb from the following open publication (https://www.nature.com/articles/ncomms13885), licensed under a CC-BY license. Not internally tested by R&D Systems.

Western Blot Detection of Mouse CXCL9/MIG by Western Blot View Larger

Detection of Mouse CXCL9/MIG by Western Blot mTORC1 regulates Cxcl9 in osteoblasts.(a) Representative images of in situ hybridization of Cxcl9 mRNA in conjunction with immunostaining of Runx2 in femur sections of 12-week-old male mice bone. Boxed area is enlarged in the bottom right corner. Cxcl9+ osteoblasts out of total osteoblasts were also quantified. Scale bar, 50 μm. n=9 per group. (b) Representative photomicrographs of immunostaining of CXCR3 in CD31+ ECs in bone marrow and quantitative analysis of CXCR3+ ECs out of total ECs in 12-week-old male mice bone. Scale bar, 50 μm. n=9 per group. (c) Representative photomicrographs of immunostaining of CXCR3 in cultured HUVECs. Scale bar, 100 μm. (d) Cxcl9 concentrations assessed by ELISA in bone marrow (BM) and serum. n=5 per group. (e) Quantitative PCR analysis of Cxcl9 mRNA in primary osteoblasts. (f) Western blot of Cxcl9 in primary osteoblasts. (g) Concentrations of Cxcl9 in CM of primary osteoblasts assessed by ELISA. n=5 per group. Data are shown as mean±s.d. *P<0.05, **P<0.01 (Student's t-test). Ctrl, control. Image collected and cropped by CiteAb from the following open publication (https://www.nature.com/articles/ncomms13885), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunocytochemistry/ Immunofluorescence Detection of Mouse CXCL9/MIG by Immunocytochemistry/ Immunofluorescence View Larger

Detection of Mouse CXCL9/MIG by Immunocytochemistry/ Immunofluorescence mTORC1 regulates Cxcl9 in osteoblasts.(a) Representative images of in situ hybridization of Cxcl9 mRNA in conjunction with immunostaining of Runx2 in femur sections of 12-week-old male mice bone. Boxed area is enlarged in the bottom right corner. Cxcl9+ osteoblasts out of total osteoblasts were also quantified. Scale bar, 50 μm. n=9 per group. (b) Representative photomicrographs of immunostaining of CXCR3 in CD31+ ECs in bone marrow and quantitative analysis of CXCR3+ ECs out of total ECs in 12-week-old male mice bone. Scale bar, 50 μm. n=9 per group. (c) Representative photomicrographs of immunostaining of CXCR3 in cultured HUVECs. Scale bar, 100 μm. (d) Cxcl9 concentrations assessed by ELISA in bone marrow (BM) and serum. n=5 per group. (e) Quantitative PCR analysis of Cxcl9 mRNA in primary osteoblasts. (f) Western blot of Cxcl9 in primary osteoblasts. (g) Concentrations of Cxcl9 in CM of primary osteoblasts assessed by ELISA. n=5 per group. Data are shown as mean±s.d. *P<0.05, **P<0.01 (Student's t-test). Ctrl, control. Image collected and cropped by CiteAb from the following open publication (https://www.nature.com/articles/ncomms13885), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CXCL9/MIG

CXCL9, also known as MIG, is a member of the alpha  subfamily of chemokines that lacks the ELR domain, and was initially identified as a lymphokine-activated gene in mouse macrophages. Human CXCL9 was subsequently cloned using mouse CXCL9 cDNA as a probe. The CXCL9 gene is induced in macrophages and in primary glial cells of the central nervous system specifically in response to IFN-gamma. CXCL9 has been shown to be a chemoattractant for activated T-lymphocytes and TIL but not for neutrophils or monocytes. The mouse CXCL9 cDNA encodes a 126 amino acid residue precursor protein with a 21 amino acid residue signal peptide that is cleaved to yield a 105 amino acid residue mature protein. CXCL9 has an extended carboxy-terminus containing greater than 50% basic amino acid residues and is larger than most other chemokines. The carboxy-terminal residues of CXCL9 are prone to proteolytic cleavage resulting in size heterogeneity of natural and recombinant CXCL9. CXCL9 with large carboxy-terminal deletions have been shown to have diminished activity in the calcium flux assay. A chemokine receptor (CXCR3) specific for CXCL9 and IP-10 has been cloned and shown to be highly expressed in IL-2-activated T-lymphocytes.

