Mouse Dectin-2/CLEC6A alpha Isoform Biotinylated Antibody Summary
Phe43-Leu209
Accession # Q9JKF4
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Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Dectin-2/CLEC6A
Dectin-2 alpha, also known as CLEC6A, CLECSF10, and NKCL, belongs to the C-type lectin family of transmembrane immune regulatory glycoproteins. Dectin-2 alpha, CLEC4A/DCIR, CLEC4B/DCAR, CLEC4C/DLEC, CLEC4D/MCL, and CLEC4E/mincle constitute a subgroup of these molecules that exhibit approximately 40% amino acid (aa) sequence identity in their extracellular domains (ECD) and have a conserved cysteine spacing in their carbohydrate recognition domains (CRD) (1, 2). Mature mouse Dectin-2 alpha consists of a short cytoplasmic tail, a transmembrane segment, and an ECD with a stalk region and one CRD (3, 4). Alternate splicing leads to partial deletion of the transmembrane segment and stalk ( beta isoform) or a portion of the CRD ( gamma isoform) (4). The full length Dectin-2 alpha isoform is a 27 kDa molecule that is primarily expressed on the surface of tissue macrophages and their precursors (3‑6). The CRD of Dectin-2 alpha contains an EPN motif which is characteristic of calcium-dependent mannose-binding lectins. Dectin-2 alpha selectively interacts with high mannose structures in the Man9GlcNAc2 configuration (7). It mediates the recognition of a variety of microorganisms, particularly the filamentous forms of yeast and fungii (7, 8). The short cytoplasmic tail does not contain signaling motifs but mediates association with the ITAM-containing Fc receptor gamma subunit in macrophages (8). Ligation of Dectin-2 alpha induces tyrosine phosphorylation of the gamma subunit, activation of NF kappa B, and enhanced release of TNF-alpha and IL-1ra (8). Macrophage Dectin-2 alpha is upregulated in vivo by inflammatory stimuli and UV-B irradiation (6). It mediates the breaking of UV-induced tolerance by interacting with CD4+CD25+ regulatory T cells which then induce dendritic cells to release IL-4, IL-10, and TGF-beta (9). Within the ECD, mouse Dectin-2 alpha shares 71% aa sequence identity with human and bovine Dectin-2.
- Kanazawa, N. (2007) J. Dermatol. Sci. 45:77.
- Kanazawa, N. et al. (2004) Immunobiology 209:179.
- Fernandes, M.J. et al. (1999) Cancer Res. 59:2709.
- Ariizumi, K. et al. (2000) J. Biol. Chem. 275:11957.
- Bonkobara, M. et al. (2005) Photochem. Photobiol. 81:944.
- Taylor, P.R. et al. (2005) Eur. J. Immunol. 35:2163.
- McGreal, E.P. et al. (2006) Glycobiology 16:422.
- Sato, K. et al. (2006) J. Biol. Chem. 281:38854.
- Aragane, Y. et al. (2003) J. Immunol. 171:3801.
Product Datasheets
Citations for Mouse Dectin-2/CLEC6A alpha Isoform Biotinylated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 3
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Neutrophils require SKAP2 for reactive oxygen species production following C-type lectin and Candida stimulation
Authors: Giang T. Nguyen, Shuying Xu, Walter Adams, John M. Leong, Stephen C. Bunnell, Michael K. Mansour et al.
iScience
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The balance between gasdermin D and STING signaling shapes the severity of schistosome immunopathology
Authors: P Kalantari, I Shecter, J Hopkins, A Pilotta Go, Y Morales, BF Harandi, S Sharma, MJ Stadecker
Proceedings of the National Academy of Sciences of the United States of America, 2023-03-21;120(13):e2211047120.
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CD209a Synergizes with Dectin-2 and Mincle to Drive Severe Th17 Cell-Mediated Schistosome Egg-Induced Immunopathology
Authors: P Kalantari, Y Morales, EA Miller, LD Jaramillo, HE Ponichtera, MA Wuethrich, C Cheong, MC Seminario, JM Russo, SC Bunnell, MJ Stadecker
Cell Rep, 2018-01-30;22(5):1288-1300.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot
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