Mouse CXCR4 Antibody

Catalog #: MAB21651R Datasheet / COA / SDS

Recombinant Monoclonal Antibody

Catalog #	Availability	Size / Price	Qty
MAB21651R-100
MAB21651R-SP

Detection of CXCR4 in HEK293 Human Cell Line Transfected with Mouse CXCR4 and eGFP by Flow Cytometry.

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All CXCR4 Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse CXCR4 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse CXCR4 transfectants. Does not stain irrelevant transfectants.

Source

Recombinant Monoclonal Rat IgG_2B Clone # 247506R

Purification

Protein A or G purified from cell culture supernatant

Immunogen

Y3 rat myeloid cell line transfected with mouse CXCR4
Met1-Ser359
Accession # P70658

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

See below

Immunohistochemistry

5-25 µg/mL

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Immunocytochemistry

1-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of CXCR4 in HEK293 Human Cell Line Transfected with Mouse CXCR4 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with mouse CXCR4 and eGFP was stained with (A) Rat Anti-Mouse CXCR4 Monoclonal Antibody (Catalog # MAB21651R) or (B) Rat IgG2B isotype control antibody (Catalog # MAB0061) followed by APC-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry

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Detection of CXCR4 in Mouse Thymocytes by Flow Cytometry. Mouse thymocytes were stained with Rat Anti-Mouse CXCR4 Monoclonal Antibody (Catalog # MAB21651R, filled histogram) or Rat IgG2B isotype control antibody (Catalog # MAB0061, open histogram) followed by Goat anti-Rat IgG PE-conjugated Secondary Antibody (Catalog # F0105B). View our protocol for Staining Membrane-associated Proteins.

Immunocytochemistry

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CXCR4 in Mouse Splenocytes. CXCR4 was detected in immersion fixed mouse splenocytes using Rat Anti-Mouse CXCR4 Monoclonal Antibody (Catalog # MAB21651R) at 1 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Immunohistochemistry

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CXCR4 in Mouse Spleen. CXCR4 was detected in perfusion fixed frozen sections of mouse spleen using Rat Anti-Mouse CXCR4 Monoclonal Antibody (Catalog # MAB21651R) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to splenocytes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Immunohistochemistry

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CXCR4 in Mouse Testis. CXCR4 was detected in perfusion fixed frozen sections of mouse testis using Rat Anti-Mouse CXCR4 Monoclonal Antibody (Catalog # MAB21651R) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to cell membranes in sperm cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CXCR4

CXCR4, also known as CD184, is a G-protein-linked seven transmembrane spanning receptor that binds stromal cell-derived factor-1 (SDF-1). CXCR4 acts as a co-factor for T-cell tropic HIV-1 and -2 viral entry into cells. While primarily a membrane protein, CXCR4 undergoes trafficking and internalization in response to stimulation with phorbol esters and ligand (1). Cytoplasmic and nuclear localization of CXCR4 has been observed in colorectal and renal carcinomas (2,3) and it has been used as the basis of prognosis and metastatic state (3,4,5).

References

Orsini, M.J. et al. (1999) J. Biol. Chem. 274:31076.
Zagzag, D. et al. (2005) Cancer Res. 65:6178.
Speetjens, F.M. et al. (2009) Cancer Microenvironment 2:1.
Wang, L. et al. (2009) Oncology Reports 22:1333.
Amara, S. et al. (2015) Cancer Biomark. 15:869.

Long Name

C-X-C Motif Chemokine Receptor 4

Entrez Gene IDs

7852 (Human); 12767 (Mouse); 60628 (Rat); 483900 (Canine); 493676 (Feline)

Alternate Names

CD184; chemokine (C-X-C motif) receptor 4; chemokine (C-X-C motif), receptor 4 (fusin); C-X-C chemokine receptor type 4; CXCR4; CXC-R4; CXCR-4; D2S201E; D2S201ESDF-1 receptor; FB22; Fusin; HM89; HM89NPYRL; HSY3RR; LAP3; LAP-3; LCR1; LESTR; LESTRCD184 antigen; Leukocyte-derived seven transmembrane domain receptor; leukocyte-derived seven-transmembrane-domain receptor; lipopolysaccharide-associated protein 3; neuropeptide Y receptor Y3; NPY3R; NPY3RWHIM; NPYR; NPYRL; NPYY3R; pCXCR4; seven transmembrane helix receptor; seven-transmembrane-segment receptor, spleen; Stromal cell-derived factor 1 receptor; WHIMS