Entrez Gene IDs
4283 (Human); 17329 (Mouse); 246759 (Rat)
Alternate Names
chemokine (C-X-C motif) ligand 9; CMK; crg-10; C-X-C motif chemokine 9; CXCL9; Gamma-interferon-induced monokine; Humig; MIG; MIGSmall-inducible cytokine B9; monokine induced by gamma interferon; SCYB9Monokine induced by interferon-gamma

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Citations for Mouse CXCL9/MIG Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

31 Citations: Showing 1 - 10
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  1. FGFR3 alterations in bladder cancer stimulate serine synthesis to induce immune-inert macrophages that suppress T-cell recruitment and activation
    Authors: Ouyang, Y;Ou, Z;Zhong, W;Yang, J;Fu, S;Ouyang, N;Chen, J;Xu, L;Wu, D;Qian, J;Lin, Y;Lin, T;Huang, J;
    Cancer research
    Species: Human, Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  2. Human adipose-derived mesenchymal stem cells prevent type 1 diabetes induced by immune checkpoint blockade
    Authors: Emi Kawada-Horitani, Shunbun Kita, Tomonori Okita, Yuto Nakamura, Hiroyuki Nishida, Yoichi Honma et al.
    Diabetologia
  3. MYC inhibition reprograms tumor immune microenvironment by recruiting T lymphocytes and activating the CD40/CD40L system in osteosarcoma
    Authors: Kuo Jiang, Qianfeng Zhang, Yong Fan, Jia Li, Jitao Zhang, Wentao Wang et al.
    Cell Death Discovery
  4. Activating a collaborative innate-adaptive immune response to control metastasis
    Authors: Lijuan Sun, Tim Kees, Ana Santos Almeida, Bodu Liu, Xue-Yan He, David Ng et al.
    Cancer Cell
  5. Targeting KDM4A epigenetically activates tumor-cell-intrinsic immunity by inducing DNA replication stress
    Authors: Wuchang Zhang, Wei Liu, Lingfei Jia, Demeng Chen, Insoon Chang, Michael Lake et al.
    Molecular Cell
  6. Inhibition of the CXCL9-CXCR3 axis suppresses the progression of experimental apical periodontitis by blocking macrophage migration and activation
    Authors: T Hasegawa, V Venkata Su, Y Yahata, M Nakano, S Suzuki, S Suzuki, S Yamada, H Kitaura, I Mizoguchi, Y Noiri, K Handa, M Saito
    Scientific Reports, 2021-01-28;11(1):2613.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  7. Commensal-driven immune zonation of the liver promotes host defence
    Authors: A Gola, MG Dorrington, E Speranza, C Sala, RM Shih, AJ Radtke, HS Wong, AP Baptista, JM Hernandez, G Castellani, IDC Fraser, RN Germain
    Nature, 2020-11-25;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC

