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Mouse E-Cadherin Biotinylated Antibody

Catalog #: BAF748
4 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
BAF748
E‑Cadherin in A431 Human Cell Line.
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Citations (4)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse E-Cadherin Biotinylated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse E-Cadherin in ELISAs and Western blots. In sandwich immunoassays, less than 1% cross-reactivity with recombinant human (rh) E-Cadherin, rhN-Cadherin, recombinant mouse (rm) P-Cadherin and rmVE-Cadherin is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse E-Cadherin
Asp157-Val709
Accession # P09803
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse E-Cadherin Fc Chimera (Catalog # 748-EC)
Immunohistochemistry
5-15 µg/mL
Perfusion fixed frozen sections of mouse spinal cord
Immunocytochemistry
5-15 µg/mL
See below

Mouse E-Cadherin Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Detection (Matched Antibody Pair)
0.1-0.4 µg/mL 

Use in combination with:

Capture Reagent: Mouse E‑Cadherin Antibody (Catalog # MAB7482)

Standard: Recombinant Mouse E-Cadherin Fc Chimera Protein, CF (Catalog # 748-EC)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry E-Cadherin antibody in A431 Human Cell Line by Immunocytochemistry (ICC). View Larger

E‑Cadherin in A431 Human Cell Line. E-Cadherin was detected in immersion fixed A431 human skin epidermal cell line using Goat Anti-Mouse E-Cadherin Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF748) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (yellow; Catalog # NL999) and counterstained with DAPI (blue). Specific staining was localized to plasma membranes. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: E-Cadherin

Epithelial (E) - Cadherin (ECAD), also known as cell-CAM120/80 in the human, uvomorulin in the mouse, Arc-1 in the dog, and L-CAM in the chicken, is a member of the cadherin family of cell adhesion molecules. Cadherins are calcium-dependent transmembrane proteins, which bind to one another in a homophilic manner. On their cytoplasmic side, they associate with the three catenins, alpha, beta, and gamma (plakoglobin). This association links the cadherin protein to the cytoskeleton. Without association with the catenins, the cadherins are non-adhesive. Cadherins play a role in development, specifically in tissue formation. They may also help to maintain tissue architecture in the adult. E-Cadherin may also play a role in tumor development, as loss of E-Cadherin has been associated with tumor invasiveness. E-Cadherin is a classical cadherin molecule. Classical cadherins consist of a large extracellular domain which contains DXD and DXNDN repeats responsible for mediating calcium‑dependent adhesion, a single-pass transmembrane domain, and a short carboxy-terminal cytoplasmic domain responsible for interacting with the catenins. E‑Cadherin contains five extracellular calcium-binding domains of approximately 110 amino acids each.

References
  1. Bussemakers, M.J.G. et al. (1993) Mol. Biol. Reports 17:123.
  2. Overduin, M. et al. (1995) Science 267:386.
  3. Takeichi, M. (1991) 251:1451.
Entrez Gene IDs
999 (Human); 12550 (Mouse); 83502 (Rat)
Alternate Names
Arc-1; CAD1; cadherin 1, E-cadherin (epithelial); cadherin 1, type 1, E-cadherin (epithelial); Cadherin-1; calcium-dependent adhesion protein, epithelial; CAM 120/80; CD324 antigen; CD324; CDH1; CDHE; cell-CAM 120/80; Cell-CAM120/80; ECAD; ECadherin; E-Cadherin; Epithelial cadherin; LCAM; L-CAM; UVOE-Cadherin; Uvomorulin

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Citations for Mouse E-Cadherin Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Reduced frequencies and heightened CD103 expression among virus-induced CD8(+) T cells in the respiratory tract airways of vitamin A-deficient mice
    Authors: Rajeev Rudraraju, Sherri L. Surman, Bart G. Jones, Robert Sealy, David L. Woodland, Julia L. Hurwitz
    Clinical and Vaccine Immunology
  2. Combined mutation of Apc, Kras and Tgfbr2 effectively drives metastasis of intestinal cancer
    Authors: E Sakai, M Nakayama, H Oshima, Y Kouyama, A Niida, S Fujii, A Ochiai, KI Nakayama, K Mimori, Y Suzuki, CP Hong, CY Ock, SJ Kim, M Oshima
    Cancer Res., 2017-12-27;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  3. Quantifying cell-generated mechanical forces within living embryonic tissues.
    Authors: Campas O, Mammoto T, Hasso S, Sperling R, O'Connell D, Bischof A, Maas R, Weitz D, Mahadevan L, Ingber D
    Nat Methods, 2013-12-08;11(2):183-9.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  4. Mesp1 coordinately regulates cardiovascular fate restriction and epithelial-mesenchymal transition in differentiating ESCs.
    Authors: Lindsley RC, Gill JG, Murphy TL, Langer EM, Cai M, Mashayekhi M, Wang W, Niwa N, Nerbonne JM, Kyba M, Murphy KM
    Cell Stem Cell, 2008-07-03;3(1):55-68.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC

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