  8. Increased Osteoblastic Cxcl9 Contributes to the Uncoupled Bone Formation and Resorption in Postmenopausal Osteoporosis
    Authors: Zezheng Liu, Wenquan Liang, Dawei Kang, Qingjing Chen, Zhicong Ouyang, Huibo Yan et al.
    Clinical Interventions in Aging
  9. LIF regulates CXCL9 in tumor-associated macrophages and prevents CD8+ T cell tumor-infiltration impairing anti-PD1 therapy
    Authors: M Pascual-Ga, E Bonfill-Te, E Planas-Rig, C Rubio-Pere, R Iurlaro, A Arias, I Cuartas, A Sala-Hojma, L Escudero, F Martínez-R, I Huber-Ruan, P Nuciforo, L Pedrosa, C Marques, I Braña, E Garralda, M Vieito, M Squatrito, E Pineda, F Graus, C Espejo, J Sahuquillo, J Tabernero, J Seoane
    Nat Commun, 2019-06-11;10(1):2416.
    Species: Mouse
    Sample Types: In Vivo, Whole Cells
    Applications: ICC, Neutralization
  10. Herpes Simplex Virus Type 2 Infection-Induced Expression of CXCR3 Ligands Promotes CD4+ T Cell Migration and Is Regulated by the Viral Immediate-Early Protein ICP4
    Authors: Mudan Zhang, Xu Deng, Xinmeng Guan, Lanlan Geng, Ming Fu, Binman Zhang et al.
    Frontiers in Immunology
  11. Herpes simplex virus-1 evasion of CD8+ T cell accumulation contributes to viral encephalitis
    Authors: N Koyanagi, T Imai, K Shindo, A Sato, W Fujii, T Ichinohe, N Takemura, S Kakuta, S Uematsu, H Kiyono, Y Maruzuru, J Arii, A Kato, Y Kawaguchi
    J. Clin. Invest., 2017-09-11;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  12. IL-33 Attenuates Sepsis by Inhibiting IL-17 Receptor Signaling through Upregulation of SOCS3
    Authors: R Lv, J Zhao, M Lei, D Xiao, Y Yu, J Xie
    Cell. Physiol. Biochem., 2017-08-09;42(5):1961-1972.
    Species: Mouse
    Sample Types: Serum
    Applications: ELISA Development (Capture)
  13. Optimal CD4 T cell priming after LPS-based adjuvanticity with CD134 costimulation relies on CXCL9 production
    Authors: P Shinde, W Liu, A Ménoret, AD Luster, AT Vella
    J. Leukoc. Biol., 2017-04-21;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  14. Salt suppresses IFN? inducible chemokines through the IFN?-JAK1-STAT1 signaling pathway in proximal tubular cells
    Authors: Y Arai, D Takahashi, K Asano, M Tanaka, M Oda, SBH Ko, MSH Ko, S Mandai, N Nomura, T Rai, S Uchida, E Sohara
    Sci Rep, 2017-04-20;7(0):46580.
    Species: Mouse
    Sample Types: Tissue Homogenates, Whole Tissue
    Applications: IHC, Western Blot
  15. Osteoblasts secrete Cxcl9 to regulate angiogenesis in bone
    Nat Commun, 2016-12-14;7(0):13885.
    Species: Mouse
    Sample Types: Cell Lysates, In Vivo, Whole Cells
    Applications: Neutralization, Western Blot
  16. A Novel PD-L1-targeting Antagonistic DNA Aptamer With Antitumor Effects
    Authors: Wei-Yun Lai
    Mol Ther Nucleic Acids, 2016-12-13;5(12):e397.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  17. CXCR3 Blockade Inhibits T Cell Migration into the Skin and Prevents Development of Alopecia Areata
    J Immunol, 2016-07-13;0(0):.
    Species: Mouse
    Sample Types: Whole Cells, Whole Tissue
    Applications: Flow Cytometry, IHC-P
  18. Regulatory T cells prevent inducible BALT formation by dampening neutrophilic inflammation.
    Authors: Foo S, Zhang V, Lalwani A, Lynch J, Zhuang A, Lam C, Foster P, King C, Steptoe R, Mazzone S, Sly P, Phipps S
    J Immunol, 2015-03-25;194(9):4567-76.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  19. Combination of C-X-C motif chemokine 9 and C-X-C motif chemokine 10 antibodies with FTY720 prolongs the survival of cardiac retransplantation allografts in a mouse model
    Authors: TENG MA, JIACHENG XU, JIAWEI ZHUANG, XIAOBIAO ZHOU, LIANFENG LIN, ZHONGGUI SHAN et al.
    Experimental and Therapeutic Medicine
  20. Desiccating Stress–Induced Chemokine Expression in the Epithelium Is Dependent on Upregulation of NKG2D/RAE-1 and Release of IFN-gamma in Experimental Dry Eye
    Authors: Terry G. Coursey, Ritu Bohat, Flavia L. Barbosa, Stephen C. Pflugfelder, Cintia S. de Paiva
    The Journal of Immunology
  21. Tumor cell-activated CARD9 signaling contributes to metastasis-associated macrophage polarization.
    Authors: Yang M, Shao J, Miao Y, Cui W, Qi Y, Han J, Lin X, Du J
    Cell Death Differ, 2014-04-11;21(8):1290-302.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  22. Astrocyte-Derived CXCL10 Drives Accumulation of Antibody-Secreting Cells in the Central Nervous System during Viral Encephalomyelitis
    Authors: Timothy W. Phares, Stephen A. Stohlman, David R. Hinton, Cornelia C. Bergmann
    Journal of Virology
  23. Trafficking of high avidity HER-2/neu-specific T cells into HER-2/neu-expressing tumors after depletion of effector/memory-like regulatory T cells.
    Authors: Weiss VL, Lee TH, Song H, Kouo TS, Black CM, Sgouros G, Jaffee EM, Armstrong TD
    PLoS ONE, 2012-02-16;7(2):e31962.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  24. A novel combination immunotherapy for cancer by IL-13Ralpha2-targeted DNA vaccine and immunotoxin in murine tumor models.
    Authors: Nakashima H, Terabe M, Berzofsky JA, Husain SR, Puri RK
    J. Immunol., 2011-10-17;187(10):4935-46.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  25. Comprehensive assessment of chemokine expression profiles by flow cytometry.
    Authors: Eberlein J, Nguyen TT, Victorino F, Golden-Mason L, Rosen HR, Homann D
    J. Clin. Invest., 2010-02-08;120(3):907-23.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  26. Monokine induced by interferon-gamma (MIG/CXCL9) is derived from both donor and recipient sources during rejection of class II major histocompatibility complex disparate skin allografts.
    Authors: Auerbach MB, Shimoda N, Amano H, Rosenblum JM, Kish DD, Farber JM, Fairchild RL
    Am. J. Pathol., 2009-04-23;174(6):2172-81.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  27. L-selectin-negative CCR7- effector and memory CD8+ T cells enter reactive lymph nodes and kill dendritic cells.
    Authors: Guarda G, Hons M, Soriano SF, Huang AY, Polley R, Martin-Fontecha A, Stein JV, Germain RN, Lanzavecchia A, Sallusto F
    Nat. Immunol., 2007-05-27;8(7):743-52.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  28. The direct action of 1alpha,25(OH)2-vitamin D3 on purified mouse Langerhans cells.
    Authors: Fujita H, Asahina A, Komine M, Tamaki K
    Cell. Immunol., 2007-05-15;245(2):70-9.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  29. Viral infection of the lungs through the eye.
    Authors: Bitko V, Musiyenko A, Barik S
    J. Virol., 2006-10-18;81(2):783-90.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  30. IL-21 induces tumor rejection by specific CTL and IFN-gamma-dependent CXC chemokines in syngeneic mice.
    Authors: Di Carlo E, Comes A, Orengo AM, Rosso O, Meazza R, Musiani P, Colombo MP, Ferrini S
    J. Immunol., 2004-02-01;172(3):1540-7.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  31. Tumor necrosis factor-dependent segmental control of MIG expression by high endothelial venules in inflamed lymph nodes regulates monocyte recruitment.
    Authors: Janatpour MJ, Hudak S, Sathe M, McEvoy LM
    J. Exp. Med., 2001-11-05;194(9):1375-84.
    Species: Mouse
    Sample Types: In Vivo, Whole Tissue
    Applications: IHC, Neutralization

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